Micropropagation and Microrhizome Induction in Costus pictus D. Don Using In Vitro and Ex Vitro Nodal Segments as Explant
Costus pictus D. Don is a potent anti-diabetic plant used in folk, ayurvedic and homeopathic system of medicine. Gene and protein expression of key targets in insulin signaling pathway have revealed that methyl tetracosonoate, a bio-active molecule from Costus pictus extract has anti-diabetic activi...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Society of Land Measurements and Cadastre from Transylvania (SMTCT)
2012-05-01
|
| Series: | Notulae Scientia Biologicae |
| Online Access: | http://notulaebiologicae.ro/index.php/nsb/article/view/7303 |
| _version_ | 1818012501145550848 |
|---|---|
| author | Kshetrimayum PUNYARANI Jitendra G. SHARMA |
| author_facet | Kshetrimayum PUNYARANI Jitendra G. SHARMA |
| author_sort | Kshetrimayum PUNYARANI |
| collection | DOAJ |
| description | Costus pictus D. Don is a potent anti-diabetic plant used in folk, ayurvedic and homeopathic system of medicine. Gene and protein expression of key targets in insulin signaling pathway have revealed that methyl tetracosonoate, a bio-active molecule from Costus pictus extract has anti-diabetic activity. The axillary buds of Costus pictus are dormant. The dormancy of axillary buds were broken when cultured in Murashige and Skoog (MS) medium supplemented with 3-4 ?M 6-benzylaminopurine (BAP) in combination with 0.2-1 ?M naphthalene acetic acid (NAA). The highest bud-break percentage was achieved in those supplemented with 0.6 ?M NAA and 3 ?M BAP. The sprouted axillary buds were transferred onto medium supplemented with 0.6 ?M NAA and 6-10 ?M BAP for shoot multiplication. The maximum average number of shoot was achieved in medium supplemented with 0.6 ?M NAA and 8 ?M BAP. The shoots were successfully rooted when transferred onto media supplemented with 1-12 ?M NAA or indole-3-acetic acid (IAA) and 3 ?M BAP. The maximum number of roots was found in 8 ?M NAA and 3 ?M BAP. The dormancy of in vitro axillary buds were also successfully broken in stems from which shoot apex were decapitated and cultured in MS medium with 0.6 ?M NAA, 7 ?M BAP and 5-13% sucrose. These sprouted in vitro axillary buds could be used as secondary explants for shoot multiplication. The maximum was in medium supplemented with 9% sucrose. Rhizomes were successfully induced when 4-month old plantlets were cultured on � strength MS medium supplemented with 2.4 ?M NAA, 32 ?M BAP and 5-13% sucrose. Microrhizomes formed in 9% sucrose was largest in size with highest average fresh weight. |
| first_indexed | 2024-04-14T06:20:59Z |
| format | Article |
| id | doaj.art-eae4f2d9f8074f9088aea78452810c03 |
| institution | Directory Open Access Journal |
| issn | 2067-3205 2067-3264 |
| language | English |
| last_indexed | 2024-04-14T06:20:59Z |
| publishDate | 2012-05-01 |
| publisher | Society of Land Measurements and Cadastre from Transylvania (SMTCT) |
| record_format | Article |
| series | Notulae Scientia Biologicae |
| spelling | doaj.art-eae4f2d9f8074f9088aea78452810c032022-12-22T02:08:02ZengSociety of Land Measurements and Cadastre from Transylvania (SMTCT)Notulae Scientia Biologicae2067-32052067-32642012-05-014272787093Micropropagation and Microrhizome Induction in Costus pictus D. Don Using In Vitro and Ex Vitro Nodal Segments as ExplantKshetrimayum PUNYARANI0Jitendra G. SHARMA1Manipur University, Department of Life Sciences, Imphal-795003Manipur University, Department of Life Sciences, Imphal-795003Costus pictus D. Don is a potent anti-diabetic plant used in folk, ayurvedic and homeopathic system of medicine. Gene and protein expression of key targets in insulin signaling pathway have revealed that methyl tetracosonoate, a bio-active molecule from Costus pictus extract has anti-diabetic activity. The axillary buds of Costus pictus are dormant. The dormancy of axillary buds were broken when cultured in Murashige and Skoog (MS) medium supplemented with 3-4 ?M 6-benzylaminopurine (BAP) in combination with 0.2-1 ?M naphthalene acetic acid (NAA). The highest bud-break percentage was achieved in those supplemented with 0.6 ?M NAA and 3 ?M BAP. The sprouted axillary buds were transferred onto medium supplemented with 0.6 ?M NAA and 6-10 ?M BAP for shoot multiplication. The maximum average number of shoot was achieved in medium supplemented with 0.6 ?M NAA and 8 ?M BAP. The shoots were successfully rooted when transferred onto media supplemented with 1-12 ?M NAA or indole-3-acetic acid (IAA) and 3 ?M BAP. The maximum number of roots was found in 8 ?M NAA and 3 ?M BAP. The dormancy of in vitro axillary buds were also successfully broken in stems from which shoot apex were decapitated and cultured in MS medium with 0.6 ?M NAA, 7 ?M BAP and 5-13% sucrose. These sprouted in vitro axillary buds could be used as secondary explants for shoot multiplication. The maximum was in medium supplemented with 9% sucrose. Rhizomes were successfully induced when 4-month old plantlets were cultured on � strength MS medium supplemented with 2.4 ?M NAA, 32 ?M BAP and 5-13% sucrose. Microrhizomes formed in 9% sucrose was largest in size with highest average fresh weight.http://notulaebiologicae.ro/index.php/nsb/article/view/7303 |
| spellingShingle | Kshetrimayum PUNYARANI Jitendra G. SHARMA Micropropagation and Microrhizome Induction in Costus pictus D. Don Using In Vitro and Ex Vitro Nodal Segments as Explant Notulae Scientia Biologicae |
| title | Micropropagation and Microrhizome Induction in Costus pictus D. Don Using In Vitro and Ex Vitro Nodal Segments as Explant |
| title_full | Micropropagation and Microrhizome Induction in Costus pictus D. Don Using In Vitro and Ex Vitro Nodal Segments as Explant |
| title_fullStr | Micropropagation and Microrhizome Induction in Costus pictus D. Don Using In Vitro and Ex Vitro Nodal Segments as Explant |
| title_full_unstemmed | Micropropagation and Microrhizome Induction in Costus pictus D. Don Using In Vitro and Ex Vitro Nodal Segments as Explant |
| title_short | Micropropagation and Microrhizome Induction in Costus pictus D. Don Using In Vitro and Ex Vitro Nodal Segments as Explant |
| title_sort | micropropagation and microrhizome induction in costus pictus d don using in vitro and ex vitro nodal segments as explant |
| url | http://notulaebiologicae.ro/index.php/nsb/article/view/7303 |
| work_keys_str_mv | AT kshetrimayumpunyarani micropropagationandmicrorhizomeinductionincostuspictusddonusinginvitroandexvitronodalsegmentsasexplant AT jitendragsharma micropropagationandmicrorhizomeinductionincostuspictusddonusinginvitroandexvitronodalsegmentsasexplant |