| Summary: | <i>Odontotermes obesus</i> (Blattodea: Termitidae) is a prevalent subterranean wood-eating termite species that causes damage to mature trees, saplings and seedlings. The efficacy of most synthetic insecticides against this notorious pest has been compromised primarily because of its enigmatic feeding behavior and development of resistance to a number of insecticides. It has therefore become necessary to explore other alternative biologically sound and low-impact termite control methods, particularly for use in forests. Hence, this study was designed to verify the efficacy of different indigenous EPN isolates (<i>Steinernema carpocapsae</i>, <i>Heterorhabditis bacteriophora</i> and <i>Heterorhabditis indica</i>) against workers of <i>Odontotermes obesus</i>. The pathogenicity of each nematode isolate was assessed in laboratory conditions using filter paper and sawdust bioassay at two different temperatures (16 ± 1 and 26 ± 1 °C). Additionally, the efficacy of the nematode species was also assessed in field conditions. The results of the experiments revealed that the mortality of termite workers was more pronounced in sawdust bioassay in comparison with filter paper bioassay at both the tested temperatures. The mortality response in both bioassays was more pronounced at the higher temperature. A significantly higher mortality was recorded at both tested temperatures for <i>S. carpocapsae</i> followed by <i>H. bacteriophora</i> and <i>H. indica</i>. A dose-dependent positive mortality response was also recorded at both tested temperatures. Similar to the laboratory trials, the field applications of the three tested nematode species showed that maximum mortality was recorded for <i>S. carpocapsae</i> followed by <i>H. bacteriophora</i> and <i>H. indica.</i> It was therefore concluded that indigenous EPNs can provide more effective control of termites, possibly because of their direct interaction with pest species in the soil and the possibility of causing secondary infection through infected cadavers.
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