Untargeted and Targeted Metabolomic Profiling of Preterm Newborns with EarlyOnset Sepsis: A Case-Control Study
Sepsis is a major concern in neonatology, but there are no reliable biomarkers for its early diagnosis. The aim of the study was to compare the metabolic profiles of plasma and urine samples collected at birth from preterm neonates with and without earlyonset sepsis (EOS) to identify metabolic pertu...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-02-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/11/2/115 |
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| author | Veronica Mardegan Giuseppe Giordano Matteo Stocchero Paola Pirillo Gabriele Poloniato Enrica Donadel Sabrina Salvadori Carlo Giaquinto Elena Priante Eugenio Baraldi |
| author_facet | Veronica Mardegan Giuseppe Giordano Matteo Stocchero Paola Pirillo Gabriele Poloniato Enrica Donadel Sabrina Salvadori Carlo Giaquinto Elena Priante Eugenio Baraldi |
| author_sort | Veronica Mardegan |
| collection | DOAJ |
| description | Sepsis is a major concern in neonatology, but there are no reliable biomarkers for its early diagnosis. The aim of the study was to compare the metabolic profiles of plasma and urine samples collected at birth from preterm neonates with and without earlyonset sepsis (EOS) to identify metabolic perturbations that might orient the search for new early biomarkers. All preterm newborns admitted to the neonatal intensive care unit were eligible for this proof-of-concept, prospective case-control study. Infants were enrolled as “cases” if they developed EOS, and as “controls”if they did not. Plasma samples collected at birth and urine samples collected within 24 h of birth underwent untargeted and targeted metabolomic analysis using mass spectrometry coupled with ultra-performance liquid chromatography. Univariate and multivariate statistical analyses were applied. Of 123 eligible newborns, 15 developed EOS. These 15 newborns matched controls for gestational age and weight. Metabolomic analysis revealed evident clustering of the cases versus controls, with the glutathione and tryptophan metabolic pathways markedly disrupted in the former. In conclusion, neonates with EOS had a metabolic profile at birth that clearly distinguished them from those without sepsis, and metabolites of glutathione and tryptophan pathways are promising as new biomarkers of neonatal sepsis. |
| first_indexed | 2024-03-09T00:46:09Z |
| format | Article |
| id | doaj.art-eabec14bab5a4e6ca42321e8fe8e14fe |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-09T00:46:09Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-eabec14bab5a4e6ca42321e8fe8e14fe2023-12-11T17:31:10ZengMDPI AGMetabolites2218-19892021-02-0111211510.3390/metabo11020115Untargeted and Targeted Metabolomic Profiling of Preterm Newborns with EarlyOnset Sepsis: A Case-Control StudyVeronica Mardegan0Giuseppe Giordano1Matteo Stocchero2Paola Pirillo3Gabriele Poloniato4Enrica Donadel5Sabrina Salvadori6Carlo Giaquinto7Elena Priante8Eugenio Baraldi9Women’s and Children’s Health Department, Padua University, 35128 Padua (PD), ItalyWomen’s and Children’s Health Department, Padua University, 35128 Padua (PD), ItalyWomen’s and Children’s Health Department, Padua University, 35128 Padua (PD), ItalyWomen’s and Children’s Health Department, Padua University, 35128 Padua (PD), ItalyWomen’s and Children’s Health Department, Padua University, 35128 Padua (PD), ItalyWomen’s and Children’s Health Department, Padua University, 35128 Padua (PD), ItalyWomen’s and Children’s Health Department, Padua University, 35128 Padua (PD), ItalyWomen’s and Children’s Health Department, Padua University, 35128 Padua (PD), ItalyWomen’s and Children’s Health Department, Padua University, 35128 Padua (PD), ItalyWomen’s and Children’s Health Department, Padua University, 35128 Padua (PD), ItalySepsis is a major concern in neonatology, but there are no reliable biomarkers for its early diagnosis. The aim of the study was to compare the metabolic profiles of plasma and urine samples collected at birth from preterm neonates with and without earlyonset sepsis (EOS) to identify metabolic perturbations that might orient the search for new early biomarkers. All preterm newborns admitted to the neonatal intensive care unit were eligible for this proof-of-concept, prospective case-control study. Infants were enrolled as “cases” if they developed EOS, and as “controls”if they did not. Plasma samples collected at birth and urine samples collected within 24 h of birth underwent untargeted and targeted metabolomic analysis using mass spectrometry coupled with ultra-performance liquid chromatography. Univariate and multivariate statistical analyses were applied. Of 123 eligible newborns, 15 developed EOS. These 15 newborns matched controls for gestational age and weight. Metabolomic analysis revealed evident clustering of the cases versus controls, with the glutathione and tryptophan metabolic pathways markedly disrupted in the former. In conclusion, neonates with EOS had a metabolic profile at birth that clearly distinguished them from those without sepsis, and metabolites of glutathione and tryptophan pathways are promising as new biomarkers of neonatal sepsis.https://www.mdpi.com/2218-1989/11/2/115sepsisearlyonset sepsisinfant/newbornmetabolomicsbiomarker |
| spellingShingle | Veronica Mardegan Giuseppe Giordano Matteo Stocchero Paola Pirillo Gabriele Poloniato Enrica Donadel Sabrina Salvadori Carlo Giaquinto Elena Priante Eugenio Baraldi Untargeted and Targeted Metabolomic Profiling of Preterm Newborns with EarlyOnset Sepsis: A Case-Control Study Metabolites sepsis earlyonset sepsis infant/newborn metabolomics biomarker |
| title | Untargeted and Targeted Metabolomic Profiling of Preterm Newborns with EarlyOnset Sepsis: A Case-Control Study |
| title_full | Untargeted and Targeted Metabolomic Profiling of Preterm Newborns with EarlyOnset Sepsis: A Case-Control Study |
| title_fullStr | Untargeted and Targeted Metabolomic Profiling of Preterm Newborns with EarlyOnset Sepsis: A Case-Control Study |
| title_full_unstemmed | Untargeted and Targeted Metabolomic Profiling of Preterm Newborns with EarlyOnset Sepsis: A Case-Control Study |
| title_short | Untargeted and Targeted Metabolomic Profiling of Preterm Newborns with EarlyOnset Sepsis: A Case-Control Study |
| title_sort | untargeted and targeted metabolomic profiling of preterm newborns with earlyonset sepsis a case control study |
| topic | sepsis earlyonset sepsis infant/newborn metabolomics biomarker |
| url | https://www.mdpi.com/2218-1989/11/2/115 |
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