Albumin nanoparticles Preparation, Characterization and In-Vitro Safety Evaluation
The goal of this study was to prepare and characterize albumin nanoparticles to be later used as a drug delivery system. The utilization of nanoparticles as a delivery system for antimicrobial drugs has arisen recently which solve many problems and enhance the traditional treatment with this antimic...
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| Format: | Article |
| Language: | English |
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Ain Shams University, Faculty of Women for Arts, Science & Education
2021-12-01
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| Series: | Journal of Scientific Research in Science |
| Subjects: | |
| Online Access: | https://jsrs.journals.ekb.eg/article_210692.html |
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| author | Dina G.S. Youssef Fatma A. Sallam Taher A. Salaheldin Samah F. Darwish Abeer E. El-Metwally Khaled Y. Farroh Adel S. Amin |
| author_facet | Dina G.S. Youssef Fatma A. Sallam Taher A. Salaheldin Samah F. Darwish Abeer E. El-Metwally Khaled Y. Farroh Adel S. Amin |
| author_sort | Dina G.S. Youssef |
| collection | DOAJ |
| description | The goal of this study was to prepare and characterize albumin nanoparticles to be later used as a drug delivery system. The utilization of nanoparticles as a delivery system for antimicrobial drugs has arisen recently which solve many problems and enhance the traditional treatment with this antimicrobial drugs. In this study, nanoparticles of bovine serum albumen were successfully obtained using a coacervation process (separation of proteins in two liquid phases in colloidal systems). The prepared nanoparticles were nearly spherical in shape and have smooth surface as determined by Transmission Electron Microscopy (TEM). The sizes of the obtained nanoparticles were 70 ± 10 nm with negative surface zeta potential. Additionally, the in vitro safety of albumin nanoparticles has been demonstrated. Both cytotoxicity and genotoxicity studies indicated that, there is no observed toxic effect of nano-albumin on lymphocyte cell line. Also, the results showed that the albumin nanoparticles enhances and promotes the response of immune system. |
| first_indexed | 2024-03-12T13:17:58Z |
| format | Article |
| id | doaj.art-ead59ef290074383a053d437bb746195 |
| institution | Directory Open Access Journal |
| issn | 2356-8364 2356-8372 |
| language | English |
| last_indexed | 2024-03-12T13:17:58Z |
| publishDate | 2021-12-01 |
| publisher | Ain Shams University, Faculty of Women for Arts, Science & Education |
| record_format | Article |
| series | Journal of Scientific Research in Science |
| spelling | doaj.art-ead59ef290074383a053d437bb7461952023-08-26T16:01:18ZengAin Shams University, Faculty of Women for Arts, Science & EducationJournal of Scientific Research in Science2356-83642356-83722021-12-01382183196doi:10.21608/JSRS.2021.210692Albumin nanoparticles Preparation, Characterization and In-Vitro Safety EvaluationDina G.S. Youssef0Fatma A. Sallam1Taher A. Salaheldin2Samah F. Darwish3Abeer E. El-Metwally4Khaled Y. Farroh5Adel S. Amin6Biotechnology Research Unit, Animal Reproduction Research Institute, Egypt.Faculty of Women for Arts, Science and Education, Ain Shams University, EgyptNanotechnology and Advanced Material Central Lab, Agriculture Research Center, Egypt; Mostafa Elsayed Nanotechnology Research Centre, British University in Egypt, Egypt.Biotechnology Research Unit, Animal Reproduction Research Institute, Egypt.Immunology Unit, Animal Reproduction Research Institute, Egypt.Nanotechnology Department, Agricultural Research Center, Giza, Egypt.Biotechnology Research Unit, Animal Reproduction Research Institute, Egypt.The goal of this study was to prepare and characterize albumin nanoparticles to be later used as a drug delivery system. The utilization of nanoparticles as a delivery system for antimicrobial drugs has arisen recently which solve many problems and enhance the traditional treatment with this antimicrobial drugs. In this study, nanoparticles of bovine serum albumen were successfully obtained using a coacervation process (separation of proteins in two liquid phases in colloidal systems). The prepared nanoparticles were nearly spherical in shape and have smooth surface as determined by Transmission Electron Microscopy (TEM). The sizes of the obtained nanoparticles were 70 ± 10 nm with negative surface zeta potential. Additionally, the in vitro safety of albumin nanoparticles has been demonstrated. Both cytotoxicity and genotoxicity studies indicated that, there is no observed toxic effect of nano-albumin on lymphocyte cell line. Also, the results showed that the albumin nanoparticles enhances and promotes the response of immune system. https://jsrs.journals.ekb.eg/article_210692.htmlalbumin nanoparticlescytotoxicgenotoxicproliferation activity |
| spellingShingle | Dina G.S. Youssef Fatma A. Sallam Taher A. Salaheldin Samah F. Darwish Abeer E. El-Metwally Khaled Y. Farroh Adel S. Amin Albumin nanoparticles Preparation, Characterization and In-Vitro Safety Evaluation Journal of Scientific Research in Science albumin nanoparticles cytotoxic genotoxic proliferation activity |
| title | Albumin nanoparticles Preparation, Characterization and In-Vitro Safety Evaluation |
| title_full | Albumin nanoparticles Preparation, Characterization and In-Vitro Safety Evaluation |
| title_fullStr | Albumin nanoparticles Preparation, Characterization and In-Vitro Safety Evaluation |
| title_full_unstemmed | Albumin nanoparticles Preparation, Characterization and In-Vitro Safety Evaluation |
| title_short | Albumin nanoparticles Preparation, Characterization and In-Vitro Safety Evaluation |
| title_sort | albumin nanoparticles preparation characterization and in vitro safety evaluation |
| topic | albumin nanoparticles cytotoxic genotoxic proliferation activity |
| url | https://jsrs.journals.ekb.eg/article_210692.html |
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