Persistent expression of activation markers on Mycobacterium tuberculosis-specific CD4 T cells in smear negative TB patients.
<h4>Background</h4>T cell activation (HLA-DR, CD-38), proliferation (KI-67), and functional (IFN-γ, TNF-α) markers have recently been shown to be useful in predicting and monitoring anti-TB responses in smear positive TB, but previous research did not characterize the activation and prol...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2022-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0271234&type=printable |
| _version_ | 1826545884994732032 |
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| author | Ahmed Esmael Adane Mihret Tamrat Abebe Daniel Mussa Sebsibe Neway Joel Ernst Jyothi Rengarajan Liya Wassie Rawleigh Howe |
| author_facet | Ahmed Esmael Adane Mihret Tamrat Abebe Daniel Mussa Sebsibe Neway Joel Ernst Jyothi Rengarajan Liya Wassie Rawleigh Howe |
| author_sort | Ahmed Esmael |
| collection | DOAJ |
| description | <h4>Background</h4>T cell activation (HLA-DR, CD-38), proliferation (KI-67), and functional (IFN-γ, TNF-α) markers have recently been shown to be useful in predicting and monitoring anti-TB responses in smear positive TB, but previous research did not characterize the activation and proliferation profiles after therapy of smear negative TB.<h4>Methodology</h4>In this study, we used polychromatic flow cytometry to assess selected PPD-specific T cell markers using fresh PBMC of smear negative and positive pulmonary tuberculosis (PTB) patients, recruited from health facilities in Addis Ababa.<h4>Result</h4>Levels of activation (HLA-DR, CD38) and proliferation (Ki-67) among total unstimulated CD4 T cells decreased significantly after therapy, particularly at month 6. Similarly, levels of PPD-specific T cell activation markers (HLA-DR, CD-38) were significantly lower in smear positive PTB patients following treatment, whereas a consistent decline in these markers was less apparent among smear negative PTB patients at the sixth month.<h4>Conclusion</h4>After six months of standard anti-TB therapy, persistent levels of activation of HLA-DR and CD-38 from PPD specific CD4+T cells in this study could indicate that those markers have little value in monitoring and predicting anti-TB treatment response in smear negative pulmonary TB patients in Ethiopian context. |
| first_indexed | 2024-04-11T07:22:38Z |
| format | Article |
| id | doaj.art-eab09cb456114c10b6b0b93a2b8087d9 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2025-03-14T05:24:58Z |
| publishDate | 2022-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-eab09cb456114c10b6b0b93a2b8087d92025-03-06T05:31:32ZengPublic Library of Science (PLoS)PLoS ONE1932-62032022-01-01178e027123410.1371/journal.pone.0271234Persistent expression of activation markers on Mycobacterium tuberculosis-specific CD4 T cells in smear negative TB patients.Ahmed EsmaelAdane MihretTamrat AbebeDaniel MussaSebsibe NewayJoel ErnstJyothi RengarajanLiya WassieRawleigh Howe<h4>Background</h4>T cell activation (HLA-DR, CD-38), proliferation (KI-67), and functional (IFN-γ, TNF-α) markers have recently been shown to be useful in predicting and monitoring anti-TB responses in smear positive TB, but previous research did not characterize the activation and proliferation profiles after therapy of smear negative TB.<h4>Methodology</h4>In this study, we used polychromatic flow cytometry to assess selected PPD-specific T cell markers using fresh PBMC of smear negative and positive pulmonary tuberculosis (PTB) patients, recruited from health facilities in Addis Ababa.<h4>Result</h4>Levels of activation (HLA-DR, CD38) and proliferation (Ki-67) among total unstimulated CD4 T cells decreased significantly after therapy, particularly at month 6. Similarly, levels of PPD-specific T cell activation markers (HLA-DR, CD-38) were significantly lower in smear positive PTB patients following treatment, whereas a consistent decline in these markers was less apparent among smear negative PTB patients at the sixth month.<h4>Conclusion</h4>After six months of standard anti-TB therapy, persistent levels of activation of HLA-DR and CD-38 from PPD specific CD4+T cells in this study could indicate that those markers have little value in monitoring and predicting anti-TB treatment response in smear negative pulmonary TB patients in Ethiopian context.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0271234&type=printable |
| spellingShingle | Ahmed Esmael Adane Mihret Tamrat Abebe Daniel Mussa Sebsibe Neway Joel Ernst Jyothi Rengarajan Liya Wassie Rawleigh Howe Persistent expression of activation markers on Mycobacterium tuberculosis-specific CD4 T cells in smear negative TB patients. PLoS ONE |
| title | Persistent expression of activation markers on Mycobacterium tuberculosis-specific CD4 T cells in smear negative TB patients. |
| title_full | Persistent expression of activation markers on Mycobacterium tuberculosis-specific CD4 T cells in smear negative TB patients. |
| title_fullStr | Persistent expression of activation markers on Mycobacterium tuberculosis-specific CD4 T cells in smear negative TB patients. |
| title_full_unstemmed | Persistent expression of activation markers on Mycobacterium tuberculosis-specific CD4 T cells in smear negative TB patients. |
| title_short | Persistent expression of activation markers on Mycobacterium tuberculosis-specific CD4 T cells in smear negative TB patients. |
| title_sort | persistent expression of activation markers on mycobacterium tuberculosis specific cd4 t cells in smear negative tb patients |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0271234&type=printable |
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