Comparative evaluation of serological tests in the diagnosis of Syphilis
Background: Diagnostic and therapeutic approaches in syphilis show wide variation. The use of only one type of serological test is insufficient for diagnosis. Objective: The aim of the study was to review serologic data to determine diagnostic performance of three different methods of syphilis...
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| Format: | Article |
| Language: | English |
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Prathima Institute of Medical Sciences
2017-04-01
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| Series: | Perspectives In Medical Research |
| Subjects: | |
| Online Access: | http://www.pimr.org.in/Sithamahalaxmi.PDF |
| _version_ | 1797462419762577408 |
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| author | Sita Mahalakshmi B1 , Sreedevi S2 , Jyothi K3 |
| author_facet | Sita Mahalakshmi B1 , Sreedevi S2 , Jyothi K3 |
| author_sort | Sita Mahalakshmi B1 , Sreedevi S2 , Jyothi K3 |
| collection | DOAJ |
| description | Background: Diagnostic and therapeutic approaches in
syphilis show wide variation. The use of only one type of
serological test is insufficient for diagnosis.
Objective: The aim of the study was to review serologic
data to determine diagnostic performance of three different
methods of syphilis patients from MGM Hospital, Warangal in
urban and rural areas during period of 2012-2013.
Materials & Methods: In total, 144 RPR reactive
samples were collected, with 1 in 4 or more titre of antibody
by using RPR, TRUST and TPHA assays
Results: 144 test sera were collected from 3 sources
namely ICTC, STD and Antenatal OP. 70 sera (48.6%) were taken
from ICTC, 62 (43.5%) from STD and 12 (8.3%) from antenatal
OP. Table compares the sensitivity and titres of antibody in
RPR, TPHA and TRUST. Maximum number of samples (68) are
reactive at 1:8 dilutions by RPR, 320 dilutions in TPHA and 64
dilutions in TRUST. The reactivity of all the samples by TPHA is
80 and above, and by TRUST, 32 and above.
Conclusion: Based on our results we think that specific
Treponemal tests could contribute to reducing the errors that
depend on specificity of the method used. Considering the
methodology, rapid results and high sensitivity of RPR tests
makes it a good choice as screening test in microbiology
laboratories |
| first_indexed | 2024-03-09T17:36:16Z |
| format | Article |
| id | doaj.art-eae23486df3f41fb91e02a5d47799d40 |
| institution | Directory Open Access Journal |
| issn | 2348-1447 2348-229X |
| language | English |
| last_indexed | 2024-03-09T17:36:16Z |
| publishDate | 2017-04-01 |
| publisher | Prathima Institute of Medical Sciences |
| record_format | Article |
| series | Perspectives In Medical Research |
| spelling | doaj.art-eae23486df3f41fb91e02a5d47799d402023-11-24T11:52:43ZengPrathima Institute of Medical SciencesPerspectives In Medical Research2348-14472348-229X2017-04-01515054Comparative evaluation of serological tests in the diagnosis of SyphilisSita Mahalakshmi B1 , Sreedevi S2 , Jyothi K30Kakatiya Medical CollegeBackground: Diagnostic and therapeutic approaches in syphilis show wide variation. The use of only one type of serological test is insufficient for diagnosis. Objective: The aim of the study was to review serologic data to determine diagnostic performance of three different methods of syphilis patients from MGM Hospital, Warangal in urban and rural areas during period of 2012-2013. Materials & Methods: In total, 144 RPR reactive samples were collected, with 1 in 4 or more titre of antibody by using RPR, TRUST and TPHA assays Results: 144 test sera were collected from 3 sources namely ICTC, STD and Antenatal OP. 70 sera (48.6%) were taken from ICTC, 62 (43.5%) from STD and 12 (8.3%) from antenatal OP. Table compares the sensitivity and titres of antibody in RPR, TPHA and TRUST. Maximum number of samples (68) are reactive at 1:8 dilutions by RPR, 320 dilutions in TPHA and 64 dilutions in TRUST. The reactivity of all the samples by TPHA is 80 and above, and by TRUST, 32 and above. Conclusion: Based on our results we think that specific Treponemal tests could contribute to reducing the errors that depend on specificity of the method used. Considering the methodology, rapid results and high sensitivity of RPR tests makes it a good choice as screening test in microbiology laboratorieshttp://www.pimr.org.in/Sithamahalaxmi.PDFsyphilistreponema pallidumrpr and tpha |
| spellingShingle | Sita Mahalakshmi B1 , Sreedevi S2 , Jyothi K3 Comparative evaluation of serological tests in the diagnosis of Syphilis Perspectives In Medical Research syphilis treponema pallidum rpr and tpha |
| title | Comparative evaluation of serological tests in the diagnosis of Syphilis |
| title_full | Comparative evaluation of serological tests in the diagnosis of Syphilis |
| title_fullStr | Comparative evaluation of serological tests in the diagnosis of Syphilis |
| title_full_unstemmed | Comparative evaluation of serological tests in the diagnosis of Syphilis |
| title_short | Comparative evaluation of serological tests in the diagnosis of Syphilis |
| title_sort | comparative evaluation of serological tests in the diagnosis of syphilis |
| topic | syphilis treponema pallidum rpr and tpha |
| url | http://www.pimr.org.in/Sithamahalaxmi.PDF |
| work_keys_str_mv | AT sitamahalakshmib1sreedevis2jyothik3 comparativeevaluationofserologicaltestsinthediagnosisofsyphilis |