Effects of Various Phytohormones On Haploid Wheat Production in Wheat x Maize Crosses
The Effects of Phytohormones On The Production of Haploid Wheat Were Examined in The intergeneric Crosses Between Japanese Wheat Cultivar (Triticum Aestivum Cv. Zenkojikomugi) and Maize (ZEA MAYS Cv. Pioneer P80 Lisa). The Detached Wheat Spikes Pollinated With Maize Were Cultured in A Solution Conta...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2007-01-01
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| Series: | Plant Production Science |
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| Online Access: | http://dx.doi.org/10.1626/pps.10.36 |
| _version_ | 1819061746246090752 |
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| author | Tomohiko Ushiyama Tatsuo Kuwabara Tomohiko Yoshida |
| author_facet | Tomohiko Ushiyama Tatsuo Kuwabara Tomohiko Yoshida |
| author_sort | Tomohiko Ushiyama |
| collection | DOAJ |
| description | The Effects of Phytohormones On The Production of Haploid Wheat Were Examined in The intergeneric Crosses Between Japanese Wheat Cultivar (Triticum Aestivum Cv. Zenkojikomugi) and Maize (ZEA MAYS Cv. Pioneer P80 Lisa). The Detached Wheat Spikes Pollinated With Maize Were Cultured in A Solution Containing Sucrose and Sulfurous Acid Supplemented With 2,4―Dichlorophenoxyacetic Acid (2,4―D), indole-Acetic Acid, Naphthalene Acetic Acid, Kinetin Or 6-Benzylaminopurine At 0, 0.1, 1, 10, 100 Or 1000 Mg L-1. Haploid Embryos Obtained Were Cultured On Agarose-Solidified B5 Medium. The Frequency of Plant Regeneration Was Significantly Affected Only By The Treatment With 100 Mg L-1 2,4―D. Thus, The Detached Spikes Were Cultured in The Medium Containing 2,4―D At 0, 25, 50, 75, 100, 125, 150, 175, 200 Or 400 Mg L-1. The Treatments With 50 Mg L-1 2,4―D increased The Embryo Size, But The Treatments With Above 75 Mg L-1 2,4―D inhibited The Development of The Embryo. The Percentage of Florets Developing into Haploid Plants Was increased By The Treatment With 100 Mg L-1 2,4―D. Therefore, The Concentration of 2,4―D in THe Spikes Treated With 2,4―D At 50, 100 and 150 Mg L-1 WERe Measured By Gas Chromatography. The Concentration of 2,4―D in THE Seed Was increased To 9.24 Ppm By The Treatment With 100 Mg L-1 2,4―D, A Further increase of 2,4―D Concentration in The Medium Having No Effect. On The Other Hand, The Concentration of 2,4―D in The Glumes and Rachis increased Up To 12.72 and 41.55 Ppm By The TreatmenT With 100 and 150 Mg L-1 2,4―D, Respectively. The Treatments With 2,4―D At A Concentration Higher Than 100 Mg L-1 inhibited Embryo Development. The Present Results Suggested That 2,4―D At The Concentrations From 50 To 100 Mg L-1 Would Be Optimum For Haploid Wheat Production Using Maize. |
| first_indexed | 2024-12-21T14:47:47Z |
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| id | doaj.art-eadb3274f17d4c1cb5c6d0ca5675ef86 |
| institution | Directory Open Access Journal |
| issn | 1343-943X 1349-1008 |
| language | English |
| last_indexed | 2024-12-21T14:47:47Z |
| publishDate | 2007-01-01 |
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| spelling | doaj.art-eadb3274f17d4c1cb5c6d0ca5675ef862022-12-21T18:59:59ZengTaylor & Francis GroupPlant Production Science1343-943X1349-10082007-01-01101364110.1626/pps.10.3611644809Effects of Various Phytohormones On Haploid Wheat Production in Wheat x Maize CrossesTomohiko Ushiyama0Tatsuo Kuwabara1Tomohiko Yoshida2United Graduate School of Agricultural Science, Tokyo University of Agriculture and TechnologyNational Agricultural Research Center for Hokkaido RegionFacurty of Agriculture, Utsunomiya UniversityThe Effects of Phytohormones On The Production of Haploid Wheat Were Examined in The intergeneric Crosses Between Japanese Wheat Cultivar (Triticum Aestivum Cv. Zenkojikomugi) and Maize (ZEA MAYS Cv. Pioneer P80 Lisa). The Detached Wheat Spikes Pollinated With Maize Were Cultured in A Solution Containing Sucrose and Sulfurous Acid Supplemented With 2,4―Dichlorophenoxyacetic Acid (2,4―D), indole-Acetic Acid, Naphthalene Acetic Acid, Kinetin Or 6-Benzylaminopurine At 0, 0.1, 1, 10, 100 Or 1000 Mg L-1. Haploid Embryos Obtained Were Cultured On Agarose-Solidified B5 Medium. The Frequency of Plant Regeneration Was Significantly Affected Only By The Treatment With 100 Mg L-1 2,4―D. Thus, The Detached Spikes Were Cultured in The Medium Containing 2,4―D At 0, 25, 50, 75, 100, 125, 150, 175, 200 Or 400 Mg L-1. The Treatments With 50 Mg L-1 2,4―D increased The Embryo Size, But The Treatments With Above 75 Mg L-1 2,4―D inhibited The Development of The Embryo. The Percentage of Florets Developing into Haploid Plants Was increased By The Treatment With 100 Mg L-1 2,4―D. Therefore, The Concentration of 2,4―D in THe Spikes Treated With 2,4―D At 50, 100 and 150 Mg L-1 WERe Measured By Gas Chromatography. The Concentration of 2,4―D in THE Seed Was increased To 9.24 Ppm By The Treatment With 100 Mg L-1 2,4―D, A Further increase of 2,4―D Concentration in The Medium Having No Effect. On The Other Hand, The Concentration of 2,4―D in The Glumes and Rachis increased Up To 12.72 and 41.55 Ppm By The TreatmenT With 100 and 150 Mg L-1 2,4―D, Respectively. The Treatments With 2,4―D At A Concentration Higher Than 100 Mg L-1 inhibited Embryo Development. The Present Results Suggested That 2,4―D At The Concentrations From 50 To 100 Mg L-1 Would Be Optimum For Haploid Wheat Production Using Maize.http://dx.doi.org/10.1626/pps.10.362,4–Dichlorophenoxyacetic acidHaploidintergeneric crossPhytohormonePlant regenerationTriticum aestivumZea mays |
| spellingShingle | Tomohiko Ushiyama Tatsuo Kuwabara Tomohiko Yoshida Effects of Various Phytohormones On Haploid Wheat Production in Wheat x Maize Crosses Plant Production Science 2,4–Dichlorophenoxyacetic acid Haploid intergeneric cross Phytohormone Plant regeneration Triticum aestivum Zea mays |
| title | Effects of Various Phytohormones On Haploid Wheat Production in Wheat x Maize Crosses |
| title_full | Effects of Various Phytohormones On Haploid Wheat Production in Wheat x Maize Crosses |
| title_fullStr | Effects of Various Phytohormones On Haploid Wheat Production in Wheat x Maize Crosses |
| title_full_unstemmed | Effects of Various Phytohormones On Haploid Wheat Production in Wheat x Maize Crosses |
| title_short | Effects of Various Phytohormones On Haploid Wheat Production in Wheat x Maize Crosses |
| title_sort | effects of various phytohormones on haploid wheat production in wheat x maize crosses |
| topic | 2,4–Dichlorophenoxyacetic acid Haploid intergeneric cross Phytohormone Plant regeneration Triticum aestivum Zea mays |
| url | http://dx.doi.org/10.1626/pps.10.36 |
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