Genetic Diversity of the Hepatitis B Virus Subgenotypes in Brazil
Hepatitis B virus (HBV) subgenotypes may be related to clinical outcomes and response to antiviral therapy. Most Brazilian studies on HBV subgenotypes are restricted to some regions and to specific population groups. Here, we provide an insight about genetic diversity of HBV subgenotypes in 321 seru...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2019-09-01
|
| Series: | Viruses |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4915/11/9/860 |
| _version_ | 1818286998560964608 |
|---|---|
| author | Barbara V. Lago Marcia P. do Espirito-Santo Vanessa D. Costa Vanessa A. Marques Livia M. Villar Lia L. Lewis-Ximenez Elisabeth Lampe Francisco C. A. Mello |
| author_facet | Barbara V. Lago Marcia P. do Espirito-Santo Vanessa D. Costa Vanessa A. Marques Livia M. Villar Lia L. Lewis-Ximenez Elisabeth Lampe Francisco C. A. Mello |
| author_sort | Barbara V. Lago |
| collection | DOAJ |
| description | Hepatitis B virus (HBV) subgenotypes may be related to clinical outcomes and response to antiviral therapy. Most Brazilian studies on HBV subgenotypes are restricted to some regions and to specific population groups. Here, we provide an insight about genetic diversity of HBV subgenotypes in 321 serum samples from all five geographical regions, providing a representative overview of their circulation among chronic carriers. Overall, HBV/A1 was the most prevalent subgenotype, being found as the major one in all regions except in South Brazil. Among HBV/D samples, subgenotype D3 was the most prevalent, found in 51.5%, followed by D2 (27.3%) and D4 (21.2%). D2 and D3 were the most prevalent subgenotypes in South region, with high similarity with European strains. D4 was found in North and Northeast region and clustered with strains from Cape Verde and India. For HBV/F, the most frequent subgenotype was F2 (84.1%), followed by F4 (10.1%) and F1 (5.8%), closely related with strains from Venezuela, Argentina and Chile, respectively. Phylogeographic analyses were performed using an HBV full-length genome obtained from samples infected with genotypes rarely found in Brazil (B, C, and E). According to Bayesian inference, HBV/B2 and HBV/C2 were probably introduced in Brazil through China, and HBV/E from Guinea, all of them mostly linked to recent events of human migration. In conclusion, this study provided a comprehensive overview of the current circulation of HBV subgenotypes in Brazil. Our findings might contribute to a better understand of the dynamics of viral variants, to establish a permanent molecular surveillance on the introduction and dispersion patterns of new strains and, thus, to support public policies to control HBV dissemination in Brazil. |
| first_indexed | 2024-12-13T01:33:30Z |
| format | Article |
| id | doaj.art-eae315cab7644ec99cb3c0693ebc75ba |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-12-13T01:33:30Z |
| publishDate | 2019-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-eae315cab7644ec99cb3c0693ebc75ba2022-12-22T00:03:56ZengMDPI AGViruses1999-49152019-09-0111986010.3390/v11090860v11090860Genetic Diversity of the Hepatitis B Virus Subgenotypes in BrazilBarbara V. Lago0Marcia P. do Espirito-Santo1Vanessa D. Costa2Vanessa A. Marques3Livia M. Villar4Lia L. Lewis-Ximenez5Elisabeth Lampe6Francisco C. A. Mello7Laboratório de Hepatites Virais, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, RJ 21040-900, BrazilInstituto de Tecnologia em Imunobiológicos (Bio-Manguinhos), FIOCRUZ, Rio de Janeiro, RJ 21040-900, BrazilLaboratório de Hepatites Virais, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, RJ 21040-900, BrazilLaboratório de Hepatites Virais, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, RJ 21040-900, BrazilLaboratório de Hepatites Virais, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, RJ 21040-900, BrazilLaboratório de Hepatites Virais, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, RJ 21040-900, BrazilLaboratório de Hepatites Virais, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, RJ 21040-900, BrazilLaboratório de Hepatites Virais, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, RJ 21040-900, BrazilHepatitis B virus (HBV) subgenotypes may be related to clinical outcomes and response to antiviral therapy. Most Brazilian studies on HBV subgenotypes are restricted to some regions and to specific population groups. Here, we provide an insight about genetic diversity of HBV subgenotypes in 321 serum samples from all five geographical regions, providing a representative overview of their circulation among chronic carriers. Overall, HBV/A1 was the most prevalent subgenotype, being found as the major one in all regions except in South Brazil. Among HBV/D samples, subgenotype D3 was the most prevalent, found in 51.5%, followed by D2 (27.3%) and D4 (21.2%). D2 and D3 were the most prevalent subgenotypes in South region, with high similarity with European strains. D4 was found in North and Northeast region and clustered with strains from Cape Verde and India. For HBV/F, the most frequent subgenotype was F2 (84.1%), followed by F4 (10.1%) and F1 (5.8%), closely related with strains from Venezuela, Argentina and Chile, respectively. Phylogeographic analyses were performed using an HBV full-length genome obtained from samples infected with genotypes rarely found in Brazil (B, C, and E). According to Bayesian inference, HBV/B2 and HBV/C2 were probably introduced in Brazil through China, and HBV/E from Guinea, all of them mostly linked to recent events of human migration. In conclusion, this study provided a comprehensive overview of the current circulation of HBV subgenotypes in Brazil. Our findings might contribute to a better understand of the dynamics of viral variants, to establish a permanent molecular surveillance on the introduction and dispersion patterns of new strains and, thus, to support public policies to control HBV dissemination in Brazil.https://www.mdpi.com/1999-4915/11/9/860hepatitis B virussubgenotypesBrazil |
| spellingShingle | Barbara V. Lago Marcia P. do Espirito-Santo Vanessa D. Costa Vanessa A. Marques Livia M. Villar Lia L. Lewis-Ximenez Elisabeth Lampe Francisco C. A. Mello Genetic Diversity of the Hepatitis B Virus Subgenotypes in Brazil Viruses hepatitis B virus subgenotypes Brazil |
| title | Genetic Diversity of the Hepatitis B Virus Subgenotypes in Brazil |
| title_full | Genetic Diversity of the Hepatitis B Virus Subgenotypes in Brazil |
| title_fullStr | Genetic Diversity of the Hepatitis B Virus Subgenotypes in Brazil |
| title_full_unstemmed | Genetic Diversity of the Hepatitis B Virus Subgenotypes in Brazil |
| title_short | Genetic Diversity of the Hepatitis B Virus Subgenotypes in Brazil |
| title_sort | genetic diversity of the hepatitis b virus subgenotypes in brazil |
| topic | hepatitis B virus subgenotypes Brazil |
| url | https://www.mdpi.com/1999-4915/11/9/860 |
| work_keys_str_mv | AT barbaravlago geneticdiversityofthehepatitisbvirussubgenotypesinbrazil AT marciapdoespiritosanto geneticdiversityofthehepatitisbvirussubgenotypesinbrazil AT vanessadcosta geneticdiversityofthehepatitisbvirussubgenotypesinbrazil AT vanessaamarques geneticdiversityofthehepatitisbvirussubgenotypesinbrazil AT liviamvillar geneticdiversityofthehepatitisbvirussubgenotypesinbrazil AT liallewisximenez geneticdiversityofthehepatitisbvirussubgenotypesinbrazil AT elisabethlampe geneticdiversityofthehepatitisbvirussubgenotypesinbrazil AT franciscocamello geneticdiversityofthehepatitisbvirussubgenotypesinbrazil |