First report of Berkeleyomyces basicola (synonymous: Thielaviopsis basicola) on roots of sweet potato (Ipomoea batatas (L.) Lam) in Argentina
Symptomatic sweet potato cv Arapey INIA samples were collected from a commercial production field in Colonia Molina, Guaymallén department, Mendoza province, Argentina. They showed dark rounded lesions, sometimes coalescing with white granular mycelium. Fungus was obtained from symptomatic sweet...
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| Format: | Article |
| Language: | English |
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Facultad de Ciencias Agrarias. Universidad Nacional de Cuyo
2021-07-01
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| Series: | Revista de la Facultad de Ciencias Agrarias |
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| Online Access: | https://revistas.uncu.edu.ar/ojs/index.php/RFCA/article/view/4898 |
| _version_ | 1797756055444258816 |
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| author | Julia A. Martino Liliana del Valle Di Feo Mauro Paccioretti Clara Adriana Contardi Miguel A. Sanchez Leandro I. Ortega Silvina E. Pastor |
| author_facet | Julia A. Martino Liliana del Valle Di Feo Mauro Paccioretti Clara Adriana Contardi Miguel A. Sanchez Leandro I. Ortega Silvina E. Pastor |
| author_sort | Julia A. Martino |
| collection | DOAJ |
| description |
Symptomatic sweet potato cv Arapey INIA samples were collected from a commercial production field in Colonia Molina, Guaymallén department, Mendoza province, Argentina. They showed dark rounded lesions, sometimes coalescing with white granular mycelium. Fungus was obtained from symptomatic sweet potatoes, which represented the generalized infection that affected the crop. They were seeded in PDA with streptomycin sulfate and incubated for seven days at 21°C, alternating white/black (UV400nm) light. Observations with an optical microscope revealed the presence of hyaline, not septated, cylindrical endoconidia with rounded ends. They were 8-16 μm length and 4–6 μm width. Phialides were 43-46 μm length, rounded bases (7-9 μm width) and tapering to the neck´s tip (4-6 μm width). Brown chlamydospores (aleuriospores), 9-13 μm length and 8-12 μm width, in chains of 2-8 spores were observed. For molecular identification, total genomic DNA was extracted. ITS fragment of 565 pb was amplified using ITS5/ITS4 primers and sequenced. The sequence indicated 99% identity with Berkeleyomyces basicola (synonymous: Thielaviopsis basicola). This was deposited in GenBank as (KX580957) (CBS: C430.74, Gen Bank accession number AF275482.1). This is the first report of B. basicola in sweet potato in Argentina, a potential threat to storage root yields.
Highlights:
Sweet potato black root rot, new disease in Argentina.
First report of Berkeleyomyces basicola causing black root rot on sweet potato in Mendoza, Argentina.
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| first_indexed | 2024-03-12T17:55:59Z |
| format | Article |
| id | doaj.art-eab1b9b646bf46d5a78a381c22ccdbbe |
| institution | Directory Open Access Journal |
| issn | 0370-4661 1853-8665 |
| language | English |
| last_indexed | 2024-03-12T17:55:59Z |
| publishDate | 2021-07-01 |
| publisher | Facultad de Ciencias Agrarias. Universidad Nacional de Cuyo |
| record_format | Article |
| series | Revista de la Facultad de Ciencias Agrarias |
| spelling | doaj.art-eab1b9b646bf46d5a78a381c22ccdbbe2023-08-02T17:52:15ZengFacultad de Ciencias Agrarias. Universidad Nacional de CuyoRevista de la Facultad de Ciencias Agrarias0370-46611853-86652021-07-0153110.48162/rev.39.027First report of Berkeleyomyces basicola (synonymous: Thielaviopsis basicola) on roots of sweet potato (Ipomoea batatas (L.) Lam) in ArgentinaJulia A. Martino0Liliana del Valle Di Feo1Mauro Paccioretti2Clara Adriana Contardi3Miguel A. Sanchez4Leandro I. Ortega5Silvina E. Pastor6Instituto Nacional de Tecnología Agropecuaria. Instituto de Patología Vegetal (Argentina). 11 de Septiembre 4755 (ex-camino 60 cuadras km 5,5). X5020ICA. Córdoba. ArgentinaInstituto Nacional de Tecnología Agropecuaria. Instituto de Patología Vegetal (Argentina). 11 de Septiembre 4755 (ex-camino 60 cuadras km 5,5). X5020ICA. Córdoba. Argentina.Instituto Nacional de Tecnología Agropecuaria. Instituto de Patología Vegetal (Argentina). 11 de Septiembre 4755 (ex-camino 60 cuadras km 5,5). X5020ICA. Córdoba. ArgentinaInstituto Nacional de Tecnología Agropecuaria. Agencia de Extensión Rural Luján de Cuyo. Mendoza. ArgentinaConsejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (Argentina). 11 de Septiembre 4755 (ex-camino 60 cuadras km 5,5). X5020ICA. Córdoba. ArgentinaInstituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales (Argentina) 11 de Septiembre 4755 (ex-camino 60 cuadras km 5,5). X5020ICA. Córdoba. ArgentinaInstituto Nacional de Tecnología Agropecuaria. Instituto de Patología Vegetal (Argentina). 11 de Septiembre 4755 (ex-camino 60 cuadras km 5,5). X5020ICA. Córdoba. Argentina. Symptomatic sweet potato cv Arapey INIA samples were collected from a commercial production field in Colonia Molina, Guaymallén department, Mendoza province, Argentina. They showed dark rounded lesions, sometimes coalescing with white granular mycelium. Fungus was obtained from symptomatic sweet potatoes, which represented the generalized infection that affected the crop. They were seeded in PDA with streptomycin sulfate and incubated for seven days at 21°C, alternating white/black (UV400nm) light. Observations with an optical microscope revealed the presence of hyaline, not septated, cylindrical endoconidia with rounded ends. They were 8-16 μm length and 4–6 μm width. Phialides were 43-46 μm length, rounded bases (7-9 μm width) and tapering to the neck´s tip (4-6 μm width). Brown chlamydospores (aleuriospores), 9-13 μm length and 8-12 μm width, in chains of 2-8 spores were observed. For molecular identification, total genomic DNA was extracted. ITS fragment of 565 pb was amplified using ITS5/ITS4 primers and sequenced. The sequence indicated 99% identity with Berkeleyomyces basicola (synonymous: Thielaviopsis basicola). This was deposited in GenBank as (KX580957) (CBS: C430.74, Gen Bank accession number AF275482.1). This is the first report of B. basicola in sweet potato in Argentina, a potential threat to storage root yields. Highlights: Sweet potato black root rot, new disease in Argentina. First report of Berkeleyomyces basicola causing black root rot on sweet potato in Mendoza, Argentina. https://revistas.uncu.edu.ar/ojs/index.php/RFCA/article/view/4898Ipomoea batatasroot rotChalara eleganspotatosweet potato fungal disease |
| spellingShingle | Julia A. Martino Liliana del Valle Di Feo Mauro Paccioretti Clara Adriana Contardi Miguel A. Sanchez Leandro I. Ortega Silvina E. Pastor First report of Berkeleyomyces basicola (synonymous: Thielaviopsis basicola) on roots of sweet potato (Ipomoea batatas (L.) Lam) in Argentina Revista de la Facultad de Ciencias Agrarias Ipomoea batatas root rot Chalara elegans potato sweet potato fungal disease |
| title | First report of Berkeleyomyces basicola (synonymous: Thielaviopsis basicola) on roots of sweet potato (Ipomoea batatas (L.) Lam) in Argentina |
| title_full | First report of Berkeleyomyces basicola (synonymous: Thielaviopsis basicola) on roots of sweet potato (Ipomoea batatas (L.) Lam) in Argentina |
| title_fullStr | First report of Berkeleyomyces basicola (synonymous: Thielaviopsis basicola) on roots of sweet potato (Ipomoea batatas (L.) Lam) in Argentina |
| title_full_unstemmed | First report of Berkeleyomyces basicola (synonymous: Thielaviopsis basicola) on roots of sweet potato (Ipomoea batatas (L.) Lam) in Argentina |
| title_short | First report of Berkeleyomyces basicola (synonymous: Thielaviopsis basicola) on roots of sweet potato (Ipomoea batatas (L.) Lam) in Argentina |
| title_sort | first report of berkeleyomyces basicola synonymous thielaviopsis basicola on roots of sweet potato ipomoea batatas l lam in argentina |
| topic | Ipomoea batatas root rot Chalara elegans potato sweet potato fungal disease |
| url | https://revistas.uncu.edu.ar/ojs/index.php/RFCA/article/view/4898 |
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