Comparison of Linear Poly Ethylene Imine (LPEI) and Poly L-Lysine (PLL) in Fabrication of CHOK1 Cell-Loaded Multilayer Alginate Microcapsules
Purpose: Poly l-lysine (PLL) has been introduced as a strengthening covering layer for alginate microcapsules which are the most convenient way for cell encapsulation. Some disadvantages of PLL such as high price and low biocompatibility have prompted scientists to find better alternatives. Linear p...
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| Format: | Article |
| Language: | English |
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Tabriz University of Medical Sciences
2020-02-01
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| Series: | Advanced Pharmaceutical Bulletin |
| Subjects: | |
| Online Access: | https://apb.tbzmed.ac.ir/PDF/apb-27815 |
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| author | Fariba Hajifathaliha Arash Mahboubi Elham Mohit Noushin Bolourchian Vahid Khalaj Leila Nematollahi |
| author_facet | Fariba Hajifathaliha Arash Mahboubi Elham Mohit Noushin Bolourchian Vahid Khalaj Leila Nematollahi |
| author_sort | Fariba Hajifathaliha |
| collection | DOAJ |
| description | Purpose: Poly l-lysine (PLL) has been introduced as a strengthening covering layer for alginate microcapsules which are the most convenient way for cell encapsulation. Some disadvantages of PLL such as high price and low biocompatibility have prompted scientists to find better alternatives. Linear poly ethylene imine (LPEI), thanks to its highly similar structure to PLL, could be considered as a proper cost-effective alternative. In this study LPEI and PLL were compared as covering layers of cell-loaded alginate-LPEI-alginate (cALA) and alginate-PLL-alginate (cAPA) microcapsules. Methods: In addition to the physico-mechanical properties, the encapsulation efficiency, cell survival post encapsulation, cell viability, and cellular metabolic activity within the microcapsules were evaluated using trypan blue, live/dead cell staining, and MTT test, respectively. Results: Physico-mechanical evaluation of the microcapsules revealed that the cell microencapsulation process did not affect their shape, size, and mechanical stability. Although the encapsulation efficiency for cALA and cAPA was not different (P>0.05), cell survival post encapsulation was higher in cALA than in cAPA (P<0.05) which could be the reason for the higher cell viability and also cellular metabolic activity within these microcapsules in comparison to cAPA. Conclusion: Here, based on these results, ALA could be introduced as a preferable alternative to APA for cell encapsulation. |
| first_indexed | 2024-04-14T04:06:34Z |
| format | Article |
| id | doaj.art-eac38c3b31344bcaaf59255b3056f8d2 |
| institution | Directory Open Access Journal |
| issn | 2228-5881 2251-7308 |
| language | English |
| last_indexed | 2024-04-14T04:06:34Z |
| publishDate | 2020-02-01 |
| publisher | Tabriz University of Medical Sciences |
| record_format | Article |
| series | Advanced Pharmaceutical Bulletin |
| spelling | doaj.art-eac38c3b31344bcaaf59255b3056f8d22022-12-22T02:13:20ZengTabriz University of Medical SciencesAdvanced Pharmaceutical Bulletin2228-58812251-73082020-02-0110229029610.34172/apb.2020.035apb-27815Comparison of Linear Poly Ethylene Imine (LPEI) and Poly L-Lysine (PLL) in Fabrication of CHOK1 Cell-Loaded Multilayer Alginate MicrocapsulesFariba Hajifathaliha0Arash Mahboubi1Elham Mohit2Noushin Bolourchian3Vahid Khalaj4Leila Nematollahi5Food Safety Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Food Safety Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Department of Pharmaceutical Biotechnology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Student Research Committee, Department of Pharmaceutics, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran.Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran.Purpose: Poly l-lysine (PLL) has been introduced as a strengthening covering layer for alginate microcapsules which are the most convenient way for cell encapsulation. Some disadvantages of PLL such as high price and low biocompatibility have prompted scientists to find better alternatives. Linear poly ethylene imine (LPEI), thanks to its highly similar structure to PLL, could be considered as a proper cost-effective alternative. In this study LPEI and PLL were compared as covering layers of cell-loaded alginate-LPEI-alginate (cALA) and alginate-PLL-alginate (cAPA) microcapsules. Methods: In addition to the physico-mechanical properties, the encapsulation efficiency, cell survival post encapsulation, cell viability, and cellular metabolic activity within the microcapsules were evaluated using trypan blue, live/dead cell staining, and MTT test, respectively. Results: Physico-mechanical evaluation of the microcapsules revealed that the cell microencapsulation process did not affect their shape, size, and mechanical stability. Although the encapsulation efficiency for cALA and cAPA was not different (P>0.05), cell survival post encapsulation was higher in cALA than in cAPA (P<0.05) which could be the reason for the higher cell viability and also cellular metabolic activity within these microcapsules in comparison to cAPA. Conclusion: Here, based on these results, ALA could be introduced as a preferable alternative to APA for cell encapsulation.https://apb.tbzmed.ac.ir/PDF/apb-27815alginic acidcell microencapsulationchok1poly ethylene iminepoly l-lysine |
| spellingShingle | Fariba Hajifathaliha Arash Mahboubi Elham Mohit Noushin Bolourchian Vahid Khalaj Leila Nematollahi Comparison of Linear Poly Ethylene Imine (LPEI) and Poly L-Lysine (PLL) in Fabrication of CHOK1 Cell-Loaded Multilayer Alginate Microcapsules Advanced Pharmaceutical Bulletin alginic acid cell microencapsulation chok1 poly ethylene imine poly l-lysine |
| title | Comparison of Linear Poly Ethylene Imine (LPEI) and Poly L-Lysine (PLL) in Fabrication of CHOK1 Cell-Loaded Multilayer Alginate Microcapsules |
| title_full | Comparison of Linear Poly Ethylene Imine (LPEI) and Poly L-Lysine (PLL) in Fabrication of CHOK1 Cell-Loaded Multilayer Alginate Microcapsules |
| title_fullStr | Comparison of Linear Poly Ethylene Imine (LPEI) and Poly L-Lysine (PLL) in Fabrication of CHOK1 Cell-Loaded Multilayer Alginate Microcapsules |
| title_full_unstemmed | Comparison of Linear Poly Ethylene Imine (LPEI) and Poly L-Lysine (PLL) in Fabrication of CHOK1 Cell-Loaded Multilayer Alginate Microcapsules |
| title_short | Comparison of Linear Poly Ethylene Imine (LPEI) and Poly L-Lysine (PLL) in Fabrication of CHOK1 Cell-Loaded Multilayer Alginate Microcapsules |
| title_sort | comparison of linear poly ethylene imine lpei and poly l lysine pll in fabrication of chok1 cell loaded multilayer alginate microcapsules |
| topic | alginic acid cell microencapsulation chok1 poly ethylene imine poly l-lysine |
| url | https://apb.tbzmed.ac.ir/PDF/apb-27815 |
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