Does the mouse tail vein injection method provide a good model of lung cancer? [version 1; peer review: 2 approved]
Lung cancer drug development requires screening in animal models. We aimed to develop orthotopic models of human non-small lung cancer using A549 and H3122 cells delivered by tail vein injection. This procedure has been used previously for a mouse lung cancer (Lewis lung carcinoma) and as a model of...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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F1000 Research Ltd
2019-02-01
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| Series: | F1000Research |
| Online Access: | https://f1000research.com/articles/8-190/v1 |
| _version_ | 1818202708732018688 |
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| author | Nensi Shrestha Zabeen Lateef Orleans Martey Abigail R. Bland Mhairi Nimick Rhonda Rosengren John C. Ashton |
| author_facet | Nensi Shrestha Zabeen Lateef Orleans Martey Abigail R. Bland Mhairi Nimick Rhonda Rosengren John C. Ashton |
| author_sort | Nensi Shrestha |
| collection | DOAJ |
| description | Lung cancer drug development requires screening in animal models. We aimed to develop orthotopic models of human non-small lung cancer using A549 and H3122 cells delivered by tail vein injection. This procedure has been used previously for a mouse lung cancer (Lewis lung carcinoma) and as a model of human breast cancer metastasis to lung. We report that the procedure led to poor animal condition 7-8 weeks after injection, and produced lesions in the lungs visible at necropsy but we were unable identify individual cancer cells using immunohistochemistry. We conclude that if this method is to produce a model that can be used in drug experiments, improvements are required for cancer cell detection post mortem, such as by using of a fluorescently tagged human lung cancer cell line. |
| first_indexed | 2024-12-12T03:13:45Z |
| format | Article |
| id | doaj.art-ea9d4aefef0546e585750238b98f57ec |
| institution | Directory Open Access Journal |
| issn | 2046-1402 |
| language | English |
| last_indexed | 2024-12-12T03:13:45Z |
| publishDate | 2019-02-01 |
| publisher | F1000 Research Ltd |
| record_format | Article |
| series | F1000Research |
| spelling | doaj.art-ea9d4aefef0546e585750238b98f57ec2022-12-22T00:40:21ZengF1000 Research LtdF1000Research2046-14022019-02-01810.12688/f1000research.17964.119647Does the mouse tail vein injection method provide a good model of lung cancer? [version 1; peer review: 2 approved]Nensi Shrestha0Zabeen Lateef1Orleans Martey2Abigail R. Bland3Mhairi Nimick4Rhonda Rosengren5John C. Ashton6Department of Pharmacology and Toxicology, University of Otago, Dunedin, 9054, New ZealandDepartment of Pharmacology and Toxicology, University of Otago, Dunedin, 9054, New ZealandDepartment of Pharmacology and Toxicology, University of Otago, Dunedin, 9054, New ZealandDepartment of Pharmacology and Toxicology, University of Otago, Dunedin, 9054, New ZealandDepartment of Pharmacology and Toxicology, University of Otago, Dunedin, 9054, New ZealandDepartment of Pharmacology and Toxicology, University of Otago, Dunedin, 9054, New ZealandDepartment of Pharmacology and Toxicology, University of Otago, Dunedin, 9054, New ZealandLung cancer drug development requires screening in animal models. We aimed to develop orthotopic models of human non-small lung cancer using A549 and H3122 cells delivered by tail vein injection. This procedure has been used previously for a mouse lung cancer (Lewis lung carcinoma) and as a model of human breast cancer metastasis to lung. We report that the procedure led to poor animal condition 7-8 weeks after injection, and produced lesions in the lungs visible at necropsy but we were unable identify individual cancer cells using immunohistochemistry. We conclude that if this method is to produce a model that can be used in drug experiments, improvements are required for cancer cell detection post mortem, such as by using of a fluorescently tagged human lung cancer cell line.https://f1000research.com/articles/8-190/v1 |
| spellingShingle | Nensi Shrestha Zabeen Lateef Orleans Martey Abigail R. Bland Mhairi Nimick Rhonda Rosengren John C. Ashton Does the mouse tail vein injection method provide a good model of lung cancer? [version 1; peer review: 2 approved] F1000Research |
| title | Does the mouse tail vein injection method provide a good model of lung cancer? [version 1; peer review: 2 approved] |
| title_full | Does the mouse tail vein injection method provide a good model of lung cancer? [version 1; peer review: 2 approved] |
| title_fullStr | Does the mouse tail vein injection method provide a good model of lung cancer? [version 1; peer review: 2 approved] |
| title_full_unstemmed | Does the mouse tail vein injection method provide a good model of lung cancer? [version 1; peer review: 2 approved] |
| title_short | Does the mouse tail vein injection method provide a good model of lung cancer? [version 1; peer review: 2 approved] |
| title_sort | does the mouse tail vein injection method provide a good model of lung cancer version 1 peer review 2 approved |
| url | https://f1000research.com/articles/8-190/v1 |
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