The Role of Hemicellulose in Cadmium Tolerance in Ramie (<i>Boehmeria nivea</i> (L.) Gaud.)
Ramie cell walls play an important role in cadmium (Cd) detoxification. However, the Cd binding capacity of the cell wall components and the cell wall compositions among ramie species remains unclear. Therefore, this study compared two ramie populations (‘Dazhuhuangbaima’ (low-Cd-accumulating popula...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-07-01
|
| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/11/15/1941 |
| _version_ | 1827601939068616704 |
|---|---|
| author | Yushen Ma Hongdong Jie Yanyi Tang Hucheng Xing Yucheng Jie |
| author_facet | Yushen Ma Hongdong Jie Yanyi Tang Hucheng Xing Yucheng Jie |
| author_sort | Yushen Ma |
| collection | DOAJ |
| description | Ramie cell walls play an important role in cadmium (Cd) detoxification. However, the Cd binding capacity of the cell wall components and the cell wall compositions among ramie species remains unclear. Therefore, this study compared two ramie populations (‘Dazhuhuangbaima’ (low-Cd-accumulating population) and ‘Zhongzhu 1’ (high-Cd-accumulating population)) with different Cd enrichment characteristics. The two ramie populations were treated with 0, 25, and 75 mg kg<sup>−1</sup> Cd for 30 days; then, their root length, plant height, biomass, Cd enrichment in the organs, subcellular Cd distribution, Cd content in the cell wall polysaccharides, and hemicellulose content were determined. The root length, plant height, biomass, and Cd enrichment in all organs were significantly higher (<i>p</i> ≤ 0.05) in ‘Zhongzhu 1’ than in ‘Dazhuhuangbaima’ under Cd stress. In addition, the subcellular Cd distribution analysis revealed that Cd was mainly found in the cell wall in both ramie populations. Among the cell wall fractions, Cd was mainly bound to the hemicelluloses, with 60.38–73.10% and 50.05–64.45% Cd accumulating in the ‘Zhongzhu 1’ and ‘Dazhuhuangbaima’ cell wall hemicelluloses, respectively. However, the Cd concentration in the ‘Zhongzhu 1’ hemicellulose was significantly higher (<i>p</i> ≤ 0.05) than that in the ‘Dazhuhuangbaima’ hemicellulose. Hemicellulose content analysis further revealed that the hemicellulose concentration increased with the Cd concentration in both populations, but it was significantly higher (<i>p</i> ≤ 0.05) in ‘Zhongzhu 1’ than in ‘Dazhuhuangbaima’ across all Cd treatments. Thus, ramie copes under Cd stress by increasing the hemicellulose content in the cell wall. The findings in this study confirm that hemicellulose is the main enrichment site for Cd in ramie. It also provides a theoretical basis for Cd enrichment breeding in ramie. |
| first_indexed | 2024-03-09T05:06:31Z |
| format | Article |
| id | doaj.art-eae051a3724844eb966e3067f8f9bc6a |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T05:06:31Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-eae051a3724844eb966e3067f8f9bc6a2023-12-03T12:54:56ZengMDPI AGPlants2223-77472022-07-011115194110.3390/plants11151941The Role of Hemicellulose in Cadmium Tolerance in Ramie (<i>Boehmeria nivea</i> (L.) Gaud.)Yushen Ma0Hongdong Jie1Yanyi Tang2Hucheng Xing3Yucheng Jie4College of Agronomy, Hunan Agricultural University, Changsha 410128, ChinaCollege of Agronomy, Hunan Agricultural University, Changsha 410128, ChinaCollege of Agronomy, Hunan Agricultural University, Changsha 410128, ChinaCollege of Agronomy, Hunan Agricultural University, Changsha 410128, ChinaCollege of Agronomy, Hunan Agricultural University, Changsha 410128, ChinaRamie cell walls play an important role in cadmium (Cd) detoxification. However, the Cd binding capacity of the cell wall components and the cell wall compositions among ramie species remains unclear. Therefore, this study compared two ramie populations (‘Dazhuhuangbaima’ (low-Cd-accumulating population) and ‘Zhongzhu 1’ (high-Cd-accumulating population)) with different Cd enrichment characteristics. The two ramie populations were treated with 0, 25, and 75 mg kg<sup>−1</sup> Cd for 30 days; then, their root length, plant height, biomass, Cd enrichment in the organs, subcellular Cd distribution, Cd content in the cell wall polysaccharides, and hemicellulose content were determined. The root length, plant height, biomass, and Cd enrichment in all organs were significantly higher (<i>p</i> ≤ 0.05) in ‘Zhongzhu 1’ than in ‘Dazhuhuangbaima’ under Cd stress. In addition, the subcellular Cd distribution analysis revealed that Cd was mainly found in the cell wall in both ramie populations. Among the cell wall fractions, Cd was mainly bound to the hemicelluloses, with 60.38–73.10% and 50.05–64.45% Cd accumulating in the ‘Zhongzhu 1’ and ‘Dazhuhuangbaima’ cell wall hemicelluloses, respectively. However, the Cd concentration in the ‘Zhongzhu 1’ hemicellulose was significantly higher (<i>p</i> ≤ 0.05) than that in the ‘Dazhuhuangbaima’ hemicellulose. Hemicellulose content analysis further revealed that the hemicellulose concentration increased with the Cd concentration in both populations, but it was significantly higher (<i>p</i> ≤ 0.05) in ‘Zhongzhu 1’ than in ‘Dazhuhuangbaima’ across all Cd treatments. Thus, ramie copes under Cd stress by increasing the hemicellulose content in the cell wall. The findings in this study confirm that hemicellulose is the main enrichment site for Cd in ramie. It also provides a theoretical basis for Cd enrichment breeding in ramie.https://www.mdpi.com/2223-7747/11/15/1941hemicellulosecadmiumramietolerance |
| spellingShingle | Yushen Ma Hongdong Jie Yanyi Tang Hucheng Xing Yucheng Jie The Role of Hemicellulose in Cadmium Tolerance in Ramie (<i>Boehmeria nivea</i> (L.) Gaud.) Plants hemicellulose cadmium ramie tolerance |
| title | The Role of Hemicellulose in Cadmium Tolerance in Ramie (<i>Boehmeria nivea</i> (L.) Gaud.) |
| title_full | The Role of Hemicellulose in Cadmium Tolerance in Ramie (<i>Boehmeria nivea</i> (L.) Gaud.) |
| title_fullStr | The Role of Hemicellulose in Cadmium Tolerance in Ramie (<i>Boehmeria nivea</i> (L.) Gaud.) |
| title_full_unstemmed | The Role of Hemicellulose in Cadmium Tolerance in Ramie (<i>Boehmeria nivea</i> (L.) Gaud.) |
| title_short | The Role of Hemicellulose in Cadmium Tolerance in Ramie (<i>Boehmeria nivea</i> (L.) Gaud.) |
| title_sort | role of hemicellulose in cadmium tolerance in ramie i boehmeria nivea i l gaud |
| topic | hemicellulose cadmium ramie tolerance |
| url | https://www.mdpi.com/2223-7747/11/15/1941 |
| work_keys_str_mv | AT yushenma theroleofhemicelluloseincadmiumtoleranceinramieiboehmerianiveailgaud AT hongdongjie theroleofhemicelluloseincadmiumtoleranceinramieiboehmerianiveailgaud AT yanyitang theroleofhemicelluloseincadmiumtoleranceinramieiboehmerianiveailgaud AT huchengxing theroleofhemicelluloseincadmiumtoleranceinramieiboehmerianiveailgaud AT yuchengjie theroleofhemicelluloseincadmiumtoleranceinramieiboehmerianiveailgaud AT yushenma roleofhemicelluloseincadmiumtoleranceinramieiboehmerianiveailgaud AT hongdongjie roleofhemicelluloseincadmiumtoleranceinramieiboehmerianiveailgaud AT yanyitang roleofhemicelluloseincadmiumtoleranceinramieiboehmerianiveailgaud AT huchengxing roleofhemicelluloseincadmiumtoleranceinramieiboehmerianiveailgaud AT yuchengjie roleofhemicelluloseincadmiumtoleranceinramieiboehmerianiveailgaud |