Rapid screening of complex DNA samples by single-molecule amplification and sequencing.

Rapid screening of complex DNA samples by single-molecule amplification and sequencing.

Microbial cloning makes Sanger sequencing of complex DNA samples possible but is labor intensive. We present a simple, rapid and robust method that enables laboratories without special equipment to perform single-molecule amplicon sequencing, although in a low-throughput manner, from sub-picogram qu...

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Bibliographic Details
Main Authors: Jiaqi Huang, Zongli Zheng, Anders F Andersson, Lars Engstrand, Weimin Ye
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2011-01-01
Series:PLoS ONE
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21625543/?tool=EBI

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