Interferon-γ inhibits group B Streptococcus survival within human endothelial cells
Endothelial dysfunction is a major component of the pathophysiology of septicaemic group B Streptococcus (GBS) infections. Although cytokines have been shown to activate human umbilical vein endothelial cells (HUVECs), the capacity of interferon (IFN)-γ to enhance the microbicidal activity of HUVECs...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2014-11-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000700940&lng=en&tlng=en |
| _version_ | 1797757648028827648 |
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| author | Viviane de Oliveira Freitas Lione Michelle Hanthequeste Bittencourt dos Santos Jessica Silva Santos de Oliveira Ana Luiza Mattos-Guaraldi Prescilla Emy Nagao |
| author_facet | Viviane de Oliveira Freitas Lione Michelle Hanthequeste Bittencourt dos Santos Jessica Silva Santos de Oliveira Ana Luiza Mattos-Guaraldi Prescilla Emy Nagao |
| author_sort | Viviane de Oliveira Freitas Lione |
| collection | DOAJ |
| description | Endothelial dysfunction is a major component of the pathophysiology of septicaemic group B Streptococcus (GBS) infections. Although cytokines have been shown to activate human umbilical vein endothelial cells (HUVECs), the capacity of interferon (IFN)-γ to enhance the microbicidal activity of HUVECs against GBS has not been studied. We report that the viability of intracellular bacteria was reduced in HUVECs activated by IFN-γ. Enhanced fusion of lysosomes with bacteria-containing vacuoles was observed by acid phosphatase and the colocalisation of Rab-5, Rab-7 and lysosomal-associated membrane protein-1 with GBS in IFN-γ-activated HUVECs. IFN-γ resulted in an enhancement of the phagosome maturation process in HUVECs, improving the capacity to control the intracellular survival of GBS. |
| first_indexed | 2024-03-12T18:18:41Z |
| format | Article |
| id | doaj.art-eaaf780d60cc4e56ab6387b15a53ff14 |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T18:18:41Z |
| publishDate | 2014-11-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-eaaf780d60cc4e56ab6387b15a53ff142023-08-02T09:00:10ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-80602014-11-01109794094310.1590/0074-0276140201S0074-02762014000700940Interferon-γ inhibits group B Streptococcus survival within human endothelial cellsViviane de Oliveira Freitas LioneMichelle Hanthequeste Bittencourt dos SantosJessica Silva Santos de OliveiraAna Luiza Mattos-GuaraldiPrescilla Emy NagaoEndothelial dysfunction is a major component of the pathophysiology of septicaemic group B Streptococcus (GBS) infections. Although cytokines have been shown to activate human umbilical vein endothelial cells (HUVECs), the capacity of interferon (IFN)-γ to enhance the microbicidal activity of HUVECs against GBS has not been studied. We report that the viability of intracellular bacteria was reduced in HUVECs activated by IFN-γ. Enhanced fusion of lysosomes with bacteria-containing vacuoles was observed by acid phosphatase and the colocalisation of Rab-5, Rab-7 and lysosomal-associated membrane protein-1 with GBS in IFN-γ-activated HUVECs. IFN-γ resulted in an enhancement of the phagosome maturation process in HUVECs, improving the capacity to control the intracellular survival of GBS.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000700940&lng=en&tlng=engroup B StreptococcusIFN-γsurvival |
| spellingShingle | Viviane de Oliveira Freitas Lione Michelle Hanthequeste Bittencourt dos Santos Jessica Silva Santos de Oliveira Ana Luiza Mattos-Guaraldi Prescilla Emy Nagao Interferon-γ inhibits group B Streptococcus survival within human endothelial cells Memorias do Instituto Oswaldo Cruz group B Streptococcus IFN-γ survival |
| title | Interferon-γ inhibits group B Streptococcus survival within human endothelial cells |
| title_full | Interferon-γ inhibits group B Streptococcus survival within human endothelial cells |
| title_fullStr | Interferon-γ inhibits group B Streptococcus survival within human endothelial cells |
| title_full_unstemmed | Interferon-γ inhibits group B Streptococcus survival within human endothelial cells |
| title_short | Interferon-γ inhibits group B Streptococcus survival within human endothelial cells |
| title_sort | interferon γ inhibits group b streptococcus survival within human endothelial cells |
| topic | group B Streptococcus IFN-γ survival |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000700940&lng=en&tlng=en |
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