Gold Nanoparticles Mediate Improved Detection of β-amyloid Aggregates by Fluorescence
The early detection of the amyloid beta peptide aggregates involved in Alzheimer’s disease is crucial to test new potential treatments. In this research, we improved the detection of amyloid beta peptide aggregates in vitro and ex vivo by fluorescence combining the use of CRANAD-2 and gold nanorods...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-04-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/10/4/690 |
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| author | Pedro Jara-Guajardo Pablo Cabrera Freddy Celis Mónica Soler Isadora Berlanga Nicole Parra-Muñoz Gerardo Acosta Fernando Albericio Fanny Guzman Marcelo Campos Alejandra Alvarez Francisco Morales-Zavala Marcelo J Kogan |
| author_facet | Pedro Jara-Guajardo Pablo Cabrera Freddy Celis Mónica Soler Isadora Berlanga Nicole Parra-Muñoz Gerardo Acosta Fernando Albericio Fanny Guzman Marcelo Campos Alejandra Alvarez Francisco Morales-Zavala Marcelo J Kogan |
| author_sort | Pedro Jara-Guajardo |
| collection | DOAJ |
| description | The early detection of the amyloid beta peptide aggregates involved in Alzheimer’s disease is crucial to test new potential treatments. In this research, we improved the detection of amyloid beta peptide aggregates in vitro and ex vivo by fluorescence combining the use of CRANAD-2 and gold nanorods (GNRs) by the surface enhancement fluorescence effect. We synthetized GNRs and modified their surface with HS-PEG-OMe and HS-PEG-COOH and functionalized them with the D1 peptide, which has the capability to selectively bind to amyloid beta peptide. For an in vitro detection of amyloid beta peptide, we co-incubated amyloid beta peptide aggregates with the probe CRANAD-2 and GNR-PEG-D1 observing an increase in the intensity of the fluorescence signal attributed to surface enhancement fluorescence. Furthermore, the surface enhancement fluorescence effect was observed in brain slices of transgenic mice with Alzheimer´s disease co-incubated with CRANAD-2 and GNR-PEG-D1. An increase in the fluorescence signal was observed allowing the detection of aggregates that cannot be detected with the single use of CRANAD-2. Gold nanoparticles allowed an improvement in the detection of the amyloid aggregated by fluorescence in vitro and ex vivo. |
| first_indexed | 2024-03-10T20:39:20Z |
| format | Article |
| id | doaj.art-eadcecbc5fc741b5a06c194204f30c08 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-10T20:39:20Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-eadcecbc5fc741b5a06c194204f30c082023-11-19T20:49:49ZengMDPI AGNanomaterials2079-49912020-04-0110469010.3390/nano10040690Gold Nanoparticles Mediate Improved Detection of β-amyloid Aggregates by FluorescencePedro Jara-Guajardo0Pablo Cabrera1Freddy Celis2Mónica Soler3Isadora Berlanga4Nicole Parra-Muñoz5Gerardo Acosta6Fernando Albericio7Fanny Guzman8Marcelo Campos9Alejandra Alvarez10Francisco Morales-Zavala11Marcelo J Kogan12Departamento de Química Farmacológica y Toxicológica, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile, Santiago 8380494, ChileDepartamento de Química Farmacológica y Toxicológica, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile, Santiago 8380494, ChileLaboratorio de Procesos Fotónicos y Electroquímicos, Universidad de Playa Ancha, Playa Ancha 850, Valparaíso, ChileDepartamento de Ingeniería Química, Biotecnología y Materiales, Facultad de Ciencias Físicas y Matemáticas, Universidad de Chile, Beaucheff 851, Santiago 8380494, ChileDepartamento de Ingeniería Química, Biotecnología y Materiales, Facultad de Ciencias Físicas y Matemáticas, Universidad de Chile, Beaucheff 851, Santiago 8380494, ChileDepartamento de Ingeniería Química, Biotecnología y Materiales, Facultad de Ciencias Físicas y Matemáticas, Universidad de Chile, Beaucheff 851, Santiago 8380494, ChileCIBER-BBN, Networking Centre on Bioengineering, Biomaterials and Nanomedicine & Department of Organic Chemistry, Marti i Franques 1-11, University of Barcelona (UB), 08028 Barcelona, SpainCIBER-BBN, Networking Centre on Bioengineering, Biomaterials and Nanomedicine & Department of Organic Chemistry, Marti i Franques 1-11, University of Barcelona (UB), 08028 Barcelona, SpainNúcleo de Biotecnología Curauma (NBC), Pontificia Universidad Católica de Valparaíso, Valparaíso 2460355, ChileDepartment of Chemistry, Faculty of Sciences, University of Chile, POBox 653, Santiago 8380494, ChileFacultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Alameda 340, Santiago 8331010, ChileDepartamento de Química Farmacológica y Toxicológica, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile, Santiago 8380494, ChileDepartamento de Química Farmacológica y Toxicológica, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile, Santiago 8380494, ChileThe early detection of the amyloid beta peptide aggregates involved in Alzheimer’s disease is crucial to test new potential treatments. In this research, we improved the detection of amyloid beta peptide aggregates in vitro and ex vivo by fluorescence combining the use of CRANAD-2 and gold nanorods (GNRs) by the surface enhancement fluorescence effect. We synthetized GNRs and modified their surface with HS-PEG-OMe and HS-PEG-COOH and functionalized them with the D1 peptide, which has the capability to selectively bind to amyloid beta peptide. For an in vitro detection of amyloid beta peptide, we co-incubated amyloid beta peptide aggregates with the probe CRANAD-2 and GNR-PEG-D1 observing an increase in the intensity of the fluorescence signal attributed to surface enhancement fluorescence. Furthermore, the surface enhancement fluorescence effect was observed in brain slices of transgenic mice with Alzheimer´s disease co-incubated with CRANAD-2 and GNR-PEG-D1. An increase in the fluorescence signal was observed allowing the detection of aggregates that cannot be detected with the single use of CRANAD-2. Gold nanoparticles allowed an improvement in the detection of the amyloid aggregated by fluorescence in vitro and ex vivo.https://www.mdpi.com/2079-4991/10/4/690gold nanorodsCRANAD-2amyloid beta peptideSEFAlzheimer´s disease |
| spellingShingle | Pedro Jara-Guajardo Pablo Cabrera Freddy Celis Mónica Soler Isadora Berlanga Nicole Parra-Muñoz Gerardo Acosta Fernando Albericio Fanny Guzman Marcelo Campos Alejandra Alvarez Francisco Morales-Zavala Marcelo J Kogan Gold Nanoparticles Mediate Improved Detection of β-amyloid Aggregates by Fluorescence Nanomaterials gold nanorods CRANAD-2 amyloid beta peptide SEF Alzheimer´s disease |
| title | Gold Nanoparticles Mediate Improved Detection of β-amyloid Aggregates by Fluorescence |
| title_full | Gold Nanoparticles Mediate Improved Detection of β-amyloid Aggregates by Fluorescence |
| title_fullStr | Gold Nanoparticles Mediate Improved Detection of β-amyloid Aggregates by Fluorescence |
| title_full_unstemmed | Gold Nanoparticles Mediate Improved Detection of β-amyloid Aggregates by Fluorescence |
| title_short | Gold Nanoparticles Mediate Improved Detection of β-amyloid Aggregates by Fluorescence |
| title_sort | gold nanoparticles mediate improved detection of β amyloid aggregates by fluorescence |
| topic | gold nanorods CRANAD-2 amyloid beta peptide SEF Alzheimer´s disease |
| url | https://www.mdpi.com/2079-4991/10/4/690 |
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