Molecular characterization of Echinococcus granulosusfrom Peru by sequencing of the mitochondrial cytochrome C oxidase subunit 1 gene
Echinococcus granulosus, the etiologic agent of cystic echinococcosis (CE) in humans and other animal species, is distributed worldwide. Ten intra-specific variants, or genotypes (G1-G10), have been defined based on genetic diversity. To determine the genotypes present in endemic areas of Peru, samp...
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2010-09-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000600013 |
| _version_ | 1827840616793374720 |
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| author | Elizabeth Sánchez Omar Cáceres César Náquira David Garcia Gladys Patiño Herrera Silvia Aline C Volotão Octavio Fernandes |
| author_facet | Elizabeth Sánchez Omar Cáceres César Náquira David Garcia Gladys Patiño Herrera Silvia Aline C Volotão Octavio Fernandes |
| author_sort | Elizabeth Sánchez |
| collection | DOAJ |
| description | Echinococcus granulosus, the etiologic agent of cystic echinococcosis (CE) in humans and other animal species, is distributed worldwide. Ten intra-specific variants, or genotypes (G1-G10), have been defined based on genetic diversity. To determine the genotypes present in endemic areas of Peru, samples were collected from cattle (44), sheep (41) and humans (14) from Junín, Puno Huancavelica, Cusco, Arequipa and Ayacucho. DNA was extracted from protoscolex and/or germinal layers derived from 99 E. granulosus isolates and used as templates to amplify the mitochondrial cytochrome C oxidase subunit 1 gene. The resulting polymerase chain reaction products were sequenced and further examined by sequence analysis. All isolates, independent of the host, exhibited the G1 genotype. Phylogenetic analysis showed that three isolates from Ayacucho shared the same cluster with microvariant G1(4). The G1 genotype is considered the most widespread and infectious form of E. granulosusworldwide and our results confirm that the same patterns apply to this country. Therefore, these findings should be taken into consideration in developing prevention strategies and control programs for CE in Peru. |
| first_indexed | 2024-03-12T07:36:51Z |
| format | Article |
| id | doaj.art-eaaf11728db4407d90a14814d5bc4729 |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T07:36:51Z |
| publishDate | 2010-09-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-eaaf11728db4407d90a14814d5bc47292023-09-02T21:28:51ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80602010-09-01105680681010.1590/S0074-02762010000600013Molecular characterization of Echinococcus granulosusfrom Peru by sequencing of the mitochondrial cytochrome C oxidase subunit 1 geneElizabeth SánchezOmar CáceresCésar NáquiraDavid GarciaGladys PatiñoHerrera SilviaAline C VolotãoOctavio FernandesEchinococcus granulosus, the etiologic agent of cystic echinococcosis (CE) in humans and other animal species, is distributed worldwide. Ten intra-specific variants, or genotypes (G1-G10), have been defined based on genetic diversity. To determine the genotypes present in endemic areas of Peru, samples were collected from cattle (44), sheep (41) and humans (14) from Junín, Puno Huancavelica, Cusco, Arequipa and Ayacucho. DNA was extracted from protoscolex and/or germinal layers derived from 99 E. granulosus isolates and used as templates to amplify the mitochondrial cytochrome C oxidase subunit 1 gene. The resulting polymerase chain reaction products were sequenced and further examined by sequence analysis. All isolates, independent of the host, exhibited the G1 genotype. Phylogenetic analysis showed that three isolates from Ayacucho shared the same cluster with microvariant G1(4). The G1 genotype is considered the most widespread and infectious form of E. granulosusworldwide and our results confirm that the same patterns apply to this country. Therefore, these findings should be taken into consideration in developing prevention strategies and control programs for CE in Peru.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000600013Echinococcus granulosuscytochrome C oxidase subunit 1genotypesPeru |
| spellingShingle | Elizabeth Sánchez Omar Cáceres César Náquira David Garcia Gladys Patiño Herrera Silvia Aline C Volotão Octavio Fernandes Molecular characterization of Echinococcus granulosusfrom Peru by sequencing of the mitochondrial cytochrome C oxidase subunit 1 gene Memorias do Instituto Oswaldo Cruz Echinococcus granulosus cytochrome C oxidase subunit 1 genotypes Peru |
| title | Molecular characterization of Echinococcus granulosusfrom Peru by sequencing of the mitochondrial cytochrome C oxidase subunit 1 gene |
| title_full | Molecular characterization of Echinococcus granulosusfrom Peru by sequencing of the mitochondrial cytochrome C oxidase subunit 1 gene |
| title_fullStr | Molecular characterization of Echinococcus granulosusfrom Peru by sequencing of the mitochondrial cytochrome C oxidase subunit 1 gene |
| title_full_unstemmed | Molecular characterization of Echinococcus granulosusfrom Peru by sequencing of the mitochondrial cytochrome C oxidase subunit 1 gene |
| title_short | Molecular characterization of Echinococcus granulosusfrom Peru by sequencing of the mitochondrial cytochrome C oxidase subunit 1 gene |
| title_sort | molecular characterization of echinococcus granulosusfrom peru by sequencing of the mitochondrial cytochrome c oxidase subunit 1 gene |
| topic | Echinococcus granulosus cytochrome C oxidase subunit 1 genotypes Peru |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000600013 |
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