Synthesis and Characterization of Exopolysaccharide Encapsulated PCL/Gelatin Skin Substitute for Full-Thickness Wound Regeneration
Loss of skin integrity can lead to serious problems and even death. In this study, for the first time, the effect of exopolysaccharide (EPS) produced by cold-adapted yeast <i>R. mucilaginosa</i> sp. GUMS16 on a full-thickness wound in rats was evaluated. The GUMS16 strain’s EPS was preci...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-03-01
|
| Series: | Polymers |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4360/13/6/854 |
| _version_ | 1797541867654479872 |
|---|---|
| author | Ahmad Hivechi Peiman Brouki Milan Khashayar Modabberi Moein Amoupour Kaveh Ebrahimzadeh Amir Reza Gholipour Faezeh Sedighi Naser Amini S. Hajir Bahrami Alireza Rezapour Masoud Hamidi Cédric Delattre |
| author_facet | Ahmad Hivechi Peiman Brouki Milan Khashayar Modabberi Moein Amoupour Kaveh Ebrahimzadeh Amir Reza Gholipour Faezeh Sedighi Naser Amini S. Hajir Bahrami Alireza Rezapour Masoud Hamidi Cédric Delattre |
| author_sort | Ahmad Hivechi |
| collection | DOAJ |
| description | Loss of skin integrity can lead to serious problems and even death. In this study, for the first time, the effect of exopolysaccharide (EPS) produced by cold-adapted yeast <i>R. mucilaginosa</i> sp. GUMS16 on a full-thickness wound in rats was evaluated. The GUMS16 strain’s EPS was precipitated by adding cold ethanol and then lyophilized. Afterward, the EPS with polycaprolactone (PCL) and gelatin was fabricated into nanofibers with two single-needle and double-needle procedures. The rats’ full-thickness wounds were treated with nanofibers and Hematoxylin and eosin (H&E) and Masson’s Trichrome staining was done for studying the wound healing in rats. Obtained results from SEM, DLS, FTIR, and TGA showed that EPS has a carbohydrate chemical structure with an average diameter of 40 nm. Cell viability assessments showed that the 2% EPS loaded sample exhibits the highest cell activity. Moreover, in vivo implantation of nanofiber webs on the full-thickness wound on rat models displayed a faster healing rate when EPS was loaded into a nanofiber. These results suggest that the produced EPS can be used for skin tissue engineering applications. |
| first_indexed | 2024-03-10T13:21:46Z |
| format | Article |
| id | doaj.art-eac578db7a394e76ad8ada7f8dea2d97 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-10T13:21:46Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-eac578db7a394e76ad8ada7f8dea2d972023-11-21T09:58:39ZengMDPI AGPolymers2073-43602021-03-0113685410.3390/polym13060854Synthesis and Characterization of Exopolysaccharide Encapsulated PCL/Gelatin Skin Substitute for Full-Thickness Wound RegenerationAhmad Hivechi0Peiman Brouki Milan1Khashayar Modabberi2Moein Amoupour3Kaveh Ebrahimzadeh4Amir Reza Gholipour5Faezeh Sedighi6Naser Amini7S. Hajir Bahrami8Alireza Rezapour9Masoud Hamidi10Cédric Delattre11Department of Textile Engineering, School of Materials and Advanced Processing, Amirkabir University of Technology, Tehran 1591639675, IranCellular and Molecular Research Center, Iran University of Medical Sciences, Tehran 1591639675, IranDepartment of Medical Biotechnology, Faculty of Paramedicine, Guilan University of Medical Sciences, Rasht 4477166595, IranDepartment of Medical Biotechnology, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran 1591639675, IranDepartment of Neurosurgery, Loghman Hakim Hospital, Shahid Beheshti University of Medical Sciences, Tehran 1591639675, IranDepartment of Medical Biotechnology, Faculty of Paramedicine, Guilan University of Medical Sciences, Rasht 4477166595, IranDepartment of Medical Biotechnology, Faculty of Paramedicine, Guilan University of Medical Sciences, Rasht 4477166595, IranCellular and Molecular Research Center, Iran University of Medical Sciences, Tehran 1591639675, IranDepartment of Textile Engineering, School of Materials and Advanced Processing, Amirkabir University of Technology, Tehran 1591639675, IranDepartment of Tissue Engineering, School of Medicine, Qom University of Medical Sciences, Qom 3716993456, IranDepartment of Medical Biotechnology, Faculty of Paramedicine, Guilan University of Medical Sciences, Rasht 4477166595, IranUniversité Clermont Auvergne, CNRS, Clermont Auvergne INP, Institut Pascal, F-63000 Clermont-Ferrand, FranceLoss of skin integrity can lead to serious problems and even death. In this study, for the first time, the effect of exopolysaccharide (EPS) produced by cold-adapted yeast <i>R. mucilaginosa</i> sp. GUMS16 on a full-thickness wound in rats was evaluated. The GUMS16 strain’s EPS was precipitated by adding cold ethanol and then lyophilized. Afterward, the EPS with polycaprolactone (PCL) and gelatin was fabricated into nanofibers with two single-needle and double-needle procedures. The rats’ full-thickness wounds were treated with nanofibers and Hematoxylin and eosin (H&E) and Masson’s Trichrome staining was done for studying the wound healing in rats. Obtained results from SEM, DLS, FTIR, and TGA showed that EPS has a carbohydrate chemical structure with an average diameter of 40 nm. Cell viability assessments showed that the 2% EPS loaded sample exhibits the highest cell activity. Moreover, in vivo implantation of nanofiber webs on the full-thickness wound on rat models displayed a faster healing rate when EPS was loaded into a nanofiber. These results suggest that the produced EPS can be used for skin tissue engineering applications.https://www.mdpi.com/2073-4360/13/6/854nanofiberwound dressingexopolysaccharidetissue regeneration |
| spellingShingle | Ahmad Hivechi Peiman Brouki Milan Khashayar Modabberi Moein Amoupour Kaveh Ebrahimzadeh Amir Reza Gholipour Faezeh Sedighi Naser Amini S. Hajir Bahrami Alireza Rezapour Masoud Hamidi Cédric Delattre Synthesis and Characterization of Exopolysaccharide Encapsulated PCL/Gelatin Skin Substitute for Full-Thickness Wound Regeneration Polymers nanofiber wound dressing exopolysaccharide tissue regeneration |
| title | Synthesis and Characterization of Exopolysaccharide Encapsulated PCL/Gelatin Skin Substitute for Full-Thickness Wound Regeneration |
| title_full | Synthesis and Characterization of Exopolysaccharide Encapsulated PCL/Gelatin Skin Substitute for Full-Thickness Wound Regeneration |
| title_fullStr | Synthesis and Characterization of Exopolysaccharide Encapsulated PCL/Gelatin Skin Substitute for Full-Thickness Wound Regeneration |
| title_full_unstemmed | Synthesis and Characterization of Exopolysaccharide Encapsulated PCL/Gelatin Skin Substitute for Full-Thickness Wound Regeneration |
| title_short | Synthesis and Characterization of Exopolysaccharide Encapsulated PCL/Gelatin Skin Substitute for Full-Thickness Wound Regeneration |
| title_sort | synthesis and characterization of exopolysaccharide encapsulated pcl gelatin skin substitute for full thickness wound regeneration |
| topic | nanofiber wound dressing exopolysaccharide tissue regeneration |
| url | https://www.mdpi.com/2073-4360/13/6/854 |
| work_keys_str_mv | AT ahmadhivechi synthesisandcharacterizationofexopolysaccharideencapsulatedpclgelatinskinsubstituteforfullthicknesswoundregeneration AT peimanbroukimilan synthesisandcharacterizationofexopolysaccharideencapsulatedpclgelatinskinsubstituteforfullthicknesswoundregeneration AT khashayarmodabberi synthesisandcharacterizationofexopolysaccharideencapsulatedpclgelatinskinsubstituteforfullthicknesswoundregeneration AT moeinamoupour synthesisandcharacterizationofexopolysaccharideencapsulatedpclgelatinskinsubstituteforfullthicknesswoundregeneration AT kavehebrahimzadeh synthesisandcharacterizationofexopolysaccharideencapsulatedpclgelatinskinsubstituteforfullthicknesswoundregeneration AT amirrezagholipour synthesisandcharacterizationofexopolysaccharideencapsulatedpclgelatinskinsubstituteforfullthicknesswoundregeneration AT faezehsedighi synthesisandcharacterizationofexopolysaccharideencapsulatedpclgelatinskinsubstituteforfullthicknesswoundregeneration AT naseramini synthesisandcharacterizationofexopolysaccharideencapsulatedpclgelatinskinsubstituteforfullthicknesswoundregeneration AT shajirbahrami synthesisandcharacterizationofexopolysaccharideencapsulatedpclgelatinskinsubstituteforfullthicknesswoundregeneration AT alirezarezapour synthesisandcharacterizationofexopolysaccharideencapsulatedpclgelatinskinsubstituteforfullthicknesswoundregeneration AT masoudhamidi synthesisandcharacterizationofexopolysaccharideencapsulatedpclgelatinskinsubstituteforfullthicknesswoundregeneration AT cedricdelattre synthesisandcharacterizationofexopolysaccharideencapsulatedpclgelatinskinsubstituteforfullthicknesswoundregeneration |