Baseline Susceptibility, Cross-Resistance, and Sublethal Effects of Broflanilide, a Novel Meta-Diamide Pesticide, in <i>Spodoptera litura</i>
<i>Spodoptera litura</i> is a damaging and notorious insect pest of agricultural crops that has developed resistance to various insecticides. Broflanilide is a novel pesticide with a unique mode of action that displays high efficiency against lepidopterous larvae. We here determined the...
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MDPI AG
2023-03-01
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| Series: | International Journal of Molecular Sciences |
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| author | Yunyi Li Cheng Qu Qinghe Zhang Liping Zhang Chen Luo Ran Wang |
| author_facet | Yunyi Li Cheng Qu Qinghe Zhang Liping Zhang Chen Luo Ran Wang |
| author_sort | Yunyi Li |
| collection | DOAJ |
| description | <i>Spodoptera litura</i> is a damaging and notorious insect pest of agricultural crops that has developed resistance to various insecticides. Broflanilide is a novel pesticide with a unique mode of action that displays high efficiency against lepidopterous larvae. We here determined the baseline susceptibility of a laboratory strain of <i>S</i>. <i>litura</i> to broflanilide and 10 other popular insecticides. Furthermore, we measured susceptibility and cross-resistance using three common insecticides in 11 field-collected <i>S</i>. <i>litura</i> populations. Broflanilide caused the highest toxicity among all tested insecticides, with the laboratory strain and all field-collected populations showing high susceptibility. Moreover, no cross-resistance was detected between broflanilide and the other tested insecticides. We subsequently evaluated the sublethal effects of broflanilide and found that treatment with the 25% lethal concentration (LC<sub>25</sub>) prolonged the development duration in the larvae, reduced the pupation rate and pupae weight, and decreased egg hatchability. Finally, the activities of three detoxifying enzymes were measured in <i>S</i>. <i>litura</i> after treatment with the LC<sub>25</sub> dose. The results suggested that enhanced cytochrome P450 monooxygenase (P450) activity could be involved in broflanilide detoxification. Overall, these findings demonstrate the strong toxicity and significant sublethal effects of broflanilide in <i>S</i>. <i>litura</i> and suggest that increased P450 activity may be associated with broflanilide detoxification. |
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| issn | 1661-6596 1422-0067 |
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| last_indexed | 2024-03-11T06:26:35Z |
| publishDate | 2023-03-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-ea9f49fd4c18454684cd94a1c80377a92023-11-17T11:32:42ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-03-01246535110.3390/ijms24065351Baseline Susceptibility, Cross-Resistance, and Sublethal Effects of Broflanilide, a Novel Meta-Diamide Pesticide, in <i>Spodoptera litura</i>Yunyi Li0Cheng Qu1Qinghe Zhang2Liping Zhang3Chen Luo4Ran Wang5Institute of Cotton Research, Shanxi Agricultural University, Yuncheng 044000, ChinaInstitute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaInstitute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaInstitute of Cotton Research, Shanxi Agricultural University, Yuncheng 044000, ChinaInstitute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaInstitute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China<i>Spodoptera litura</i> is a damaging and notorious insect pest of agricultural crops that has developed resistance to various insecticides. Broflanilide is a novel pesticide with a unique mode of action that displays high efficiency against lepidopterous larvae. We here determined the baseline susceptibility of a laboratory strain of <i>S</i>. <i>litura</i> to broflanilide and 10 other popular insecticides. Furthermore, we measured susceptibility and cross-resistance using three common insecticides in 11 field-collected <i>S</i>. <i>litura</i> populations. Broflanilide caused the highest toxicity among all tested insecticides, with the laboratory strain and all field-collected populations showing high susceptibility. Moreover, no cross-resistance was detected between broflanilide and the other tested insecticides. We subsequently evaluated the sublethal effects of broflanilide and found that treatment with the 25% lethal concentration (LC<sub>25</sub>) prolonged the development duration in the larvae, reduced the pupation rate and pupae weight, and decreased egg hatchability. Finally, the activities of three detoxifying enzymes were measured in <i>S</i>. <i>litura</i> after treatment with the LC<sub>25</sub> dose. The results suggested that enhanced cytochrome P450 monooxygenase (P450) activity could be involved in broflanilide detoxification. Overall, these findings demonstrate the strong toxicity and significant sublethal effects of broflanilide in <i>S</i>. <i>litura</i> and suggest that increased P450 activity may be associated with broflanilide detoxification.https://www.mdpi.com/1422-0067/24/6/5351<i>Spodoptera litura</i>insecticide sensitivityfield-evolved resistancecross-resistancesublethal effectsdetoxifying enzymes |
| spellingShingle | Yunyi Li Cheng Qu Qinghe Zhang Liping Zhang Chen Luo Ran Wang Baseline Susceptibility, Cross-Resistance, and Sublethal Effects of Broflanilide, a Novel Meta-Diamide Pesticide, in <i>Spodoptera litura</i> International Journal of Molecular Sciences <i>Spodoptera litura</i> insecticide sensitivity field-evolved resistance cross-resistance sublethal effects detoxifying enzymes |
| title | Baseline Susceptibility, Cross-Resistance, and Sublethal Effects of Broflanilide, a Novel Meta-Diamide Pesticide, in <i>Spodoptera litura</i> |
| title_full | Baseline Susceptibility, Cross-Resistance, and Sublethal Effects of Broflanilide, a Novel Meta-Diamide Pesticide, in <i>Spodoptera litura</i> |
| title_fullStr | Baseline Susceptibility, Cross-Resistance, and Sublethal Effects of Broflanilide, a Novel Meta-Diamide Pesticide, in <i>Spodoptera litura</i> |
| title_full_unstemmed | Baseline Susceptibility, Cross-Resistance, and Sublethal Effects of Broflanilide, a Novel Meta-Diamide Pesticide, in <i>Spodoptera litura</i> |
| title_short | Baseline Susceptibility, Cross-Resistance, and Sublethal Effects of Broflanilide, a Novel Meta-Diamide Pesticide, in <i>Spodoptera litura</i> |
| title_sort | baseline susceptibility cross resistance and sublethal effects of broflanilide a novel meta diamide pesticide in i spodoptera litura i |
| topic | <i>Spodoptera litura</i> insecticide sensitivity field-evolved resistance cross-resistance sublethal effects detoxifying enzymes |
| url | https://www.mdpi.com/1422-0067/24/6/5351 |
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