Polystyrene Microsphere-Based Immunochromatographic Assay for Detection of Aflatoxin B<sub>1</sub> in Maize
Aflatoxin B<sub>1</sub> (AFB<sub>1</sub>), a mycotoxin, is hepatotoxic, carcinogenic, and nephrotoxic in humans and animals, and contaminate a wide range of maize. In this study, an immunochromatographic assay (ICA) based on polystyrene microspheres (PMs) was developed for se...
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MDPI AG
2021-06-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/11/6/200 |
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| author | Jin Wang Xiangmei Li Xing Shen Ang Zhang Jinxiu Liu Hongtao Lei |
| author_facet | Jin Wang Xiangmei Li Xing Shen Ang Zhang Jinxiu Liu Hongtao Lei |
| author_sort | Jin Wang |
| collection | DOAJ |
| description | Aflatoxin B<sub>1</sub> (AFB<sub>1</sub>), a mycotoxin, is hepatotoxic, carcinogenic, and nephrotoxic in humans and animals, and contaminate a wide range of maize. In this study, an immunochromatographic assay (ICA) based on polystyrene microspheres (PMs) was developed for sensitive and quantitative detection of AFB<sub>1</sub> in maize. The amounts of PMs, the condition for activating carboxyl groups of PMs, the amount of monoclonal antibody (mAb), and the volume of the immune probe were optimized to enhance the performance PMs-ICA for point-of-care testing of AFB<sub>1</sub> in maize. The PMs-ICA showed the cut-off value of 1 ng/mL in phosphate buffer (PB) and 6 µg/kg in maize samples, respectively. The quantitative limit of detection (qLOD) was 0.27 and 1.43 µg/kg in PB and maize samples, respectively. The accuracy and precision of the PMs-ICA were evaluated by analysis of spiked maize samples with recoveries of 96.0% to 107.6% with coefficients of variation below 10%. In addition, the reliability of PMs-ICA was confirmed by the liquid chromatography-tandem mass spectrometry method. The results indicated that the PMs-ICA could be used as a sensitive, simple, rapid point-of-care testing of AFB<sub>1</sub> in maize. |
| first_indexed | 2024-03-10T10:15:08Z |
| format | Article |
| id | doaj.art-eaada3959a6c4d0489574ec3b77fcc54 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-10T10:15:08Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-eaada3959a6c4d0489574ec3b77fcc542023-11-22T00:54:26ZengMDPI AGBiosensors2079-63742021-06-0111620010.3390/bios11060200Polystyrene Microsphere-Based Immunochromatographic Assay for Detection of Aflatoxin B<sub>1</sub> in MaizeJin Wang0Xiangmei Li1Xing Shen2Ang Zhang3Jinxiu Liu4Hongtao Lei5Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642, ChinaTechnology Center of Qinhuangdao Customs, Qinhuangdao 066004, ChinaGuangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642, ChinaAflatoxin B<sub>1</sub> (AFB<sub>1</sub>), a mycotoxin, is hepatotoxic, carcinogenic, and nephrotoxic in humans and animals, and contaminate a wide range of maize. In this study, an immunochromatographic assay (ICA) based on polystyrene microspheres (PMs) was developed for sensitive and quantitative detection of AFB<sub>1</sub> in maize. The amounts of PMs, the condition for activating carboxyl groups of PMs, the amount of monoclonal antibody (mAb), and the volume of the immune probe were optimized to enhance the performance PMs-ICA for point-of-care testing of AFB<sub>1</sub> in maize. The PMs-ICA showed the cut-off value of 1 ng/mL in phosphate buffer (PB) and 6 µg/kg in maize samples, respectively. The quantitative limit of detection (qLOD) was 0.27 and 1.43 µg/kg in PB and maize samples, respectively. The accuracy and precision of the PMs-ICA were evaluated by analysis of spiked maize samples with recoveries of 96.0% to 107.6% with coefficients of variation below 10%. In addition, the reliability of PMs-ICA was confirmed by the liquid chromatography-tandem mass spectrometry method. The results indicated that the PMs-ICA could be used as a sensitive, simple, rapid point-of-care testing of AFB<sub>1</sub> in maize.https://www.mdpi.com/2079-6374/11/6/200polystyrene microspheresimmunochromatographic assayaflatoxin B<sub>1</sub>maize |
| spellingShingle | Jin Wang Xiangmei Li Xing Shen Ang Zhang Jinxiu Liu Hongtao Lei Polystyrene Microsphere-Based Immunochromatographic Assay for Detection of Aflatoxin B<sub>1</sub> in Maize Biosensors polystyrene microspheres immunochromatographic assay aflatoxin B<sub>1</sub> maize |
| title | Polystyrene Microsphere-Based Immunochromatographic Assay for Detection of Aflatoxin B<sub>1</sub> in Maize |
| title_full | Polystyrene Microsphere-Based Immunochromatographic Assay for Detection of Aflatoxin B<sub>1</sub> in Maize |
| title_fullStr | Polystyrene Microsphere-Based Immunochromatographic Assay for Detection of Aflatoxin B<sub>1</sub> in Maize |
| title_full_unstemmed | Polystyrene Microsphere-Based Immunochromatographic Assay for Detection of Aflatoxin B<sub>1</sub> in Maize |
| title_short | Polystyrene Microsphere-Based Immunochromatographic Assay for Detection of Aflatoxin B<sub>1</sub> in Maize |
| title_sort | polystyrene microsphere based immunochromatographic assay for detection of aflatoxin b sub 1 sub in maize |
| topic | polystyrene microspheres immunochromatographic assay aflatoxin B<sub>1</sub> maize |
| url | https://www.mdpi.com/2079-6374/11/6/200 |
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