Novel targets in rectal cancer by considering lncRNA–miRNA–mRNA network in response to Lactobacillus acidophilus consumption: a randomized clinical trial
Abstract We aimed to explore the lncRNA–miR–mRNA network in response to Lactobacillus acidophilus (L. acidophilus) consumption in rectal cancer patients. The candidate miRs were first taken from the GEO and TCGA databases. We constructed the lncRNA–miR–mRNA network using the high-throughput sequenci...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-06-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-13297-9 |
| _version_ | 1817968854715858944 |
|---|---|
| author | Zohreh Khodaii Mahboobeh Mehrabani Natanzi Solmaz Khalighfard Maziar Ghandian Zanjan Maryam Gharghi Vahid Khori Taghi Amiriani Monireh Rahimkhani Ali Mohammad Alizadeh |
| author_facet | Zohreh Khodaii Mahboobeh Mehrabani Natanzi Solmaz Khalighfard Maziar Ghandian Zanjan Maryam Gharghi Vahid Khori Taghi Amiriani Monireh Rahimkhani Ali Mohammad Alizadeh |
| author_sort | Zohreh Khodaii |
| collection | DOAJ |
| description | Abstract We aimed to explore the lncRNA–miR–mRNA network in response to Lactobacillus acidophilus (L. acidophilus) consumption in rectal cancer patients. The candidate miRs were first taken from the GEO and TCGA databases. We constructed the lncRNA–miR–mRNA network using the high-throughput sequencing data. At last, we created a heatmap based on the experimental data to show the possible correlation of the selected targets. The expression levels of selected targets were measured in the samples of 107 rectal cancer patients undergoing placebo and probiotic consumption and 10 noncancerous subjects using Real-Time PCR. Our analysis revealed a group of differentially expressed 12 miRs and 11 lncRNAs, and 12 genes in rectal cancer patients. A significant expression increase of the selected tumor suppressor miRs, lncRNAs, and genes and a substantial expression decrease of the selected oncomiRs, onco-lncRNAs, and oncogenes were obtained after the probiotic consumption compared to the placebo group. There is a strong correlation between some network components, including miR-133b and IGF1 gene, miR-548ac and MSH2 gene, and miR-21 and SMAD4 gene. In rectal cancer patients, L. acidophilus consumption was associated with improved expression of the lncRNA–miR–mRNA network, which may provide novel monitoring and therapeutic approaches. |
| first_indexed | 2024-04-13T20:14:06Z |
| format | Article |
| id | doaj.art-eab24b0fc0744100a31fec5506ad7ab2 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-13T20:14:06Z |
| publishDate | 2022-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-eab24b0fc0744100a31fec5506ad7ab22022-12-22T02:31:46ZengNature PortfolioScientific Reports2045-23222022-06-0112111810.1038/s41598-022-13297-9Novel targets in rectal cancer by considering lncRNA–miRNA–mRNA network in response to Lactobacillus acidophilus consumption: a randomized clinical trialZohreh Khodaii0Mahboobeh Mehrabani Natanzi1Solmaz Khalighfard2Maziar Ghandian Zanjan3Maryam Gharghi4Vahid Khori5Taghi Amiriani6Monireh Rahimkhani7Ali Mohammad Alizadeh8Dietary Supplements and Probiotic Research Center, Alborz University of Medical SciencesEvidence-Based Phytotherapy and Complementary Medicine Research Center, Alborz University of Medical SciencesDepartment of Biology, Science and Research Branch, Islamic Azad UniversityIschemic Disorders Research Center, Golestan University of Medical SciencesIschemic Disorders Research Center, Golestan University of Medical SciencesIschemic Disorders Research Center, Golestan University of Medical SciencesIschemic Disorders Research Center, Golestan University of Medical SciencesFaculty of Allied Medical Sciences, Tehran University of Medical SciencesCancer Research Center, Cancer Institute, Tehran University of Medical SciencesAbstract We aimed to explore the lncRNA–miR–mRNA network in response to Lactobacillus acidophilus (L. acidophilus) consumption in rectal cancer patients. The candidate miRs were first taken from the GEO and TCGA databases. We constructed the lncRNA–miR–mRNA network using the high-throughput sequencing data. At last, we created a heatmap based on the experimental data to show the possible correlation of the selected targets. The expression levels of selected targets were measured in the samples of 107 rectal cancer patients undergoing placebo and probiotic consumption and 10 noncancerous subjects using Real-Time PCR. Our analysis revealed a group of differentially expressed 12 miRs and 11 lncRNAs, and 12 genes in rectal cancer patients. A significant expression increase of the selected tumor suppressor miRs, lncRNAs, and genes and a substantial expression decrease of the selected oncomiRs, onco-lncRNAs, and oncogenes were obtained after the probiotic consumption compared to the placebo group. There is a strong correlation between some network components, including miR-133b and IGF1 gene, miR-548ac and MSH2 gene, and miR-21 and SMAD4 gene. In rectal cancer patients, L. acidophilus consumption was associated with improved expression of the lncRNA–miR–mRNA network, which may provide novel monitoring and therapeutic approaches.https://doi.org/10.1038/s41598-022-13297-9 |
| spellingShingle | Zohreh Khodaii Mahboobeh Mehrabani Natanzi Solmaz Khalighfard Maziar Ghandian Zanjan Maryam Gharghi Vahid Khori Taghi Amiriani Monireh Rahimkhani Ali Mohammad Alizadeh Novel targets in rectal cancer by considering lncRNA–miRNA–mRNA network in response to Lactobacillus acidophilus consumption: a randomized clinical trial Scientific Reports |
| title | Novel targets in rectal cancer by considering lncRNA–miRNA–mRNA network in response to Lactobacillus acidophilus consumption: a randomized clinical trial |
| title_full | Novel targets in rectal cancer by considering lncRNA–miRNA–mRNA network in response to Lactobacillus acidophilus consumption: a randomized clinical trial |
| title_fullStr | Novel targets in rectal cancer by considering lncRNA–miRNA–mRNA network in response to Lactobacillus acidophilus consumption: a randomized clinical trial |
| title_full_unstemmed | Novel targets in rectal cancer by considering lncRNA–miRNA–mRNA network in response to Lactobacillus acidophilus consumption: a randomized clinical trial |
| title_short | Novel targets in rectal cancer by considering lncRNA–miRNA–mRNA network in response to Lactobacillus acidophilus consumption: a randomized clinical trial |
| title_sort | novel targets in rectal cancer by considering lncrna mirna mrna network in response to lactobacillus acidophilus consumption a randomized clinical trial |
| url | https://doi.org/10.1038/s41598-022-13297-9 |
| work_keys_str_mv | AT zohrehkhodaii noveltargetsinrectalcancerbyconsideringlncrnamirnamrnanetworkinresponsetolactobacillusacidophilusconsumptionarandomizedclinicaltrial AT mahboobehmehrabaninatanzi noveltargetsinrectalcancerbyconsideringlncrnamirnamrnanetworkinresponsetolactobacillusacidophilusconsumptionarandomizedclinicaltrial AT solmazkhalighfard noveltargetsinrectalcancerbyconsideringlncrnamirnamrnanetworkinresponsetolactobacillusacidophilusconsumptionarandomizedclinicaltrial AT maziarghandianzanjan noveltargetsinrectalcancerbyconsideringlncrnamirnamrnanetworkinresponsetolactobacillusacidophilusconsumptionarandomizedclinicaltrial AT maryamgharghi noveltargetsinrectalcancerbyconsideringlncrnamirnamrnanetworkinresponsetolactobacillusacidophilusconsumptionarandomizedclinicaltrial AT vahidkhori noveltargetsinrectalcancerbyconsideringlncrnamirnamrnanetworkinresponsetolactobacillusacidophilusconsumptionarandomizedclinicaltrial AT taghiamiriani noveltargetsinrectalcancerbyconsideringlncrnamirnamrnanetworkinresponsetolactobacillusacidophilusconsumptionarandomizedclinicaltrial AT monirehrahimkhani noveltargetsinrectalcancerbyconsideringlncrnamirnamrnanetworkinresponsetolactobacillusacidophilusconsumptionarandomizedclinicaltrial AT alimohammadalizadeh noveltargetsinrectalcancerbyconsideringlncrnamirnamrnanetworkinresponsetolactobacillusacidophilusconsumptionarandomizedclinicaltrial |