Comparative Physiological and Transcriptomic Analyses of Improved Heat Stress Tolerance in Celery (<i>Apium Graveolens</i> L.) Caused by Exogenous Melatonin
Melatonin (MT) is crucial in plant growth, development, and response to stress. Celery is a vegetable that grows in a cool climate, and a hot climate can deteriorate its growth, yield, and quality. This study investigates the effect of exogenous melatonin on celery physiology. Transcriptional levels...
| Main Authors: | , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-09-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/23/19/11382 |
| _version_ | 1797479079441596416 |
|---|---|
| author | Mengyao Li Jin Zhou Jiageng Du Xiaoyan Li Yue Sun Zhuo Wang Yuanxiu Lin Yunting Zhang Yan Wang Wen He Xiaorong Wang Qing Chen Yong Zhang Ya Luo Haoru Tang |
| author_facet | Mengyao Li Jin Zhou Jiageng Du Xiaoyan Li Yue Sun Zhuo Wang Yuanxiu Lin Yunting Zhang Yan Wang Wen He Xiaorong Wang Qing Chen Yong Zhang Ya Luo Haoru Tang |
| author_sort | Mengyao Li |
| collection | DOAJ |
| description | Melatonin (MT) is crucial in plant growth, development, and response to stress. Celery is a vegetable that grows in a cool climate, and a hot climate can deteriorate its growth, yield, and quality. This study investigates the effect of exogenous melatonin on celery physiology. Transcriptional levels were analyzed by spraying celery with exogenous MT before exposing it to high temperatures. The regulatory mechanism of exogenous MT-mediated heat tolerance was examined. The results show that the exogenous MT reduced the thermal damage state of celery seedlings, as well as the malondialdehyde (MDA) content and relative conductivity (REC), increasing the oxidase activity, the osmotic regulatory substances, and chlorophyll, enhancing the leaf transpiration and the light energy utilization efficiency. We examined the mechanism of exogenous MT in mitigating high-temperature damage using the transcriptome sequencing method. A total of 134 genes were expressed differently at high temperature in the celery treated with MT compared with the untreated celery. Functional annotation analysis revealed that the differentially expressed genes were abundant in the “pyruvate metabolism” pathway and the “peroxidase activity” pathway. According to the pathway-based gene expression analysis, exogenous MT can inhibit the upregulation of pyruvate synthesis genes and the downregulation of pyruvate consumption genes, preventing the accumulated pyruvate from rapidly upregulating the expression of peroxidase genes, and thereby enhancing peroxidase activity. RT-qPCR verification showed a rising encoding peroxidase gene expression under MT treatment. The gene expression pattern involved in pyruvate anabolism and metabolism agreed with the abundant transcriptome expression, validating the physiological index results. These results indicate that the application of exogenous MT to celery significantly enhances the ability of plant to remove reactive oxygen species (ROS) in response to heat stress, thereby improving the ability of plant to resist heat stress. The results of this study provide a theoretical basis for the use of MT to alleviate the damage caused by heat stress in plant growth and development. |
| first_indexed | 2024-03-09T21:40:42Z |
| format | Article |
| id | doaj.art-eaec02b4d88e49b395f3f253540b0536 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T21:40:42Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-eaec02b4d88e49b395f3f253540b05362023-11-23T20:32:11ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-09-0123191138210.3390/ijms231911382Comparative Physiological and Transcriptomic Analyses of Improved Heat Stress Tolerance in Celery (<i>Apium Graveolens</i> L.) Caused by Exogenous MelatoninMengyao Li0Jin Zhou1Jiageng Du2Xiaoyan Li3Yue Sun4Zhuo Wang5Yuanxiu Lin6Yunting Zhang7Yan Wang8Wen He9Xiaorong Wang10Qing Chen11Yong Zhang12Ya Luo13Haoru Tang14College of Horticulture, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu 611130, ChinaMelatonin (MT) is crucial in plant growth, development, and response to stress. Celery is a vegetable that grows in a cool climate, and a hot climate can deteriorate its growth, yield, and quality. This study investigates the effect of exogenous melatonin on celery physiology. Transcriptional levels were analyzed by spraying celery with exogenous MT before exposing it to high temperatures. The regulatory mechanism of exogenous MT-mediated heat tolerance was examined. The results show that the exogenous MT reduced the thermal damage state of celery seedlings, as well as the malondialdehyde (MDA) content and relative conductivity (REC), increasing the oxidase activity, the osmotic regulatory substances, and chlorophyll, enhancing the leaf transpiration and the light energy utilization efficiency. We examined the mechanism of exogenous MT in mitigating high-temperature damage using the transcriptome sequencing method. A total of 134 genes were expressed differently at high temperature in the celery treated with MT compared with the untreated celery. Functional annotation analysis revealed that the differentially expressed genes were abundant in the “pyruvate metabolism” pathway and the “peroxidase activity” pathway. According to the pathway-based gene expression analysis, exogenous MT can inhibit the upregulation of pyruvate synthesis genes and the downregulation of pyruvate consumption genes, preventing the accumulated pyruvate from rapidly upregulating the expression of peroxidase genes, and thereby enhancing peroxidase activity. RT-qPCR verification showed a rising encoding peroxidase gene expression under MT treatment. The gene expression pattern involved in pyruvate anabolism and metabolism agreed with the abundant transcriptome expression, validating the physiological index results. These results indicate that the application of exogenous MT to celery significantly enhances the ability of plant to remove reactive oxygen species (ROS) in response to heat stress, thereby improving the ability of plant to resist heat stress. The results of this study provide a theoretical basis for the use of MT to alleviate the damage caused by heat stress in plant growth and development.https://www.mdpi.com/1422-0067/23/19/11382antioxidant capacity<i>Apium graveolens</i> L.exogenous melatoninheat stresstranscriptomic analyses |
| spellingShingle | Mengyao Li Jin Zhou Jiageng Du Xiaoyan Li Yue Sun Zhuo Wang Yuanxiu Lin Yunting Zhang Yan Wang Wen He Xiaorong Wang Qing Chen Yong Zhang Ya Luo Haoru Tang Comparative Physiological and Transcriptomic Analyses of Improved Heat Stress Tolerance in Celery (<i>Apium Graveolens</i> L.) Caused by Exogenous Melatonin International Journal of Molecular Sciences antioxidant capacity <i>Apium graveolens</i> L. exogenous melatonin heat stress transcriptomic analyses |
| title | Comparative Physiological and Transcriptomic Analyses of Improved Heat Stress Tolerance in Celery (<i>Apium Graveolens</i> L.) Caused by Exogenous Melatonin |
| title_full | Comparative Physiological and Transcriptomic Analyses of Improved Heat Stress Tolerance in Celery (<i>Apium Graveolens</i> L.) Caused by Exogenous Melatonin |
| title_fullStr | Comparative Physiological and Transcriptomic Analyses of Improved Heat Stress Tolerance in Celery (<i>Apium Graveolens</i> L.) Caused by Exogenous Melatonin |
| title_full_unstemmed | Comparative Physiological and Transcriptomic Analyses of Improved Heat Stress Tolerance in Celery (<i>Apium Graveolens</i> L.) Caused by Exogenous Melatonin |
| title_short | Comparative Physiological and Transcriptomic Analyses of Improved Heat Stress Tolerance in Celery (<i>Apium Graveolens</i> L.) Caused by Exogenous Melatonin |
| title_sort | comparative physiological and transcriptomic analyses of improved heat stress tolerance in celery i apium graveolens i l caused by exogenous melatonin |
| topic | antioxidant capacity <i>Apium graveolens</i> L. exogenous melatonin heat stress transcriptomic analyses |
| url | https://www.mdpi.com/1422-0067/23/19/11382 |
| work_keys_str_mv | AT mengyaoli comparativephysiologicalandtranscriptomicanalysesofimprovedheatstresstoleranceinceleryiapiumgraveolensilcausedbyexogenousmelatonin AT jinzhou comparativephysiologicalandtranscriptomicanalysesofimprovedheatstresstoleranceinceleryiapiumgraveolensilcausedbyexogenousmelatonin AT jiagengdu comparativephysiologicalandtranscriptomicanalysesofimprovedheatstresstoleranceinceleryiapiumgraveolensilcausedbyexogenousmelatonin AT xiaoyanli comparativephysiologicalandtranscriptomicanalysesofimprovedheatstresstoleranceinceleryiapiumgraveolensilcausedbyexogenousmelatonin AT yuesun comparativephysiologicalandtranscriptomicanalysesofimprovedheatstresstoleranceinceleryiapiumgraveolensilcausedbyexogenousmelatonin AT zhuowang comparativephysiologicalandtranscriptomicanalysesofimprovedheatstresstoleranceinceleryiapiumgraveolensilcausedbyexogenousmelatonin AT yuanxiulin comparativephysiologicalandtranscriptomicanalysesofimprovedheatstresstoleranceinceleryiapiumgraveolensilcausedbyexogenousmelatonin AT yuntingzhang comparativephysiologicalandtranscriptomicanalysesofimprovedheatstresstoleranceinceleryiapiumgraveolensilcausedbyexogenousmelatonin AT yanwang comparativephysiologicalandtranscriptomicanalysesofimprovedheatstresstoleranceinceleryiapiumgraveolensilcausedbyexogenousmelatonin AT wenhe comparativephysiologicalandtranscriptomicanalysesofimprovedheatstresstoleranceinceleryiapiumgraveolensilcausedbyexogenousmelatonin AT xiaorongwang comparativephysiologicalandtranscriptomicanalysesofimprovedheatstresstoleranceinceleryiapiumgraveolensilcausedbyexogenousmelatonin AT qingchen comparativephysiologicalandtranscriptomicanalysesofimprovedheatstresstoleranceinceleryiapiumgraveolensilcausedbyexogenousmelatonin AT yongzhang comparativephysiologicalandtranscriptomicanalysesofimprovedheatstresstoleranceinceleryiapiumgraveolensilcausedbyexogenousmelatonin AT yaluo comparativephysiologicalandtranscriptomicanalysesofimprovedheatstresstoleranceinceleryiapiumgraveolensilcausedbyexogenousmelatonin AT haorutang comparativephysiologicalandtranscriptomicanalysesofimprovedheatstresstoleranceinceleryiapiumgraveolensilcausedbyexogenousmelatonin |