Potential Simultaneous Inhibitors of Angiotensin-Converting Enzyme 2 and Transmembrane Protease, Serine 2
Outbreak of coronavirus disease 2019 occurred in Wuhan and has rapidly spread to almost all parts of world. GB-1, the herbal formula from Tian Shang Sheng Mu of Chiayi Puzi Peitian Temple, is used for the prophylaxis of SARS-CoV-2 in Taiwan. In this study, we investigated that the effect of GB-1 and...
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Frontiers Media S.A.
2020-12-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2020.584158/full |
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| author | Ching-Yuan Wu Ching-Yuan Wu Yu-Shih Lin Yao-Hsu Yang Yao-Hsu Yang Yao-Hsu Yang Li-Hsin Shu Yu-Ching Cheng Hung Te Liu |
| author_facet | Ching-Yuan Wu Ching-Yuan Wu Yu-Shih Lin Yao-Hsu Yang Yao-Hsu Yang Yao-Hsu Yang Li-Hsin Shu Yu-Ching Cheng Hung Te Liu |
| author_sort | Ching-Yuan Wu |
| collection | DOAJ |
| description | Outbreak of coronavirus disease 2019 occurred in Wuhan and has rapidly spread to almost all parts of world. GB-1, the herbal formula from Tian Shang Sheng Mu of Chiayi Puzi Peitian Temple, is used for the prophylaxis of SARS-CoV-2 in Taiwan. In this study, we investigated that the effect of GB-1 and the index compounds of GB-1 on the ACE2 and TMPRSS2 expression through in vitro and in vivo study. In our result, GB-1 can inhibit ACE2 and TMPRSS2 protein expression in HepG2 cells, 293T cells, and Caco-2 cells without cytotoxicity. For the mouse model, GB-1 treatment could decrease ACE2 and TMPRSS2 expression levels of the lung and kidney tissue without adverse effects, including nephrotoxicity and hepatotoxicity. In the compositions of GB-1, 0.5–1 mg/ml of Glycyrrhiza uralensis Fisch. ex DC. extract could not inhibit ACE2 mRNA and protein expression in HepG2 cells. In addition, theaflavin-3-gallate could inhibit protein expression of ACE2 and TMPRSS2 without significant cytotoxicity. Our results suggest that GB-1 and theaflavin-3-gallate could act as potential candidates for prophylaxis or treatment of SARS-CoV-2 infection through inhibiting protein expression of ACE2 and TMPRSS2 for the further study. |
| first_indexed | 2024-12-21T19:15:57Z |
| format | Article |
| id | doaj.art-eae70c415fd545aaa00db5e194ec7c0a |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-12-21T19:15:57Z |
| publishDate | 2020-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-eae70c415fd545aaa00db5e194ec7c0a2022-12-21T18:53:04ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122020-12-011110.3389/fphar.2020.584158584158Potential Simultaneous Inhibitors of Angiotensin-Converting Enzyme 2 and Transmembrane Protease, Serine 2Ching-Yuan Wu0Ching-Yuan Wu1Yu-Shih Lin2Yao-Hsu Yang3Yao-Hsu Yang4Yao-Hsu Yang5Li-Hsin Shu6Yu-Ching Cheng7Hung Te Liu8Department of Traditional Chinese Medicine, Chang Gung Memorial Hospital, Chiayi, TaiwanSchool of Chinese Medicine, College of Medicine, Chang Gung University, Chiayi, TaiwanDepartment of Pharmacy, Chiayi Chang Gung Memorial Hospital, Chiayi, TaiwanDepartment of Traditional Chinese Medicine, Chang Gung Memorial Hospital, Chiayi, TaiwanSchool of Chinese Medicine, College of Medicine, Chang Gung University, Chiayi, TaiwanHealth Information and Epidemiology Laboratory of Chang Gung Memorial Hospital, Chiayi, TaiwanDepartment of Traditional Chinese Medicine, Chang Gung Memorial Hospital, Chiayi, TaiwanDepartment of Traditional Chinese Medicine, Chang Gung Memorial Hospital, Chiayi, TaiwanDepartment of Traditional Chinese Medicine, Chang Gung Memorial Hospital, Chiayi, TaiwanOutbreak of coronavirus disease 2019 occurred in Wuhan and has rapidly spread to almost all parts of world. GB-1, the herbal formula from Tian Shang Sheng Mu of Chiayi Puzi Peitian Temple, is used for the prophylaxis of SARS-CoV-2 in Taiwan. In this study, we investigated that the effect of GB-1 and the index compounds of GB-1 on the ACE2 and TMPRSS2 expression through in vitro and in vivo study. In our result, GB-1 can inhibit ACE2 and TMPRSS2 protein expression in HepG2 cells, 293T cells, and Caco-2 cells without cytotoxicity. For the mouse model, GB-1 treatment could decrease ACE2 and TMPRSS2 expression levels of the lung and kidney tissue without adverse effects, including nephrotoxicity and hepatotoxicity. In the compositions of GB-1, 0.5–1 mg/ml of Glycyrrhiza uralensis Fisch. ex DC. extract could not inhibit ACE2 mRNA and protein expression in HepG2 cells. In addition, theaflavin-3-gallate could inhibit protein expression of ACE2 and TMPRSS2 without significant cytotoxicity. Our results suggest that GB-1 and theaflavin-3-gallate could act as potential candidates for prophylaxis or treatment of SARS-CoV-2 infection through inhibiting protein expression of ACE2 and TMPRSS2 for the further study.https://www.frontiersin.org/articles/10.3389/fphar.2020.584158/fullSARS-CoV-2ACE2TMPRSStheaflavin 3-gallatetheaflavin(+)-catechin 4 |
| spellingShingle | Ching-Yuan Wu Ching-Yuan Wu Yu-Shih Lin Yao-Hsu Yang Yao-Hsu Yang Yao-Hsu Yang Li-Hsin Shu Yu-Ching Cheng Hung Te Liu Potential Simultaneous Inhibitors of Angiotensin-Converting Enzyme 2 and Transmembrane Protease, Serine 2 Frontiers in Pharmacology SARS-CoV-2 ACE2 TMPRSS theaflavin 3-gallate theaflavin (+)-catechin 4 |
| title | Potential Simultaneous Inhibitors of Angiotensin-Converting Enzyme 2 and Transmembrane Protease, Serine 2 |
| title_full | Potential Simultaneous Inhibitors of Angiotensin-Converting Enzyme 2 and Transmembrane Protease, Serine 2 |
| title_fullStr | Potential Simultaneous Inhibitors of Angiotensin-Converting Enzyme 2 and Transmembrane Protease, Serine 2 |
| title_full_unstemmed | Potential Simultaneous Inhibitors of Angiotensin-Converting Enzyme 2 and Transmembrane Protease, Serine 2 |
| title_short | Potential Simultaneous Inhibitors of Angiotensin-Converting Enzyme 2 and Transmembrane Protease, Serine 2 |
| title_sort | potential simultaneous inhibitors of angiotensin converting enzyme 2 and transmembrane protease serine 2 |
| topic | SARS-CoV-2 ACE2 TMPRSS theaflavin 3-gallate theaflavin (+)-catechin 4 |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2020.584158/full |
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