Seed priming and exogenous application of citric acid enhance seedling growth and photosynthetic pigments and mitigate oxidative damage of soybean (Glycine max) under salt stress
Seed priming and citric acid (CA) supplementation on germination and seedling growth of soybeans were investigated. Soybean seeds were primed with distilled water (control), 1 mM CA (CA1), or 2 mM CA (CA2) and then placed for germination in Petri dishes containing distilled water or 150 mM NaCl (SS)...
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| Format: | Article |
| Language: | English |
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University of Belgrade, University of Novi Sad
2023-01-01
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| Series: | Archives of Biological Sciences |
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| Online Access: | https://doiserbia.nb.rs/img/doi/0354-4664/2023/0354-46642300033I.pdf |
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| author | Imran Shahin Mahamud Asif Chandra Paul Newton Chakrobortty Jotirmoy Sarker Prosenjit Paul Shipan Tahjib-Ul-Arif Saidur Rhaman Mohammad |
| author_facet | Imran Shahin Mahamud Asif Chandra Paul Newton Chakrobortty Jotirmoy Sarker Prosenjit Paul Shipan Tahjib-Ul-Arif Saidur Rhaman Mohammad |
| author_sort | Imran Shahin |
| collection | DOAJ |
| description | Seed priming and citric acid (CA) supplementation on germination and seedling growth of soybeans were investigated. Soybean seeds were primed with distilled water (control), 1 mM CA (CA1), or 2 mM CA (CA2) and then placed for germination in Petri dishes containing distilled water or 150 mM NaCl (SS), alone or in combination with 1 mM or 2 mM CA. Germinated seeds were placed in hydroponic pots using a similar treatment regimen to that specified for the Petri dishes to obtain seedling growth and biochemical parameters. Salt stress significantly lowered germination, growth traits, relative water content (RWC), and photosynthetic pigment. When soybean seeds were primed with CA under salt stress, the germination rate, final germination percentage, seed vigor index, and number of lateral roots significantly increased. Moreover, supplementation of CA significantly increased fresh and dry shoot and root weight, plant height, RWC, and photosynthetic pigments compared to salt-treated plants. The results also displayed that salt stress considerably increased hydrogen peroxide (H2O2) and malondialdehyde (MDA) contents compared to control plants. Spraying of CA1 and CA2 significantly lowered the levels of H2O2 and MDA in salt-treated plants. Both hierarchical clustering and PCA revealed that the effects of salt stress and CA on germination, growth characteristics, photosynthetic pigments, H2O2, and MDA concentrations strongly interacted with one another. According to the findings, CA could be applied as a seed priming and exogenous agent to help soybeans grow more quickly when exposed to salt stress. |
| first_indexed | 2024-04-24T11:31:31Z |
| format | Article |
| id | doaj.art-eaa35b5526724894a816e5afbde01925 |
| institution | Directory Open Access Journal |
| issn | 0354-4664 1821-4339 |
| language | English |
| last_indexed | 2024-04-24T11:31:31Z |
| publishDate | 2023-01-01 |
| publisher | University of Belgrade, University of Novi Sad |
| record_format | Article |
| series | Archives of Biological Sciences |
| spelling | doaj.art-eaa35b5526724894a816e5afbde019252024-04-10T10:15:17ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392023-01-0175440741810.2298/ABS230804033I0354-46642300033ISeed priming and exogenous application of citric acid enhance seedling growth and photosynthetic pigments and mitigate oxidative damage of soybean (Glycine max) under salt stressImran Shahin0Mahamud Asif1Chandra Paul Newton2Chakrobortty Jotirmoy3Sarker Prosenjit4Paul Shipan5Tahjib-Ul-Arif6Saidur Rhaman Mohammad7Department of Agronomy, Khulna Agricultural University, Khulna, Bangladesh + Institute of Plant Science and Resources, Okayama University, Chuo, Kurashiki, JapanDepartment of Agricultural Chemistry, Khulna Agricultural University, Khulna, BangladeshDepartment of Agronomy, Khulna Agricultural University, Khulna, BangladeshDepartment of Soil Science, Khulna Agricultural University, Khulna, BangladeshDepartment of Crop Botany, Khulna Agricultural University, Khulna, BangladeshDepartment of Biochemistry and Molecular Biology, Bangladesh Agricultural University, Mymensingh, BangladeshDepartment of Biochemistry and Molecular Biology, Bangladesh Agricultural University, Mymensingh, BangladeshDepartment of Seed Science and Technology, Bangladesh Agricultural University, Mymensingh, Bangladesh + Peking University Institute of Advanced Agricultural Science, Shandong Laboratory of Advanced Agricultural Science at Weifang, Weifang, Shandong, ChinaSeed priming and citric acid (CA) supplementation on germination and seedling growth of soybeans were investigated. Soybean seeds were primed with distilled water (control), 1 mM CA (CA1), or 2 mM CA (CA2) and then placed for germination in Petri dishes containing distilled water or 150 mM NaCl (SS), alone or in combination with 1 mM or 2 mM CA. Germinated seeds were placed in hydroponic pots using a similar treatment regimen to that specified for the Petri dishes to obtain seedling growth and biochemical parameters. Salt stress significantly lowered germination, growth traits, relative water content (RWC), and photosynthetic pigment. When soybean seeds were primed with CA under salt stress, the germination rate, final germination percentage, seed vigor index, and number of lateral roots significantly increased. Moreover, supplementation of CA significantly increased fresh and dry shoot and root weight, plant height, RWC, and photosynthetic pigments compared to salt-treated plants. The results also displayed that salt stress considerably increased hydrogen peroxide (H2O2) and malondialdehyde (MDA) contents compared to control plants. Spraying of CA1 and CA2 significantly lowered the levels of H2O2 and MDA in salt-treated plants. Both hierarchical clustering and PCA revealed that the effects of salt stress and CA on germination, growth characteristics, photosynthetic pigments, H2O2, and MDA concentrations strongly interacted with one another. According to the findings, CA could be applied as a seed priming and exogenous agent to help soybeans grow more quickly when exposed to salt stress.https://doiserbia.nb.rs/img/doi/0354-4664/2023/0354-46642300033I.pdfcitric acidgerminationphotosynthetic pigmentsseed vigor indexsalt stress |
| spellingShingle | Imran Shahin Mahamud Asif Chandra Paul Newton Chakrobortty Jotirmoy Sarker Prosenjit Paul Shipan Tahjib-Ul-Arif Saidur Rhaman Mohammad Seed priming and exogenous application of citric acid enhance seedling growth and photosynthetic pigments and mitigate oxidative damage of soybean (Glycine max) under salt stress Archives of Biological Sciences citric acid germination photosynthetic pigments seed vigor index salt stress |
| title | Seed priming and exogenous application of citric acid enhance seedling growth and photosynthetic pigments and mitigate oxidative damage of soybean (Glycine max) under salt stress |
| title_full | Seed priming and exogenous application of citric acid enhance seedling growth and photosynthetic pigments and mitigate oxidative damage of soybean (Glycine max) under salt stress |
| title_fullStr | Seed priming and exogenous application of citric acid enhance seedling growth and photosynthetic pigments and mitigate oxidative damage of soybean (Glycine max) under salt stress |
| title_full_unstemmed | Seed priming and exogenous application of citric acid enhance seedling growth and photosynthetic pigments and mitigate oxidative damage of soybean (Glycine max) under salt stress |
| title_short | Seed priming and exogenous application of citric acid enhance seedling growth and photosynthetic pigments and mitigate oxidative damage of soybean (Glycine max) under salt stress |
| title_sort | seed priming and exogenous application of citric acid enhance seedling growth and photosynthetic pigments and mitigate oxidative damage of soybean glycine max under salt stress |
| topic | citric acid germination photosynthetic pigments seed vigor index salt stress |
| url | https://doiserbia.nb.rs/img/doi/0354-4664/2023/0354-46642300033I.pdf |
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