Expression of Sec61alpha in F9 and P19 teratocarcinoma cells after retinoic acid treatment
Nascent procollagen peptides and other secretory proteins are transported across the endoplasmic reticulum (RE) membrane through a protein-conducting channel called the translocon. Sec61alpha, a multispanning membrane translocon protein, has been implicated as essential for translocation of polypept...
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| Language: | English |
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Instituto Internacional de Ecologia
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| Series: | Brazilian Journal of Biology |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842003000200009&lng=en&tlng=en |
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| author | L. R. Ferreira C. E. E. Velano E. C. Braga C. C. Paula H. Martélli-Junior J. J. Sauk |
| author_facet | L. R. Ferreira C. E. E. Velano E. C. Braga C. C. Paula H. Martélli-Junior J. J. Sauk |
| author_sort | L. R. Ferreira |
| collection | DOAJ |
| description | Nascent procollagen peptides and other secretory proteins are transported across the endoplasmic reticulum (RE) membrane through a protein-conducting channel called the translocon. Sec61alpha, a multispanning membrane translocon protein, has been implicated as essential for translocation of polypeptides chains into the cisterns of the ER. However, it is not known whether Sec61alpha is ubiquitously expressed in collagen producing teratocarcinoma cells. Furthermore, the production, expression, and utilization of Sec61alpha may depend on the cell differentiation stage. Stem cells from many cultured teratocarcinoma cell lines such as F9 and P19 cells are capable of differentiation in response to low retinoic acid concentrations. This differentiation of the tumorigenic stem cells results in tumorigenicity loss. For this study, mouse F9 and P19 teratocarcinoma cells were grown in culture medium treated with or without retinoic acid. Expression of Sec61alpha was determined by reverse trancriptase polimerase chain reaction (RT-PCR). In untreated conditions, F9 cells expressed undetected Sec61alpha amounts. It was also demonstrated that Sec61alpha expression is stimulated in F9 cells after retinoic acid treatment for 72 hours. No changes were found in Sec61alpha expression in P19 cells after retinoic acid treatment. These data indicate that the expression of Sec61alpha is enhanced with retinoic acid induced differentiation of F9 teratocarcinoma cells. |
| first_indexed | 2024-12-11T10:50:26Z |
| format | Article |
| id | doaj.art-eabd473b557a4f7c8cecd40d06800208 |
| institution | Directory Open Access Journal |
| issn | 1678-4375 |
| language | English |
| last_indexed | 2024-12-11T10:50:26Z |
| publisher | Instituto Internacional de Ecologia |
| record_format | Article |
| series | Brazilian Journal of Biology |
| spelling | doaj.art-eabd473b557a4f7c8cecd40d068002082022-12-22T01:10:20ZengInstituto Internacional de EcologiaBrazilian Journal of Biology1678-437563224525210.1590/S1519-69842003000200009S1519-69842003000200009Expression of Sec61alpha in F9 and P19 teratocarcinoma cells after retinoic acid treatmentL. R. Ferreira0C. E. E. Velano1E. C. Braga2C. C. Paula3H. Martélli-Junior4J. J. Sauk5Pontifícia Universidade CatólicaUniversidade José do Rosário VellanoUniversidade José do Rosário VellanoUniversidade José do Rosário VellanoUniversidade Estadual de Montes ClarosUniversity of MarylandNascent procollagen peptides and other secretory proteins are transported across the endoplasmic reticulum (RE) membrane through a protein-conducting channel called the translocon. Sec61alpha, a multispanning membrane translocon protein, has been implicated as essential for translocation of polypeptides chains into the cisterns of the ER. However, it is not known whether Sec61alpha is ubiquitously expressed in collagen producing teratocarcinoma cells. Furthermore, the production, expression, and utilization of Sec61alpha may depend on the cell differentiation stage. Stem cells from many cultured teratocarcinoma cell lines such as F9 and P19 cells are capable of differentiation in response to low retinoic acid concentrations. This differentiation of the tumorigenic stem cells results in tumorigenicity loss. For this study, mouse F9 and P19 teratocarcinoma cells were grown in culture medium treated with or without retinoic acid. Expression of Sec61alpha was determined by reverse trancriptase polimerase chain reaction (RT-PCR). In untreated conditions, F9 cells expressed undetected Sec61alpha amounts. It was also demonstrated that Sec61alpha expression is stimulated in F9 cells after retinoic acid treatment for 72 hours. No changes were found in Sec61alpha expression in P19 cells after retinoic acid treatment. These data indicate that the expression of Sec61alpha is enhanced with retinoic acid induced differentiation of F9 teratocarcinoma cells.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842003000200009&lng=en&tlng=enSec61alphaexpressionF9 teratocarcinoma cellsretinoic acid |
| spellingShingle | L. R. Ferreira C. E. E. Velano E. C. Braga C. C. Paula H. Martélli-Junior J. J. Sauk Expression of Sec61alpha in F9 and P19 teratocarcinoma cells after retinoic acid treatment Brazilian Journal of Biology Sec61alpha expression F9 teratocarcinoma cells retinoic acid |
| title | Expression of Sec61alpha in F9 and P19 teratocarcinoma cells after retinoic acid treatment |
| title_full | Expression of Sec61alpha in F9 and P19 teratocarcinoma cells after retinoic acid treatment |
| title_fullStr | Expression of Sec61alpha in F9 and P19 teratocarcinoma cells after retinoic acid treatment |
| title_full_unstemmed | Expression of Sec61alpha in F9 and P19 teratocarcinoma cells after retinoic acid treatment |
| title_short | Expression of Sec61alpha in F9 and P19 teratocarcinoma cells after retinoic acid treatment |
| title_sort | expression of sec61alpha in f9 and p19 teratocarcinoma cells after retinoic acid treatment |
| topic | Sec61alpha expression F9 teratocarcinoma cells retinoic acid |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842003000200009&lng=en&tlng=en |
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