Zinc Oxide Nanoparticles Induces Apoptosis in Human Breast Cancer Cells via Caspase-8 and P53 Pathway
Nanoparticles are a special institution of substances with precise capabilities and significant applications in many biomedical fields. In the present work zinc oxide nanoparticles were prepared through sol-gel approach. The synthesised nanoparticles were identified through the usage of X-ray powder...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2019-03-01
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| Series: | Nano Biomedicine and Engineering |
| Subjects: | |
| Online Access: | https://www.sciopen.com/article/10.5101/nbe.v11i1.p35-43 |
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| author | Kadhem Haitham Ali Sumayah Abdulhussien Ibraheem Majid Sakhi Jabir Kadhim Afraa Ali Zainab Jihad Taqi Florin Mihailescu Dan |
| author_facet | Kadhem Haitham Ali Sumayah Abdulhussien Ibraheem Majid Sakhi Jabir Kadhim Afraa Ali Zainab Jihad Taqi Florin Mihailescu Dan |
| author_sort | Kadhem Haitham Ali |
| collection | DOAJ |
| description | Nanoparticles are a special institution of substances with precise capabilities and significant applications in many biomedical fields. In the present work zinc oxide nanoparticles were prepared through sol-gel approach. The synthesised nanoparticles were identified through the usage of X-ray powder diffraction (XRD), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). In-vitro anticancer activity of zinc oxide nanoparticles towards MCF-7 cell lines using numerous parameters was investigated. Zinc oxide nanoparticles were determined to exert cell growth arrest against MCF-7 cell lines. The anti-proliferative efficiency of ZnO nanoparticles was due to cell dying and inducing apoptosis that were confirmed by the usage of acridine orange/ethidium bromide dual staining, DAPI staining and genotoxicity assay. Reverse transcription polymerase chain reaction (RT- PCR) analysis achieved to identify the gene expression of Caspase-8, Caspase-9, and P53. The results suggested that ZnO nanoparticles might find a wide use in clinical applications and provide new drug recompense for chemotherapy drugs. |
| first_indexed | 2024-03-11T21:44:08Z |
| format | Article |
| id | doaj.art-ead6d92d13fb46329598b2de12bf09c2 |
| institution | Directory Open Access Journal |
| issn | 2150-5578 |
| language | English |
| last_indexed | 2025-03-20T02:13:40Z |
| publishDate | 2019-03-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Nano Biomedicine and Engineering |
| spelling | doaj.art-ead6d92d13fb46329598b2de12bf09c22024-10-03T08:08:48ZengTsinghua University PressNano Biomedicine and Engineering2150-55782019-03-01111354310.5101/nbe.v11i1.p35-43Zinc Oxide Nanoparticles Induces Apoptosis in Human Breast Cancer Cells via Caspase-8 and P53 PathwayKadhem Haitham Ali0Sumayah Abdulhussien Ibraheem1Majid Sakhi Jabir2Kadhim Afraa Ali3Zainab Jihad Taqi4Florin Mihailescu Dan5Department of Animal Physiology and Biophysics, Faculty of Biology, Bucharest, RomaniaDepartment of Animal Physiology and Biophysics, Faculty of Biology, Bucharest, RomaniaDepartment of Applied Science, University of Technology, Baghdad, IraqDepartment of Biology, College of Science, University of Al-Mustansiriyah, Baghdad, IraqDepartment of Applied Science, University of Technology, Baghdad, IraqDepartment of Animal Physiology and Biophysics, Faculty of Biology, Bucharest, RomaniaNanoparticles are a special institution of substances with precise capabilities and significant applications in many biomedical fields. In the present work zinc oxide nanoparticles were prepared through sol-gel approach. The synthesised nanoparticles were identified through the usage of X-ray powder diffraction (XRD), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). In-vitro anticancer activity of zinc oxide nanoparticles towards MCF-7 cell lines using numerous parameters was investigated. Zinc oxide nanoparticles were determined to exert cell growth arrest against MCF-7 cell lines. The anti-proliferative efficiency of ZnO nanoparticles was due to cell dying and inducing apoptosis that were confirmed by the usage of acridine orange/ethidium bromide dual staining, DAPI staining and genotoxicity assay. Reverse transcription polymerase chain reaction (RT- PCR) analysis achieved to identify the gene expression of Caspase-8, Caspase-9, and P53. The results suggested that ZnO nanoparticles might find a wide use in clinical applications and provide new drug recompense for chemotherapy drugs.https://www.sciopen.com/article/10.5101/nbe.v11i1.p35-43zinc oxide nanoparticlescytotoxicitymcf-7genotoxicitycaspase-8p53 |
| spellingShingle | Kadhem Haitham Ali Sumayah Abdulhussien Ibraheem Majid Sakhi Jabir Kadhim Afraa Ali Zainab Jihad Taqi Florin Mihailescu Dan Zinc Oxide Nanoparticles Induces Apoptosis in Human Breast Cancer Cells via Caspase-8 and P53 Pathway Nano Biomedicine and Engineering zinc oxide nanoparticles cytotoxicity mcf-7 genotoxicity caspase-8 p53 |
| title | Zinc Oxide Nanoparticles Induces Apoptosis in Human Breast Cancer Cells via Caspase-8 and P53 Pathway |
| title_full | Zinc Oxide Nanoparticles Induces Apoptosis in Human Breast Cancer Cells via Caspase-8 and P53 Pathway |
| title_fullStr | Zinc Oxide Nanoparticles Induces Apoptosis in Human Breast Cancer Cells via Caspase-8 and P53 Pathway |
| title_full_unstemmed | Zinc Oxide Nanoparticles Induces Apoptosis in Human Breast Cancer Cells via Caspase-8 and P53 Pathway |
| title_short | Zinc Oxide Nanoparticles Induces Apoptosis in Human Breast Cancer Cells via Caspase-8 and P53 Pathway |
| title_sort | zinc oxide nanoparticles induces apoptosis in human breast cancer cells via caspase 8 and p53 pathway |
| topic | zinc oxide nanoparticles cytotoxicity mcf-7 genotoxicity caspase-8 p53 |
| url | https://www.sciopen.com/article/10.5101/nbe.v11i1.p35-43 |
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