Isolation and Characterisation of Anti-diabetic Pharmacological Activities of Phytoestrogens
Background: Isoflavones class of phytoestrogens including, genestein, daidzein and formononetin found in human dietary and show wide range of biological effects. These plant derived compounds have been shown to play a beneficial role in obesity and diabetes. Objective: In this study the impact of th...
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| Format: | Article |
| Language: | English |
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Institue of Medicinal Plants, ACECR
2016-08-01
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| Series: | Journal of Medicinal Plants |
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| Online Access: | http://jmp.ir/article-1-1474-en.html |
| _version_ | 1818716238316044288 |
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| author | D Roostaei Sh Rezazadeh S Sharafi K White |
| author_facet | D Roostaei Sh Rezazadeh S Sharafi K White |
| author_sort | D Roostaei |
| collection | DOAJ |
| description | Background: Isoflavones class of phytoestrogens including, genestein, daidzein and formononetin found in human dietary and show wide range of biological effects. These plant derived compounds have been shown to play a beneficial role in obesity and diabetes.
Objective: In this study the impact of these phytoestrogens on glucose uptake in HepG2 cell were compared.
Methods: Glucose uptake measurement was performed using 2-(N-(7-nitrobenzin-2-oxa-1, 3- diazol-4-yl) amino)-2-deoxyglucose (2-NBDG) and Omega FluoStar plate reader. Incubation of cells (104/ml, in 24 well plate at 37 °C in 5% CO2 / air) with three phytoestrogens at concentration of 10-4 M to 10-9M in two studies mood, short term treatment (one hour) and long term treatment (24 hours) was tested.
Results: The data revealed, daidzein stimulates uptake of glucose, with a greater effect after a short treatment of one hour compared with treatment 24 hours. genistein exerted slightly inhibitory effect after one hour treatment compared with control, with the exception of treatment at 1 µM, which stimulated uptake about three-fold compared with control. Longer treatments with 10-4M to 10-6 M genistein resulted in gradual increase in glucose uptake to 2.4 times more than control, and thereafter a decline. A short treatment with formononetin inhibited glucose uptake, while longer treatments had variable effects, with an approximately two fold stimulation across a range of concentrations
Conclusion: Overall HepG2 cells showed a significant increase in glucose uptake after treatment with phytoestrogens compared to the control. There was significant difference in glucose uptake between short and long term treatments, as indicated by two-way ANOVA. |
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| issn | 2717-204X 2717-2058 |
| language | English |
| last_indexed | 2024-12-17T19:16:05Z |
| publishDate | 2016-08-01 |
| publisher | Institue of Medicinal Plants, ACECR |
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| series | Journal of Medicinal Plants |
| spelling | doaj.art-eacd1ed6324a4eb8a5878e28126235632022-12-21T21:35:46ZengInstitue of Medicinal Plants, ACECRJournal of Medicinal Plants2717-204X2717-20582016-08-011559145152Isolation and Characterisation of Anti-diabetic Pharmacological Activities of PhytoestrogensD Roostaei0Sh Rezazadeh1S Sharafi2K White3 Faculty of Life Sciences and Computing, London Metropolitan University, London, UK Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran The Royal College of General Practitioners (RCGP), London, UK Faculty of Life Sciences and Computing, London Metropolitan University, London, UK Background: Isoflavones class of phytoestrogens including, genestein, daidzein and formononetin found in human dietary and show wide range of biological effects. These plant derived compounds have been shown to play a beneficial role in obesity and diabetes. Objective: In this study the impact of these phytoestrogens on glucose uptake in HepG2 cell were compared. Methods: Glucose uptake measurement was performed using 2-(N-(7-nitrobenzin-2-oxa-1, 3- diazol-4-yl) amino)-2-deoxyglucose (2-NBDG) and Omega FluoStar plate reader. Incubation of cells (104/ml, in 24 well plate at 37 °C in 5% CO2 / air) with three phytoestrogens at concentration of 10-4 M to 10-9M in two studies mood, short term treatment (one hour) and long term treatment (24 hours) was tested. Results: The data revealed, daidzein stimulates uptake of glucose, with a greater effect after a short treatment of one hour compared with treatment 24 hours. genistein exerted slightly inhibitory effect after one hour treatment compared with control, with the exception of treatment at 1 µM, which stimulated uptake about three-fold compared with control. Longer treatments with 10-4M to 10-6 M genistein resulted in gradual increase in glucose uptake to 2.4 times more than control, and thereafter a decline. A short treatment with formononetin inhibited glucose uptake, while longer treatments had variable effects, with an approximately two fold stimulation across a range of concentrations Conclusion: Overall HepG2 cells showed a significant increase in glucose uptake after treatment with phytoestrogens compared to the control. There was significant difference in glucose uptake between short and long term treatments, as indicated by two-way ANOVA.http://jmp.ir/article-1-1474-en.htmlphytoestrogenshyperglycemia<i> in vitro</i> study2-nbdg |
| spellingShingle | D Roostaei Sh Rezazadeh S Sharafi K White Isolation and Characterisation of Anti-diabetic Pharmacological Activities of Phytoestrogens Journal of Medicinal Plants phytoestrogens hyperglycemia <i> in vitro</i> study 2-nbdg |
| title | Isolation and Characterisation of Anti-diabetic Pharmacological Activities of Phytoestrogens |
| title_full | Isolation and Characterisation of Anti-diabetic Pharmacological Activities of Phytoestrogens |
| title_fullStr | Isolation and Characterisation of Anti-diabetic Pharmacological Activities of Phytoestrogens |
| title_full_unstemmed | Isolation and Characterisation of Anti-diabetic Pharmacological Activities of Phytoestrogens |
| title_short | Isolation and Characterisation of Anti-diabetic Pharmacological Activities of Phytoestrogens |
| title_sort | isolation and characterisation of anti diabetic pharmacological activities of phytoestrogens |
| topic | phytoestrogens hyperglycemia <i> in vitro</i> study 2-nbdg |
| url | http://jmp.ir/article-1-1474-en.html |
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