The Study of HLA-G Gene and Protein Expression in Patients withRecurrent Miscarriage
Purpose: Although it has been frequently confirmed that HLA-G plays an important role in thereproduction and pregnancy, the pattern of HLA-G gene and its protein expression are rarelyaddressed in studies. Therefore we conducted this study in regard to evaluate the HLA-G geneand its protein expressio...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Tabriz University of Medical Sciences
2019-02-01
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| Series: | Advanced Pharmaceutical Bulletin |
| Subjects: | |
| Online Access: | https://apb.tbzmed.ac.ir/PDF/apb-19559 |
| _version_ | 1811318888200142848 |
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| author | Elnaz Mosaferi Nazila Alizadeh Gharamaleki Laya Farzadi Jafar Majidi Zohreh Babaloo Tohid kazemi Mehrnoosh Ramezani Meraj Tabatabaei Hamid Ahmadi Leili Aghebati maleki Behzad Baradaran |
| author_facet | Elnaz Mosaferi Nazila Alizadeh Gharamaleki Laya Farzadi Jafar Majidi Zohreh Babaloo Tohid kazemi Mehrnoosh Ramezani Meraj Tabatabaei Hamid Ahmadi Leili Aghebati maleki Behzad Baradaran |
| author_sort | Elnaz Mosaferi |
| collection | DOAJ |
| description | Purpose: Although it has been frequently confirmed that HLA-G plays an important role in thereproduction and pregnancy, the pattern of HLA-G gene and its protein expression are rarelyaddressed in studies. Therefore we conducted this study in regard to evaluate the HLA-G geneand its protein expression in the women’s placenta with recurrent miscarriage.Methods: Placental samples were obtained from the women who were admitted for deliveryor abortion in Al Zahra and Taleghani hospitals, Tabriz, Iran. HLA-G gene expression wasdetermined by real-time polymerase chain reaction (PCR) and HLA-G protein expression wasassessed by western blotting and immunofluorescence staining in the tissue samples.Results: The results showed a significant decrease in the expression of gene and proteins ofHLA-G in the women with recurrent miscarriage compared to the control placental tissues.Conclusion: According to the obtained results, it was concluded that the decrement of HLA-Ggene and protein expressions are associated with recurrent miscarriage. Since there areconflicting results from other studies, it is suggested to conduct a more comprehensive similarstudy with greater sample size. |
| first_indexed | 2024-04-13T12:33:05Z |
| format | Article |
| id | doaj.art-eacef3fbda694eb98fdd010ca7450a11 |
| institution | Directory Open Access Journal |
| issn | 2228-5881 2251-7308 |
| language | English |
| last_indexed | 2024-04-13T12:33:05Z |
| publishDate | 2019-02-01 |
| publisher | Tabriz University of Medical Sciences |
| record_format | Article |
| series | Advanced Pharmaceutical Bulletin |
| spelling | doaj.art-eacef3fbda694eb98fdd010ca7450a112022-12-22T02:46:47ZengTabriz University of Medical SciencesAdvanced Pharmaceutical Bulletin2228-58812251-73082019-02-0191707510.15171/apb.2019.009apb-19559The Study of HLA-G Gene and Protein Expression in Patients withRecurrent MiscarriageElnaz Mosaferi0Nazila Alizadeh Gharamaleki1Laya Farzadi2Jafar Majidi3Zohreh Babaloo4Tohid kazemi5Mehrnoosh Ramezani6Meraj Tabatabaei7Hamid Ahmadi8Leili Aghebati maleki9Behzad Baradaran10Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.Women Reproductive Health Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.Department of Biochemistry, school of Medicine, Ardabil University of Medical Science, Ardabil Iran.Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.Purpose: Although it has been frequently confirmed that HLA-G plays an important role in thereproduction and pregnancy, the pattern of HLA-G gene and its protein expression are rarelyaddressed in studies. Therefore we conducted this study in regard to evaluate the HLA-G geneand its protein expression in the women’s placenta with recurrent miscarriage.Methods: Placental samples were obtained from the women who were admitted for deliveryor abortion in Al Zahra and Taleghani hospitals, Tabriz, Iran. HLA-G gene expression wasdetermined by real-time polymerase chain reaction (PCR) and HLA-G protein expression wasassessed by western blotting and immunofluorescence staining in the tissue samples.Results: The results showed a significant decrease in the expression of gene and proteins ofHLA-G in the women with recurrent miscarriage compared to the control placental tissues.Conclusion: According to the obtained results, it was concluded that the decrement of HLA-Ggene and protein expressions are associated with recurrent miscarriage. Since there areconflicting results from other studies, it is suggested to conduct a more comprehensive similarstudy with greater sample size.https://apb.tbzmed.ac.ir/PDF/apb-19559HLA-GPlacentaReal-Time PCRMiscarriage |
| spellingShingle | Elnaz Mosaferi Nazila Alizadeh Gharamaleki Laya Farzadi Jafar Majidi Zohreh Babaloo Tohid kazemi Mehrnoosh Ramezani Meraj Tabatabaei Hamid Ahmadi Leili Aghebati maleki Behzad Baradaran The Study of HLA-G Gene and Protein Expression in Patients withRecurrent Miscarriage Advanced Pharmaceutical Bulletin HLA-G Placenta Real-Time PCR Miscarriage |
| title | The Study of HLA-G Gene and Protein Expression in Patients withRecurrent Miscarriage |
| title_full | The Study of HLA-G Gene and Protein Expression in Patients withRecurrent Miscarriage |
| title_fullStr | The Study of HLA-G Gene and Protein Expression in Patients withRecurrent Miscarriage |
| title_full_unstemmed | The Study of HLA-G Gene and Protein Expression in Patients withRecurrent Miscarriage |
| title_short | The Study of HLA-G Gene and Protein Expression in Patients withRecurrent Miscarriage |
| title_sort | study of hla g gene and protein expression in patients withrecurrent miscarriage |
| topic | HLA-G Placenta Real-Time PCR Miscarriage |
| url | https://apb.tbzmed.ac.ir/PDF/apb-19559 |
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