Molecular Epidemiology of Azole-Resistant <i>Aspergillus fumigatus</i> in Sawmills of Eastern France by Microsatellite Genotyping
<b>Background:</b> Wood chipping has been described as a potential hotspot for the selection of azole-resistant <i>Aspergillus fumigatus</i> (AR<i>Af</i>). We previously reported AR<i>Af</i> isolates in sawmills (Eastern France), most of which containe...
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MDPI AG
2020-07-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/6/3/120 |
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| author | Audrey Jeanvoine Chloé Godeau Audrey Laboissière Gabriel Reboux Laurence Millon Steffi Rocchi |
| author_facet | Audrey Jeanvoine Chloé Godeau Audrey Laboissière Gabriel Reboux Laurence Millon Steffi Rocchi |
| author_sort | Audrey Jeanvoine |
| collection | DOAJ |
| description | <b>Background:</b> Wood chipping has been described as a potential hotspot for the selection of azole-resistant <i>Aspergillus fumigatus</i> (AR<i>Af</i>). We previously reported AR<i>Af</i> isolates in sawmills (Eastern France), most of which contained the TR<sub>34</sub>/L98H mutation. <b>Methods:</b> To study genotypic relatedness, microsatellite genotyping (short tandem repeat for <i>A. fumigatus</i> (STR<i>Af</i>)) was performed on 41 azole-susceptible <i>A. fumigatus</i> (AS<i>Af</i>) and 23 AR<i>Af</i> isolated from 18 sawmills and two clinical <i>A. fumigatus</i> (sensitive and resistant) isolated from a sinus sample of a woodworker. <b>Results:</b> Fifty-four unique multilocus genotypes (MLGs) were described among the 66 isolates: 13/24 AR<i>Af</i> and 41/42 AS<i>Af.</i> Allelic diversity was higher for AS<i>Af</i> than for AR<i>Af</i>. Among the 24 AR<i>Af</i>, five isolates had their own MLGs. Thirteen AR<i>Af</i> (54%) belonged to the same group, composed of four close MLGs, defined using Bruvo’s distance. Thirty-two of the 42 AS<i>Af</i> (76%) had their own MLGs and could not be grouped with the Bruvo’s distance cutoff used (0.2). <b>Conclusion:</b> Thus, at a regional scale and in the particular environment of the wood industry, common but also different distinct genotypes, even in the same sawmill, were identified. This suggests that the hypothesis of AR<i>Af</i> clonal expansion from a common strain is probably insufficient to explain genotype emergence and distribution. |
| first_indexed | 2024-03-10T18:12:32Z |
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| language | English |
| last_indexed | 2024-03-10T18:12:32Z |
| publishDate | 2020-07-01 |
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| spelling | doaj.art-eac32e6ee82f4ede86ce3c16c67eaede2023-11-20T07:59:16ZengMDPI AGJournal of Fungi2309-608X2020-07-016312010.3390/jof6030120Molecular Epidemiology of Azole-Resistant <i>Aspergillus fumigatus</i> in Sawmills of Eastern France by Microsatellite GenotypingAudrey Jeanvoine0Chloé Godeau1Audrey Laboissière2Gabriel Reboux3Laurence Millon4Steffi Rocchi5Parasitology-Mycology Department, University Hospital of Besançon, 25000 Besançon, FranceChrono-Environnement UMR 6249 CNRS, Bourgogne Franche-Comté University, 25000 Besançon, FranceChrono-Environnement UMR 6249 CNRS, Bourgogne Franche-Comté University, 25000 Besançon, FranceParasitology-Mycology Department, University Hospital of Besançon, 25000 Besançon, FranceParasitology-Mycology Department, University Hospital of Besançon, 25000 Besançon, FranceParasitology-Mycology Department, University Hospital of Besançon, 25000 Besançon, France<b>Background:</b> Wood chipping has been described as a potential hotspot for the selection of azole-resistant <i>Aspergillus fumigatus</i> (AR<i>Af</i>). We previously reported AR<i>Af</i> isolates in sawmills (Eastern France), most of which contained the TR<sub>34</sub>/L98H mutation. <b>Methods:</b> To study genotypic relatedness, microsatellite genotyping (short tandem repeat for <i>A. fumigatus</i> (STR<i>Af</i>)) was performed on 41 azole-susceptible <i>A. fumigatus</i> (AS<i>Af</i>) and 23 AR<i>Af</i> isolated from 18 sawmills and two clinical <i>A. fumigatus</i> (sensitive and resistant) isolated from a sinus sample of a woodworker. <b>Results:</b> Fifty-four unique multilocus genotypes (MLGs) were described among the 66 isolates: 13/24 AR<i>Af</i> and 41/42 AS<i>Af.</i> Allelic diversity was higher for AS<i>Af</i> than for AR<i>Af</i>. Among the 24 AR<i>Af</i>, five isolates had their own MLGs. Thirteen AR<i>Af</i> (54%) belonged to the same group, composed of four close MLGs, defined using Bruvo’s distance. Thirty-two of the 42 AS<i>Af</i> (76%) had their own MLGs and could not be grouped with the Bruvo’s distance cutoff used (0.2). <b>Conclusion:</b> Thus, at a regional scale and in the particular environment of the wood industry, common but also different distinct genotypes, even in the same sawmill, were identified. This suggests that the hypothesis of AR<i>Af</i> clonal expansion from a common strain is probably insufficient to explain genotype emergence and distribution.https://www.mdpi.com/2309-608X/6/3/120<i>Aspergillus fumigatus</i>azole-resistantsawmillmicrosatellitemultilocus genotype |
| spellingShingle | Audrey Jeanvoine Chloé Godeau Audrey Laboissière Gabriel Reboux Laurence Millon Steffi Rocchi Molecular Epidemiology of Azole-Resistant <i>Aspergillus fumigatus</i> in Sawmills of Eastern France by Microsatellite Genotyping Journal of Fungi <i>Aspergillus fumigatus</i> azole-resistant sawmill microsatellite multilocus genotype |
| title | Molecular Epidemiology of Azole-Resistant <i>Aspergillus fumigatus</i> in Sawmills of Eastern France by Microsatellite Genotyping |
| title_full | Molecular Epidemiology of Azole-Resistant <i>Aspergillus fumigatus</i> in Sawmills of Eastern France by Microsatellite Genotyping |
| title_fullStr | Molecular Epidemiology of Azole-Resistant <i>Aspergillus fumigatus</i> in Sawmills of Eastern France by Microsatellite Genotyping |
| title_full_unstemmed | Molecular Epidemiology of Azole-Resistant <i>Aspergillus fumigatus</i> in Sawmills of Eastern France by Microsatellite Genotyping |
| title_short | Molecular Epidemiology of Azole-Resistant <i>Aspergillus fumigatus</i> in Sawmills of Eastern France by Microsatellite Genotyping |
| title_sort | molecular epidemiology of azole resistant i aspergillus fumigatus i in sawmills of eastern france by microsatellite genotyping |
| topic | <i>Aspergillus fumigatus</i> azole-resistant sawmill microsatellite multilocus genotype |
| url | https://www.mdpi.com/2309-608X/6/3/120 |
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