Application of loofa sponge (Luffa cylindrica) as carrier for invertase immobilization for invert sugar syrup production

Invertase immobilization in loofa sponge (Luffa cylindrica) for invert sugar syrup production has been carried out. Loofa sponge was treated with NaIO4 followed by the use of diamine compound, glutaraldehyde and enzyme immobilization. The best results obtained were: invertase immobilized activity: 4...

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Main Authors: Doumit Camilios Neto, João Batista Buzato, Maria Antonia Pedrine Colabone Celligoi, Evandro Cesar Poças
Format: Article
Language:English
Published: Universidade Estadual de Londrina 2004-01-01
Series:Semina: Ciências Exatas e Tecnológicas
Subjects:
Online Access:http://www.uel.br/revistas/uel/index.php/semexatas/article/view/1560
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author Doumit Camilios Neto
João Batista Buzato
Maria Antonia Pedrine Colabone Celligoi
Evandro Cesar Poças
author_facet Doumit Camilios Neto
João Batista Buzato
Maria Antonia Pedrine Colabone Celligoi
Evandro Cesar Poças
author_sort Doumit Camilios Neto
collection DOAJ
description Invertase immobilization in loofa sponge (Luffa cylindrica) for invert sugar syrup production has been carried out. Loofa sponge was treated with NaIO4 followed by the use of diamine compound, glutaraldehyde and enzyme immobilization. The best results obtained were: invertase immobilized activity: 4.510 (mmols/g matrix x min x mL); enzyme coupling efficiency: 0.428%, recovered activity: 1.5% and the extent of hydrolysis was of 2.6x10-3%.h-1.
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spelling doaj.art-eae844604d5243c494e2f2fc79ba6c552022-12-21T21:52:19ZengUniversidade Estadual de LondrinaSemina: Ciências Exatas e Tecnológicas1676-54511679-03752004-01-012515358Application of loofa sponge (Luffa cylindrica) as carrier for invertase immobilization for invert sugar syrup productionDoumit Camilios NetoJoão Batista BuzatoMaria Antonia Pedrine Colabone CelligoiEvandro Cesar PoçasInvertase immobilization in loofa sponge (Luffa cylindrica) for invert sugar syrup production has been carried out. Loofa sponge was treated with NaIO4 followed by the use of diamine compound, glutaraldehyde and enzyme immobilization. The best results obtained were: invertase immobilized activity: 4.510 (mmols/g matrix x min x mL); enzyme coupling efficiency: 0.428%, recovered activity: 1.5% and the extent of hydrolysis was of 2.6x10-3%.h-1.http://www.uel.br/revistas/uel/index.php/semexatas/article/view/1560ImobilizaçãoInvertaseLuffa Cylindrica.
spellingShingle Doumit Camilios Neto
João Batista Buzato
Maria Antonia Pedrine Colabone Celligoi
Evandro Cesar Poças
Application of loofa sponge (Luffa cylindrica) as carrier for invertase immobilization for invert sugar syrup production
Semina: Ciências Exatas e Tecnológicas
Imobilização
Invertase
Luffa Cylindrica.
title Application of loofa sponge (Luffa cylindrica) as carrier for invertase immobilization for invert sugar syrup production
title_full Application of loofa sponge (Luffa cylindrica) as carrier for invertase immobilization for invert sugar syrup production
title_fullStr Application of loofa sponge (Luffa cylindrica) as carrier for invertase immobilization for invert sugar syrup production
title_full_unstemmed Application of loofa sponge (Luffa cylindrica) as carrier for invertase immobilization for invert sugar syrup production
title_short Application of loofa sponge (Luffa cylindrica) as carrier for invertase immobilization for invert sugar syrup production
title_sort application of loofa sponge luffa cylindrica as carrier for invertase immobilization for invert sugar syrup production
topic Imobilização
Invertase
Luffa Cylindrica.
url http://www.uel.br/revistas/uel/index.php/semexatas/article/view/1560
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