A cohort study on the association of MDM2 SNP309 with lung cancer risk in Bangladeshi population
Background/Aims Bangladesh is a densely populated country with an increased incidence of lung cancer, mostly due to smoking. Therefore, elucidating the association of mouse double minute 2 homolog (MDM2) single nucleotide polymorphism (SNP) 309 (rs2279744) with lung cancer risk from smoking in Bangl...
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| Language: | English |
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The Korean Association of Internal Medicine
2020-05-01
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| Series: | The Korean Journal of Internal Medicine |
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| Online Access: | http://www.kjim.org/upload/pdf/kjim-2018-125.pdf |
| _version_ | 1818355775868764160 |
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| author | Hasan Al Reza Wardatul Jannat Anamika Md. Miraj Kobad Chowdhury Mohammad Golam Mostafa M. Aftab Uddin |
| author_facet | Hasan Al Reza Wardatul Jannat Anamika Md. Miraj Kobad Chowdhury Mohammad Golam Mostafa M. Aftab Uddin |
| author_sort | Hasan Al Reza |
| collection | DOAJ |
| description | Background/Aims Bangladesh is a densely populated country with an increased incidence of lung cancer, mostly due to smoking. Therefore, elucidating the association of mouse double minute 2 homolog (MDM2) single nucleotide polymorphism (SNP) 309 (rs2279744) with lung cancer risk from smoking in Bangladeshi population has become necessary. Methods DNA was extracted from blood samples of 126 lung cancer patient and 133 healthy controls. The MDM2 SNP309 was genotyped by polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP), using the restriction enzymes MspA1I. Logistic regression was then carried out to calculate odds ratios (ORs) and 95% confidence intervals (CIs) to estimate the risk of lung cancer. A meta-analysis of SNP309 was also carried out on 12,758 control subjects and 11,638 patient subjects. Results In multivariate logistic regression, significantly increased risk of lung cancer was observed for MDM2 SNP309 in the dominant model (TG + GG vs. TT: OR, 2.13; 95% CI, 1.29 to 3.53). Stratification analysis revealed that age, sex, obesity, and smoking also increases the risk of lung cancer when carrying the MDM2 SNP309. Our meta-analysis revealed that MDM2 SNP309 was considerably associated with lung cancer in Asian populations (TG + GG vs. TT: OR, 1.32; 95% CI , 1.12 to 1.56; p = 0.019 for heterogeneity). Conclusions The MDM2 SNP309 was associated with high risk of lung cancer in Bangladeshi and Asian population, particularly with increased age, smoking, and body mass index. |
| first_indexed | 2024-12-13T19:46:41Z |
| format | Article |
| id | doaj.art-eabae63d4d5e4e469e434c47519014dc |
| institution | Directory Open Access Journal |
| issn | 1226-3303 2005-6648 |
| language | English |
| last_indexed | 2024-12-13T19:46:41Z |
| publishDate | 2020-05-01 |
| publisher | The Korean Association of Internal Medicine |
| record_format | Article |
| series | The Korean Journal of Internal Medicine |
| spelling | doaj.art-eabae63d4d5e4e469e434c47519014dc2022-12-21T23:33:32ZengThe Korean Association of Internal MedicineThe Korean Journal of Internal Medicine1226-33032005-66482020-05-0135367268110.3904/kjim.2018.125170372A cohort study on the association of MDM2 SNP309 with lung cancer risk in Bangladeshi populationHasan Al Reza0Wardatul Jannat Anamika1Md. Miraj Kobad Chowdhury2Mohammad Golam Mostafa3M. Aftab Uddin4 Department of Genetic Engineering and Biotechnology, University of Dhaka, Dhaka, Bangladesh Department of Genetic Engineering and Biotechnology, University of Dhaka, Dhaka, Bangladesh Department of Genetic Engineering and Biotechnology, University of Dhaka, Dhaka, Bangladesh Department of Histopathology, National Institute of Cancer Research and Hospital, Dhaka, Bangladesh Department of Genetic Engineering and Biotechnology, University of Dhaka, Dhaka, BangladeshBackground/Aims Bangladesh is a densely populated country with an increased incidence of lung cancer, mostly due to smoking. Therefore, elucidating the association of mouse double minute 2 homolog (MDM2) single nucleotide polymorphism (SNP) 309 (rs2279744) with lung cancer risk from smoking in Bangladeshi population has become necessary. Methods DNA was extracted from blood samples of 126 lung cancer patient and 133 healthy controls. The MDM2 SNP309 was genotyped by polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP), using the restriction enzymes MspA1I. Logistic regression was then carried out to calculate odds ratios (ORs) and 95% confidence intervals (CIs) to estimate the risk of lung cancer. A meta-analysis of SNP309 was also carried out on 12,758 control subjects and 11,638 patient subjects. Results In multivariate logistic regression, significantly increased risk of lung cancer was observed for MDM2 SNP309 in the dominant model (TG + GG vs. TT: OR, 2.13; 95% CI, 1.29 to 3.53). Stratification analysis revealed that age, sex, obesity, and smoking also increases the risk of lung cancer when carrying the MDM2 SNP309. Our meta-analysis revealed that MDM2 SNP309 was considerably associated with lung cancer in Asian populations (TG + GG vs. TT: OR, 1.32; 95% CI , 1.12 to 1.56; p = 0.019 for heterogeneity). Conclusions The MDM2 SNP309 was associated with high risk of lung cancer in Bangladeshi and Asian population, particularly with increased age, smoking, and body mass index.http://www.kjim.org/upload/pdf/kjim-2018-125.pdflung neoplasmsmdm2 genemdm2 snp309meta-analysis |
| spellingShingle | Hasan Al Reza Wardatul Jannat Anamika Md. Miraj Kobad Chowdhury Mohammad Golam Mostafa M. Aftab Uddin A cohort study on the association of MDM2 SNP309 with lung cancer risk in Bangladeshi population The Korean Journal of Internal Medicine lung neoplasms mdm2 gene mdm2 snp309 meta-analysis |
| title | A cohort study on the association of MDM2 SNP309 with lung cancer risk in Bangladeshi population |
| title_full | A cohort study on the association of MDM2 SNP309 with lung cancer risk in Bangladeshi population |
| title_fullStr | A cohort study on the association of MDM2 SNP309 with lung cancer risk in Bangladeshi population |
| title_full_unstemmed | A cohort study on the association of MDM2 SNP309 with lung cancer risk in Bangladeshi population |
| title_short | A cohort study on the association of MDM2 SNP309 with lung cancer risk in Bangladeshi population |
| title_sort | cohort study on the association of mdm2 snp309 with lung cancer risk in bangladeshi population |
| topic | lung neoplasms mdm2 gene mdm2 snp309 meta-analysis |
| url | http://www.kjim.org/upload/pdf/kjim-2018-125.pdf |
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