Rapid screening of complex DNA samples by single-molecule amplification and sequencing.
Microbial cloning makes Sanger sequencing of complex DNA samples possible but is labor intensive. We present a simple, rapid and robust method that enables laboratories without special equipment to perform single-molecule amplicon sequencing, although in a low-throughput manner, from sub-picogram qu...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21625543/?tool=EBI |
| _version_ | 1818721357296304128 |
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| author | Jiaqi Huang Zongli Zheng Anders F Andersson Lars Engstrand Weimin Ye |
| author_facet | Jiaqi Huang Zongli Zheng Anders F Andersson Lars Engstrand Weimin Ye |
| author_sort | Jiaqi Huang |
| collection | DOAJ |
| description | Microbial cloning makes Sanger sequencing of complex DNA samples possible but is labor intensive. We present a simple, rapid and robust method that enables laboratories without special equipment to perform single-molecule amplicon sequencing, although in a low-throughput manner, from sub-picogram quantities of DNA. The method can also be used for quick quality control of next-generation sequencing libraries, as was demonstrated for a metagenomic sample. |
| first_indexed | 2024-12-17T20:37:27Z |
| format | Article |
| id | doaj.art-eac2739637ef4a72a0cdcb799b0ebe40 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-17T20:37:27Z |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-eac2739637ef4a72a0cdcb799b0ebe402022-12-21T21:33:25ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0165e1972310.1371/journal.pone.0019723Rapid screening of complex DNA samples by single-molecule amplification and sequencing.Jiaqi HuangZongli ZhengAnders F AnderssonLars EngstrandWeimin YeMicrobial cloning makes Sanger sequencing of complex DNA samples possible but is labor intensive. We present a simple, rapid and robust method that enables laboratories without special equipment to perform single-molecule amplicon sequencing, although in a low-throughput manner, from sub-picogram quantities of DNA. The method can also be used for quick quality control of next-generation sequencing libraries, as was demonstrated for a metagenomic sample.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21625543/?tool=EBI |
| spellingShingle | Jiaqi Huang Zongli Zheng Anders F Andersson Lars Engstrand Weimin Ye Rapid screening of complex DNA samples by single-molecule amplification and sequencing. PLoS ONE |
| title | Rapid screening of complex DNA samples by single-molecule amplification and sequencing. |
| title_full | Rapid screening of complex DNA samples by single-molecule amplification and sequencing. |
| title_fullStr | Rapid screening of complex DNA samples by single-molecule amplification and sequencing. |
| title_full_unstemmed | Rapid screening of complex DNA samples by single-molecule amplification and sequencing. |
| title_short | Rapid screening of complex DNA samples by single-molecule amplification and sequencing. |
| title_sort | rapid screening of complex dna samples by single molecule amplification and sequencing |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21625543/?tool=EBI |
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