Cerebrospinal Fluid Levels of AFP and hCG: Validation of the Analytical Method and Application in the Diagnosis of Central Nervous System Germ Cell Tumors
The determination of Human Chorionic Gonadotropin (hCG) and Alpha Fetoprotein (AFP) levels on serum and amniotic fluid plays a fundamental role in the diagnosis and follow-up of specific physiological or pathological conditions (e.g., pregnancy, threat of abortion or germ cell tumors). Recently, the...
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2021-10-01
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| Series: | Diagnostics |
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| author | Annamaria D'Alessandro Domenico Ciavardelli Anna Pastore Germana Giannone Giada Del Baldo Andrea Carai Angela Mastronuzzi Andrea Onetti Muda Ottavia Porzio |
| author_facet | Annamaria D'Alessandro Domenico Ciavardelli Anna Pastore Germana Giannone Giada Del Baldo Andrea Carai Angela Mastronuzzi Andrea Onetti Muda Ottavia Porzio |
| author_sort | Annamaria D'Alessandro |
| collection | DOAJ |
| description | The determination of Human Chorionic Gonadotropin (hCG) and Alpha Fetoprotein (AFP) levels on serum and amniotic fluid plays a fundamental role in the diagnosis and follow-up of specific physiological or pathological conditions (e.g., pregnancy, threat of abortion or germ cell tumors). Recently, the quantification of hCG and AFP in other biological fluids has gained great attention to support the diagnosis, prognosis and follow-up of neoplastic diseases deriving from trophoblastic cells, such as germinomas. Most of the commercial kits for hCG and AFP assays are developed to be used on biological fluids such as serum/plasma and/or urine by manufacturing companies. The aim of this work was to evaluate the suitability of the analytical method certified for the use on serum, and/or amniotic fluid for the quantification of hCG and AFP in cerebrospinal fluid, carrying out an internal validation protocol. The data reported here show that the automated immunochemical method is fit for quantification of hCG and AFP in cerebrospinal fluid (CSF), allowing selective and specific diagnosis of secreting germ cell tumors. This is confirmed by the positive correlation between elevated levels of hCG or AFP and the diagnosis of brain tumors. |
| first_indexed | 2024-03-10T05:34:55Z |
| format | Article |
| id | doaj.art-eaa7a5fb9003413fb1b4ac9128126353 |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-10T05:34:55Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-eaa7a5fb9003413fb1b4ac91281263532023-11-22T23:00:36ZengMDPI AGDiagnostics2075-44182021-10-011111198010.3390/diagnostics11111980Cerebrospinal Fluid Levels of AFP and hCG: Validation of the Analytical Method and Application in the Diagnosis of Central Nervous System Germ Cell TumorsAnnamaria D'Alessandro0Domenico Ciavardelli1Anna Pastore2Germana Giannone3Giada Del Baldo4Andrea Carai5Angela Mastronuzzi6Andrea Onetti Muda7Ottavia Porzio8Clinical Biochemistry Laboratory, IRCCS “Bambino Gesù” Children’s Hospital, 00165 Rome, ItalySchool of Medicine and Surgery, University “Kore” of Enna, 94100 Enna, ItalyResearch Unit of Diagnostical and Management Innovations, IRCCS “Bambino Gesù” Children’s Hospital, 00165 Rome, ItalyClinical Biochemistry Laboratory, IRCCS “Bambino Gesù” Children’s Hospital, 00165 Rome, ItalyDepartment of Onco-Hematology, Cell Therapy, Gene Therapy and Hemopoietic Transplant, IRCCS “Bambino Gesù” Children’s Hospital, 00165 Rome, ItalyDepartment of Neurosciences, IRCCS “Bambino Gesù” Children’s Hospital, 00165 Rome, ItalyDepartment of Onco-Hematology, Cell Therapy, Gene Therapy and Hemopoietic Transplant, IRCCS “Bambino Gesù” Children’s Hospital, 00165 Rome, ItalyResearch Unit of Diagnostical and Management Innovations, IRCCS “Bambino Gesù” Children’s Hospital, 00165 Rome, ItalyClinical Biochemistry Laboratory, IRCCS “Bambino Gesù” Children’s Hospital, 00165 Rome, ItalyThe determination of Human Chorionic Gonadotropin (hCG) and Alpha Fetoprotein (AFP) levels on serum and amniotic fluid plays a fundamental role in the diagnosis and follow-up of specific physiological or pathological conditions (e.g., pregnancy, threat of abortion or germ cell tumors). Recently, the quantification of hCG and AFP in other biological fluids has gained great attention to support the diagnosis, prognosis and follow-up of neoplastic diseases deriving from trophoblastic cells, such as germinomas. Most of the commercial kits for hCG and AFP assays are developed to be used on biological fluids such as serum/plasma and/or urine by manufacturing companies. The aim of this work was to evaluate the suitability of the analytical method certified for the use on serum, and/or amniotic fluid for the quantification of hCG and AFP in cerebrospinal fluid, carrying out an internal validation protocol. The data reported here show that the automated immunochemical method is fit for quantification of hCG and AFP in cerebrospinal fluid (CSF), allowing selective and specific diagnosis of secreting germ cell tumors. This is confirmed by the positive correlation between elevated levels of hCG or AFP and the diagnosis of brain tumors.https://www.mdpi.com/2075-4418/11/11/1980AFPhCGcerebrospinal fluidgerm cell tumorscentral nervous system tumors |
| spellingShingle | Annamaria D'Alessandro Domenico Ciavardelli Anna Pastore Germana Giannone Giada Del Baldo Andrea Carai Angela Mastronuzzi Andrea Onetti Muda Ottavia Porzio Cerebrospinal Fluid Levels of AFP and hCG: Validation of the Analytical Method and Application in the Diagnosis of Central Nervous System Germ Cell Tumors Diagnostics AFP hCG cerebrospinal fluid germ cell tumors central nervous system tumors |
| title | Cerebrospinal Fluid Levels of AFP and hCG: Validation of the Analytical Method and Application in the Diagnosis of Central Nervous System Germ Cell Tumors |
| title_full | Cerebrospinal Fluid Levels of AFP and hCG: Validation of the Analytical Method and Application in the Diagnosis of Central Nervous System Germ Cell Tumors |
| title_fullStr | Cerebrospinal Fluid Levels of AFP and hCG: Validation of the Analytical Method and Application in the Diagnosis of Central Nervous System Germ Cell Tumors |
| title_full_unstemmed | Cerebrospinal Fluid Levels of AFP and hCG: Validation of the Analytical Method and Application in the Diagnosis of Central Nervous System Germ Cell Tumors |
| title_short | Cerebrospinal Fluid Levels of AFP and hCG: Validation of the Analytical Method and Application in the Diagnosis of Central Nervous System Germ Cell Tumors |
| title_sort | cerebrospinal fluid levels of afp and hcg validation of the analytical method and application in the diagnosis of central nervous system germ cell tumors |
| topic | AFP hCG cerebrospinal fluid germ cell tumors central nervous system tumors |
| url | https://www.mdpi.com/2075-4418/11/11/1980 |
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