Anticancer effects of punicalagin and 5-fluorouracil on laryngeal squamous cell carcinoma: an <i>in vitro</i> study
The purpose of this study was to assess the apoptotic effects of punicalagin alone and in combination with 5-fluorouracil (5-FU) on laryngeal squamous cell carcinoma (Hep-2) cell line. Hep-2 cells were cultured and divided into four groups: Group 1 received no therapy and served as control, Group 2...
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| Format: | Article |
| Language: | English |
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PAGEPress Publications
2024-02-01
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| Series: | Italian Journal of Medicine |
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| Online Access: | https://www.italjmed.org/ijm/article/view/1699 |
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| author | Amany M. Taha Eman Abd-Elshafy Asmaa M. Khalifa Najla Dar-Odeh Shadia Elsayed Daniah S. Alharkan Aishah Alhodhodi Ahmed Mohammed Sapri Yassmeen SalahEldin Ragheb Gihan A. Balbola |
| author_facet | Amany M. Taha Eman Abd-Elshafy Asmaa M. Khalifa Najla Dar-Odeh Shadia Elsayed Daniah S. Alharkan Aishah Alhodhodi Ahmed Mohammed Sapri Yassmeen SalahEldin Ragheb Gihan A. Balbola |
| author_sort | Amany M. Taha |
| collection | DOAJ |
| description |
The purpose of this study was to assess the apoptotic effects of punicalagin alone and in combination with 5-fluorouracil (5-FU) on laryngeal squamous cell carcinoma (Hep-2) cell line. Hep-2 cells were cultured and divided into four groups: Group 1 received no therapy and served as control, Group 2 received 5-FU only, Group 3 received punicalagin only, and Group 4 received a combination of 5-FU and punicalagin. After 48 hours of incubation, cellular changes were examined under an inverted microscope. The methyl thiazolyl tetrazolium assay, caspase-3 gene level, and vascular endothelial growth factor (VEGF) level were assessed. The control group showed the highest mean value of cancer cell proliferation rate (1.595±0.58), followed by the punicalagin group (1.263±0.447), then the 5-FU group (0.827±0.256), while the combination group showed the lowest proliferation rate (0.253±0.111). The combination group showed the highest mean value of caspase-3 concentration (3.177±0.736), followed by the 5-FU group (1.830±0.646), and punicalagin group (0.741±0.302), while the control group showed the lowest mean value (0.359±0.117). Regarding VEGF levels, the control group had a statistically significant higher mean value, followed by the punicalagin and 5-FU groups, and finally, the combination group which showed the lowest value. Punicalagin exerts an anticancer effect through anti-proliferative action and induction of apoptosis on Hep-2 cell line. Combining punicalagin with 5-FU potentiates its anti-proliferative, apoptotic, and anti-angiogenic actions. It, further, helps in mitigating the putative side effects of 5-FU by reducing the dose required for its therapeutic effects.
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| first_indexed | 2024-03-07T22:58:40Z |
| format | Article |
| id | doaj.art-eabb9dae13a843108e88667ef0707e61 |
| institution | Directory Open Access Journal |
| issn | 1877-9344 1877-9352 |
| language | English |
| last_indexed | 2024-03-07T22:58:40Z |
| publishDate | 2024-02-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | Italian Journal of Medicine |
| spelling | doaj.art-eabb9dae13a843108e88667ef0707e612024-02-22T20:42:21ZengPAGEPress PublicationsItalian Journal of Medicine1877-93441877-93522024-02-0118110.4081/itjm.2024.1699Anticancer effects of punicalagin and 5-fluorouracil on laryngeal squamous cell carcinoma: an <i>in vitro</i> studyAmany M. Taha0Eman Abd-Elshafy1Asmaa M. Khalifa2Najla Dar-Odeh3Shadia ElsayedDaniah S. Alharkan4Aishah Alhodhodi5Ahmed Mohammed Sapri6Yassmeen SalahEldin Ragheb7Gihan A. Balbola8Department of Oral and Dental Pathology, Faculty of Dental Medicine for Girls, Al Azhar University, CairoDepartment of Botany and Microbiology, Faculty of Science (Girls), Al Azhar University, CairoDepartment of Botany and Microbiology, Faculty of Science (Girls), Al Azhar University, CairoCollege of Dentistry, Taibah University, Al-Madinah Al-MunawwarahDepartment of Oral and Maxillofacial Diagnostic Sciences, College of Dentistry, Taibah University, Al-Madinah Al-MunawwarahDepartment of Oral and Maxillofacial Diagnostic Sciences, College of Dentistry, Taibah University, Al-Madinah Al-MunawwarahDepartment of Oral and Maxillofacial Surgery, Faculty of Dentistry, Mansoura UniversityDepartment of Clinical Pharmacy, Al-Munirah General Hospital, Ministry of Health, CairoDepartment of Oral and Dental Pathology, Faculty of Dental Medicine for Girls, Al Azhar University, Cairo The purpose of this study was to assess the apoptotic effects of punicalagin alone and in combination with 5-fluorouracil (5-FU) on laryngeal squamous cell carcinoma (Hep-2) cell line. Hep-2 cells were cultured and divided into four groups: Group 1 received no therapy and served as control, Group 2 received 5-FU only, Group 3 received punicalagin only, and Group 4 received a combination of 5-FU and punicalagin. After 48 hours of incubation, cellular changes were examined under an inverted microscope. The methyl thiazolyl tetrazolium assay, caspase-3 gene level, and vascular endothelial growth factor (VEGF) level were assessed. The control group showed the highest mean value of cancer cell proliferation rate (1.595±0.58), followed by the punicalagin group (1.263±0.447), then the 5-FU group (0.827±0.256), while the combination group showed the lowest proliferation rate (0.253±0.111). The combination group showed the highest mean value of caspase-3 concentration (3.177±0.736), followed by the 5-FU group (1.830±0.646), and punicalagin group (0.741±0.302), while the control group showed the lowest mean value (0.359±0.117). Regarding VEGF levels, the control group had a statistically significant higher mean value, followed by the punicalagin and 5-FU groups, and finally, the combination group which showed the lowest value. Punicalagin exerts an anticancer effect through anti-proliferative action and induction of apoptosis on Hep-2 cell line. Combining punicalagin with 5-FU potentiates its anti-proliferative, apoptotic, and anti-angiogenic actions. It, further, helps in mitigating the putative side effects of 5-FU by reducing the dose required for its therapeutic effects. https://www.italjmed.org/ijm/article/view/1699Laryngeal squamous cell carcinomaHep-2 cell linepunicalagincaspase-3vascular endothelial growth factor |
| spellingShingle | Amany M. Taha Eman Abd-Elshafy Asmaa M. Khalifa Najla Dar-Odeh Shadia Elsayed Daniah S. Alharkan Aishah Alhodhodi Ahmed Mohammed Sapri Yassmeen SalahEldin Ragheb Gihan A. Balbola Anticancer effects of punicalagin and 5-fluorouracil on laryngeal squamous cell carcinoma: an <i>in vitro</i> study Italian Journal of Medicine Laryngeal squamous cell carcinoma Hep-2 cell line punicalagin caspase-3 vascular endothelial growth factor |
| title | Anticancer effects of punicalagin and 5-fluorouracil on laryngeal squamous cell carcinoma: an <i>in vitro</i> study |
| title_full | Anticancer effects of punicalagin and 5-fluorouracil on laryngeal squamous cell carcinoma: an <i>in vitro</i> study |
| title_fullStr | Anticancer effects of punicalagin and 5-fluorouracil on laryngeal squamous cell carcinoma: an <i>in vitro</i> study |
| title_full_unstemmed | Anticancer effects of punicalagin and 5-fluorouracil on laryngeal squamous cell carcinoma: an <i>in vitro</i> study |
| title_short | Anticancer effects of punicalagin and 5-fluorouracil on laryngeal squamous cell carcinoma: an <i>in vitro</i> study |
| title_sort | anticancer effects of punicalagin and 5 fluorouracil on laryngeal squamous cell carcinoma an i in vitro i study |
| topic | Laryngeal squamous cell carcinoma Hep-2 cell line punicalagin caspase-3 vascular endothelial growth factor |
| url | https://www.italjmed.org/ijm/article/view/1699 |
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