STABILIZATION OF MICROBIAL ENZYMATIC PREPARATIONS USED IN FEED INDUSTRY
The enzymatic compound with protease activity obtained by Bacillus licheniformis CM1T 1.33 cells fermentation was entrapped in sol-gel matrixes, using TEOS as precursor. The sol-gel technique is a soft method for silica glass synthesis at room temperature. In silica gels, the enzymes maintain their...
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| Format: | Article |
| Language: | English |
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Agroprint Timisoara
2023-11-01
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| Series: | Scientific Papers Animal Science and Biotechnologies |
| Subjects: | |
| Online Access: | https://spasb.ro/index.php/public_html/article/view/1927 |
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| author | MONICA DRAGOMIRESCU T. VINTILĂ BEATRICE VLAD-OROS GABRIELA PREDA |
| author_facet | MONICA DRAGOMIRESCU T. VINTILĂ BEATRICE VLAD-OROS GABRIELA PREDA |
| author_sort | MONICA DRAGOMIRESCU |
| collection | DOAJ |
| description | The enzymatic compound with protease activity obtained by Bacillus licheniformis
CM1T 1.33 cells fermentation was entrapped in sol-gel matrixes, using TEOS as
precursor. The sol-gel technique is a soft method for silica glass synthesis at room
temperature. In silica gels, the enzymes maintain their catalytic activity for a longer
period o f time and present high stability at environmental factors. Bacillus
licheniformis CMIT 1.33 enzyme was also immobilized by entrapment in silica gels
deposited on a ceramic support. The optimal temperature and pH o f the native and.
immobilized enzyme did not vary significantly. At temperature and pH values lower
than the optimum, the relative activities have been higher for the immobilized
compound. The immobilization o f the enzymatic compound increased the stability.
The enzymatic compounds with protease activity obtained by silica gels entrapment
and entrapment/deposition on ceramic support can be used as forage additive. |
| first_indexed | 2024-04-24T13:42:17Z |
| format | Article |
| id | doaj.art-eabb44be8111433aa0d61acebdd54c86 |
| institution | Directory Open Access Journal |
| issn | 1841-9364 2344-4576 |
| language | English |
| last_indexed | 2024-04-24T13:42:17Z |
| publishDate | 2023-11-01 |
| publisher | Agroprint Timisoara |
| record_format | Article |
| series | Scientific Papers Animal Science and Biotechnologies |
| spelling | doaj.art-eabb44be8111433aa0d61acebdd54c862024-04-04T07:47:43ZengAgroprint TimisoaraScientific Papers Animal Science and Biotechnologies1841-93642344-45762023-11-0141169691927STABILIZATION OF MICROBIAL ENZYMATIC PREPARATIONS USED IN FEED INDUSTRYMONICA DRAGOMIRESCU0T. VINTILĂ1BEATRICE VLAD-OROS2GABRIELA PREDA3Faculty o f Animal Sciences and Biotechnologies, Timişoara, RomâniaFaculty o f Animal Sciences and Biotechnologies, Timişoara, RomâniaFaculty o f Chemistry-Biology-Geography, West University o f Timişoara, RomâniaFaculty o f Chemistry-Biology-Geography, West University o f Timişoara, RomâniaThe enzymatic compound with protease activity obtained by Bacillus licheniformis CM1T 1.33 cells fermentation was entrapped in sol-gel matrixes, using TEOS as precursor. The sol-gel technique is a soft method for silica glass synthesis at room temperature. In silica gels, the enzymes maintain their catalytic activity for a longer period o f time and present high stability at environmental factors. Bacillus licheniformis CMIT 1.33 enzyme was also immobilized by entrapment in silica gels deposited on a ceramic support. The optimal temperature and pH o f the native and. immobilized enzyme did not vary significantly. At temperature and pH values lower than the optimum, the relative activities have been higher for the immobilized compound. The immobilization o f the enzymatic compound increased the stability. The enzymatic compounds with protease activity obtained by silica gels entrapment and entrapment/deposition on ceramic support can be used as forage additive.https://spasb.ro/index.php/public_html/article/view/1927feed enzymesmicrobal proteasesbacillus licheniformisimmobilizationsol-gelteos |
| spellingShingle | MONICA DRAGOMIRESCU T. VINTILĂ BEATRICE VLAD-OROS GABRIELA PREDA STABILIZATION OF MICROBIAL ENZYMATIC PREPARATIONS USED IN FEED INDUSTRY Scientific Papers Animal Science and Biotechnologies feed enzymes microbal proteases bacillus licheniformis immobilization sol-gel teos |
| title | STABILIZATION OF MICROBIAL ENZYMATIC PREPARATIONS USED IN FEED INDUSTRY |
| title_full | STABILIZATION OF MICROBIAL ENZYMATIC PREPARATIONS USED IN FEED INDUSTRY |
| title_fullStr | STABILIZATION OF MICROBIAL ENZYMATIC PREPARATIONS USED IN FEED INDUSTRY |
| title_full_unstemmed | STABILIZATION OF MICROBIAL ENZYMATIC PREPARATIONS USED IN FEED INDUSTRY |
| title_short | STABILIZATION OF MICROBIAL ENZYMATIC PREPARATIONS USED IN FEED INDUSTRY |
| title_sort | stabilization of microbial enzymatic preparations used in feed industry |
| topic | feed enzymes microbal proteases bacillus licheniformis immobilization sol-gel teos |
| url | https://spasb.ro/index.php/public_html/article/view/1927 |
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