Tandem application of endophytic fungus Serendipita indica and phosphorus synergistically recuperate arsenic induced stress in rice
In the context of eco-sustainable acquisition of food security, arsenic (As) acts as a deterring factor, which easily infiltrates our food chain via plant uptake. Therefore, devising climate-smart strategies becomes exigent for minimizing the imposed risks. Pertinently, Serendipita indica (S. indica...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.982668/full |
| _version_ | 1811210995867058176 |
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| author | Shafaque Sehar Qidong Feng Muhammad Faheem Adil Falak Sehar Sahito Zakir Ibrahim Zakir Ibrahim Dost Muhammad Baloch Najeeb Ullah Younan Ouyang Yushuang Guo Imran Haider Shamsi |
| author_facet | Shafaque Sehar Qidong Feng Muhammad Faheem Adil Falak Sehar Sahito Zakir Ibrahim Zakir Ibrahim Dost Muhammad Baloch Najeeb Ullah Younan Ouyang Yushuang Guo Imran Haider Shamsi |
| author_sort | Shafaque Sehar |
| collection | DOAJ |
| description | In the context of eco-sustainable acquisition of food security, arsenic (As) acts as a deterring factor, which easily infiltrates our food chain via plant uptake. Therefore, devising climate-smart strategies becomes exigent for minimizing the imposed risks. Pertinently, Serendipita indica (S. indica) is well reputed for its post-symbiotic stress alleviatory and phyto-promotive potential. Management of phosphorus (P) is acclaimed for mitigating arsenic toxicity in plants by inhibiting the uptake of As molecules due to the competitive cationic exchange in the rhizosphere. The current study was designed to investigate the tandem effects of S. indica and P in combating As toxicity employing two rice genotypes, i.e., Guodao-6 (GD-6; As-sensitive genotype) and Zhongzhe You-1 (ZZY-1; As-tolerant genotype). After successful fungal colonization, alone and combined arsenic (10 μ M L−1) and phosphorus (50 μ M L−1) treatments were applied. Results displayed that the recuperating effects of combined S. indica and P treatment were indeed much profound than their alone treatments; however, most of the beneficial influences were harnessed by ZZY-1 in comparison with GD-6. Distinct genotypic differences were observed for antioxidant enzyme activities, which were induced slightly higher in S. indica-colonized ZZY-1 plants, with or without additional P, as compared to GD-6. Ultrastructure images of root and shoot exhibited ravages of As in the form of chloroplasts-, nuclei-and cell wall-damage with enlarged vacuole area, mellowed mostly by the combined treatment of S. indica and P in both genotypes. Gene expression of PHTs family transporters was regulated at different levels in almost all treatments across genotypes. Conclusively, the results of this study validated the promising role of S. indica and additional P in mitigating As stress, albeit corroborated that the extent of relevant benefit exploitation is highly genotype-dependent. Verily, unlocking the potential of nature-friendly solutions will mend the anthropogenic damage already been done to our environment. |
| first_indexed | 2024-04-12T05:05:27Z |
| format | Article |
| id | doaj.art-eae99a946ecb42d6ad85c43e41723079 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-12T05:05:27Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-eae99a946ecb42d6ad85c43e417230792022-12-22T03:46:53ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-09-011310.3389/fpls.2022.982668982668Tandem application of endophytic fungus Serendipita indica and phosphorus synergistically recuperate arsenic induced stress in riceShafaque Sehar0Qidong Feng1Muhammad Faheem Adil2Falak Sehar Sahito3Zakir Ibrahim4Zakir Ibrahim5Dost Muhammad Baloch6Najeeb Ullah7Younan Ouyang8Yushuang Guo9Imran Haider Shamsi10Zhejiang Key Laboratory of Crop Germplasm Resource, Department of Agronomy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, ChinaZhejiang Key Laboratory of Crop Germplasm Resource, Department of Agronomy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, ChinaZhejiang Key Laboratory of Crop Germplasm Resource, Department of Agronomy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, ChinaDow International Medical College, Dow University of Health Sciences, Karachi, PakistanZhejiang Key Laboratory of Crop Germplasm Resource, Department of Agronomy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, ChinaFaculty of Agriculture, Lasbela University of Agriculture, Water and Marine Sciences, Uthal, PakistanFaculty of Agriculture, Lasbela University of Agriculture, Water and Marine Sciences, Uthal, PakistanFaculty of Science, Universiti Brunei Darussalam, Bandar Seri Begawan, BruneiChina National Rice Research Institute (CNRRI), Fuyang, ChinaGuizhou Academy of Tobacco Science, Guizhou, ChinaZhejiang Key Laboratory of Crop Germplasm Resource, Department of Agronomy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, ChinaIn the context of eco-sustainable acquisition of food security, arsenic (As) acts as a deterring factor, which easily infiltrates our food chain via plant uptake. Therefore, devising climate-smart strategies becomes exigent for minimizing the imposed risks. Pertinently, Serendipita indica (S. indica) is well reputed for its post-symbiotic stress alleviatory and phyto-promotive potential. Management of phosphorus (P) is acclaimed for mitigating arsenic toxicity in plants by inhibiting the uptake of As molecules due to the competitive cationic exchange in the rhizosphere. The current study was designed to investigate the tandem effects of S. indica and P in combating As toxicity employing two rice genotypes, i.e., Guodao-6 (GD-6; As-sensitive genotype) and Zhongzhe You-1 (ZZY-1; As-tolerant genotype). After successful fungal colonization, alone and combined arsenic (10 μ M L−1) and phosphorus (50 μ M L−1) treatments were applied. Results displayed that the recuperating effects of combined S. indica and P treatment were indeed much profound than their alone treatments; however, most of the beneficial influences were harnessed by ZZY-1 in comparison with GD-6. Distinct genotypic differences were observed for antioxidant enzyme activities, which were induced slightly higher in S. indica-colonized ZZY-1 plants, with or without additional P, as compared to GD-6. Ultrastructure images of root and shoot exhibited ravages of As in the form of chloroplasts-, nuclei-and cell wall-damage with enlarged vacuole area, mellowed mostly by the combined treatment of S. indica and P in both genotypes. Gene expression of PHTs family transporters was regulated at different levels in almost all treatments across genotypes. Conclusively, the results of this study validated the promising role of S. indica and additional P in mitigating As stress, albeit corroborated that the extent of relevant benefit exploitation is highly genotype-dependent. Verily, unlocking the potential of nature-friendly solutions will mend the anthropogenic damage already been done to our environment.https://www.frontiersin.org/articles/10.3389/fpls.2022.982668/fullarsenic toxicityendophytic fungusphosphorous nutritionOryza sativaantioxidant enzymesgene expression analysis |
| spellingShingle | Shafaque Sehar Qidong Feng Muhammad Faheem Adil Falak Sehar Sahito Zakir Ibrahim Zakir Ibrahim Dost Muhammad Baloch Najeeb Ullah Younan Ouyang Yushuang Guo Imran Haider Shamsi Tandem application of endophytic fungus Serendipita indica and phosphorus synergistically recuperate arsenic induced stress in rice Frontiers in Plant Science arsenic toxicity endophytic fungus phosphorous nutrition Oryza sativa antioxidant enzymes gene expression analysis |
| title | Tandem application of endophytic fungus Serendipita indica and phosphorus synergistically recuperate arsenic induced stress in rice |
| title_full | Tandem application of endophytic fungus Serendipita indica and phosphorus synergistically recuperate arsenic induced stress in rice |
| title_fullStr | Tandem application of endophytic fungus Serendipita indica and phosphorus synergistically recuperate arsenic induced stress in rice |
| title_full_unstemmed | Tandem application of endophytic fungus Serendipita indica and phosphorus synergistically recuperate arsenic induced stress in rice |
| title_short | Tandem application of endophytic fungus Serendipita indica and phosphorus synergistically recuperate arsenic induced stress in rice |
| title_sort | tandem application of endophytic fungus serendipita indica and phosphorus synergistically recuperate arsenic induced stress in rice |
| topic | arsenic toxicity endophytic fungus phosphorous nutrition Oryza sativa antioxidant enzymes gene expression analysis |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.982668/full |
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