Performance of Xpert MTB/RIF in comparison with light-emitting diode-fluorescence microscopy and culture for detecting tuberculosis in pulmonary and extrapulmonary specimens in Bamako, Mali
Background: The diagnosis of tuberculosis (TB) has mostly been relied on a long-used method called sputum smear microscopy. In 2010, Xpert MTB/RIF assay was approved by the World Health Organization for simultaneous TB diagnosis and detection of resistance. Our current study was undertaken to compar...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2020-01-01
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| Series: | International Journal of Mycobacteriology |
| Subjects: | |
| Online Access: | http://www.ijmyco.org/article.asp?issn=2212-5531;year=2020;volume=9;issue=4;spage=397;epage=404;aulast=Combo |
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| author | Togo Antieme Combo Georges Traore Aissata Diallo Fatimata Coulibaly Cheick Abou Coulibaly Gagni Somboro Anou Moise Degoga Boureima Somboro Amadou Drame Hawa Baye Dembele Bindongo Price Polycarpe Kone Bourahima Sanogo Moumine Sarro Yeya Dit Sadio Kodio Ousmane Baya Bocar Tolofoudie Mohamed Traore Aissata Boubakar Bane Sidy Maiga Mamoudou Belson Michael Doumbia Seydou Diarra Bassirou I Maiga Ibrahima Diallo Souleymane |
| author_facet | Togo Antieme Combo Georges Traore Aissata Diallo Fatimata Coulibaly Cheick Abou Coulibaly Gagni Somboro Anou Moise Degoga Boureima Somboro Amadou Drame Hawa Baye Dembele Bindongo Price Polycarpe Kone Bourahima Sanogo Moumine Sarro Yeya Dit Sadio Kodio Ousmane Baya Bocar Tolofoudie Mohamed Traore Aissata Boubakar Bane Sidy Maiga Mamoudou Belson Michael Doumbia Seydou Diarra Bassirou I Maiga Ibrahima Diallo Souleymane |
| author_sort | Togo Antieme Combo Georges |
| collection | DOAJ |
| description | Background: The diagnosis of tuberculosis (TB) has mostly been relied on a long-used method called sputum smear microscopy. In 2010, Xpert MTB/RIF assay was approved by the World Health Organization for simultaneous TB diagnosis and detection of resistance. Our current study was undertaken to compare the diagnostic performance of Xpert MTB/RIF assay to auramine staining-based light-emitting diode-Fluorescence Microscopy (LED-FM) considering culture as the gold standard method for pulmonary and extrapulmonary TB. Method: Pulmonary and extrapulmonary specimens of suspected TB patients were examined in this study. From January 2016 to June 2019, sputum, urine, superficial swabs, gastric aspirates, and pleural infusion specimens were collected from new and previously treated TB individuals. Specimens were examined using Xpert MTB/RIF, LED-FM, and Mycobacterium culture techniques to evaluate their performance. Results: A total of 697 suspected TB samples were included in this analysis, and of these, 469 (67.29%) were positive for all three used methods. The overall sensitivities, specificities, and positive and negative predictive values were 99.6%, 62.0%, 88.4%, and 98.2% for Xpert MTB/RIF and 88.0%, 95.6%, 99.0%, and 60.7% for LED-FM, respectively, compared to culture method. Conclusion: The sensitivity of Xpert MTB/RIF assay was observed to be higher than the LED-FM method, thus suggesting this molecular technique as a promising tool for the diagnosis of pulmonary and extrapulmonary TB, which will help in the management of TB infections in developing countries such as Mali. |
| first_indexed | 2024-12-20T09:46:52Z |
| format | Article |
| id | doaj.art-ead40249cd3d417ca329f7b01e83d86a |
| institution | Directory Open Access Journal |
| issn | 2212-5531 2212-554X |
| language | English |
| last_indexed | 2024-12-20T09:46:52Z |
| publishDate | 2020-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | International Journal of Mycobacteriology |
| spelling | doaj.art-ead40249cd3d417ca329f7b01e83d86a2022-12-21T19:44:43ZengWolters Kluwer Medknow PublicationsInternational Journal of Mycobacteriology2212-55312212-554X2020-01-019439740410.4103/ijmy.ijmy_171_20Performance of Xpert MTB/RIF in comparison with light-emitting diode-fluorescence microscopy and culture for detecting tuberculosis in pulmonary and extrapulmonary specimens in Bamako, MaliTogo Antieme Combo GeorgesTraore AissataDiallo FatimataCoulibaly Cheick AbouCoulibaly GagniSomboro Anou MoiseDegoga BoureimaSomboro AmadouDrame Hawa BayeDembele Bindongo Price PolycarpeKone BourahimaSanogo MoumineSarro Yeya Dit SadioKodio OusmaneBaya BocarTolofoudie MohamedTraore Aissata BoubakarBane SidyMaiga MamoudouBelson MichaelDoumbia SeydouDiarra BassirouI Maiga IbrahimaDiallo SouleymaneBackground: The diagnosis of tuberculosis (TB) has mostly been relied on a long-used method called sputum smear microscopy. In 2010, Xpert MTB/RIF assay was approved by the World Health Organization for simultaneous TB diagnosis and detection of resistance. Our current study was undertaken to compare the diagnostic performance of Xpert MTB/RIF assay to auramine staining-based light-emitting diode-Fluorescence Microscopy (LED-FM) considering culture as the gold standard method for pulmonary and extrapulmonary TB. Method: Pulmonary and extrapulmonary specimens of suspected TB patients were examined in this study. From January 2016 to June 2019, sputum, urine, superficial swabs, gastric aspirates, and pleural infusion specimens were collected from new and previously treated TB individuals. Specimens were examined using Xpert MTB/RIF, LED-FM, and Mycobacterium culture techniques to evaluate their performance. Results: A total of 697 suspected TB samples were included in this analysis, and of these, 469 (67.29%) were positive for all three used methods. The overall sensitivities, specificities, and positive and negative predictive values were 99.6%, 62.0%, 88.4%, and 98.2% for Xpert MTB/RIF and 88.0%, 95.6%, 99.0%, and 60.7% for LED-FM, respectively, compared to culture method. Conclusion: The sensitivity of Xpert MTB/RIF assay was observed to be higher than the LED-FM method, thus suggesting this molecular technique as a promising tool for the diagnosis of pulmonary and extrapulmonary TB, which will help in the management of TB infections in developing countries such as Mali.http://www.ijmyco.org/article.asp?issn=2212-5531;year=2020;volume=9;issue=4;spage=397;epage=404;aulast=Combobamakoculturelight-emitting diode fluorescent microscopymalixpert mtb/rif |
| spellingShingle | Togo Antieme Combo Georges Traore Aissata Diallo Fatimata Coulibaly Cheick Abou Coulibaly Gagni Somboro Anou Moise Degoga Boureima Somboro Amadou Drame Hawa Baye Dembele Bindongo Price Polycarpe Kone Bourahima Sanogo Moumine Sarro Yeya Dit Sadio Kodio Ousmane Baya Bocar Tolofoudie Mohamed Traore Aissata Boubakar Bane Sidy Maiga Mamoudou Belson Michael Doumbia Seydou Diarra Bassirou I Maiga Ibrahima Diallo Souleymane Performance of Xpert MTB/RIF in comparison with light-emitting diode-fluorescence microscopy and culture for detecting tuberculosis in pulmonary and extrapulmonary specimens in Bamako, Mali International Journal of Mycobacteriology bamako culture light-emitting diode fluorescent microscopy mali xpert mtb/rif |
| title | Performance of Xpert MTB/RIF in comparison with light-emitting diode-fluorescence microscopy and culture for detecting tuberculosis in pulmonary and extrapulmonary specimens in Bamako, Mali |
| title_full | Performance of Xpert MTB/RIF in comparison with light-emitting diode-fluorescence microscopy and culture for detecting tuberculosis in pulmonary and extrapulmonary specimens in Bamako, Mali |
| title_fullStr | Performance of Xpert MTB/RIF in comparison with light-emitting diode-fluorescence microscopy and culture for detecting tuberculosis in pulmonary and extrapulmonary specimens in Bamako, Mali |
| title_full_unstemmed | Performance of Xpert MTB/RIF in comparison with light-emitting diode-fluorescence microscopy and culture for detecting tuberculosis in pulmonary and extrapulmonary specimens in Bamako, Mali |
| title_short | Performance of Xpert MTB/RIF in comparison with light-emitting diode-fluorescence microscopy and culture for detecting tuberculosis in pulmonary and extrapulmonary specimens in Bamako, Mali |
| title_sort | performance of xpert mtb rif in comparison with light emitting diode fluorescence microscopy and culture for detecting tuberculosis in pulmonary and extrapulmonary specimens in bamako mali |
| topic | bamako culture light-emitting diode fluorescent microscopy mali xpert mtb/rif |
| url | http://www.ijmyco.org/article.asp?issn=2212-5531;year=2020;volume=9;issue=4;spage=397;epage=404;aulast=Combo |
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