Absence of Depressive and Anxious Behavior with Genetic Dysregulation in Adult C57Bl/6J Mice after Prenatal Exposure to Ionizing Radiation
The exposure of ionizing radiation during early gestation often leads to deleterious and even lethal effects; however, few extensive studies have been conducted on late gestational exposures. This research examined the behavior al effects of C57Bl/6J mouse offspring exposed to low dose ionizing gamm...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
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MDPI AG
2023-05-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/10/8466 |
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| author | Christine Lalonde Shayenthiran Sreetharan Alyssa Murray Lisa Stoa Mary Ellen Cybulski Allison Kennedy Nicholas Landry Amy Stillar Sandhya Khurana Sujeenthar Tharmalingam Joanna Wilson Neelam Khaper Simon J. Lees Douglas Boreham T. C. Tai |
| author_facet | Christine Lalonde Shayenthiran Sreetharan Alyssa Murray Lisa Stoa Mary Ellen Cybulski Allison Kennedy Nicholas Landry Amy Stillar Sandhya Khurana Sujeenthar Tharmalingam Joanna Wilson Neelam Khaper Simon J. Lees Douglas Boreham T. C. Tai |
| author_sort | Christine Lalonde |
| collection | DOAJ |
| description | The exposure of ionizing radiation during early gestation often leads to deleterious and even lethal effects; however, few extensive studies have been conducted on late gestational exposures. This research examined the behavior al effects of C57Bl/6J mouse offspring exposed to low dose ionizing gamma irradiation during the equivalent third trimester. Pregnant dams were randomly assigned to sham or exposed groups to either low dose or sublethal dose radiation (50, 300, or 1000 mGy) at gestational day 15. Adult offspring underwent a behavioral and genetic analysis after being raised under normal murine housing conditions. Our results indicate very little change in the behavioral tasks measuring general anxiety, social anxiety, and stress-management in animals exposed prenatally across the low dose radiation conditions. Quantitative real-time polymerase chain reactions were conducted on the cerebral cortex, hippocampus, and cerebellum of each animal; results indicate some dysregulation in markers of DNA damage, synaptic activity, reactive oxygen species (ROS) regulation, and methylation pathways in the offspring. Together, our results provide evidence in the C57Bl/6J strain, that exposure to sublethal dose radiation (<1000 mGy) during the last period of gestation leads to no observable changes in behaviour when assessed as adults, although some changes in gene expression were observed for specific brain regions. These results indicate that the level of oxidative stress occurring during late gestation for this mouse strain is not sufficient for a change in the assessed behavioral phenotype, but results in some modest dysregulation of the genetic profile of the brain. |
| first_indexed | 2024-03-11T03:41:02Z |
| format | Article |
| id | doaj.art-eaf24def52d14937a04f4f643172e127 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T03:41:02Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-eaf24def52d14937a04f4f643172e1272023-11-18T01:36:16ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-05-012410846610.3390/ijms24108466Absence of Depressive and Anxious Behavior with Genetic Dysregulation in Adult C57Bl/6J Mice after Prenatal Exposure to Ionizing RadiationChristine Lalonde0Shayenthiran Sreetharan1Alyssa Murray2Lisa Stoa3Mary Ellen Cybulski4Allison Kennedy5Nicholas Landry6Amy Stillar7Sandhya Khurana8Sujeenthar Tharmalingam9Joanna Wilson10Neelam Khaper11Simon J. Lees12Douglas Boreham13T. C. Tai14Biomolecular Sciences, Laurentian University, Sudbury, ON P3E2C6, CanadaMedical Sciences Division, NOSM University, Sudbury, ON P3E2C6, CanadaSchool of Natural Sciences, Laurentian University, Sudbury, ON P3E2C6, CanadaDepartment of Biology, McMaster University, Hamilton, ON L8S4L8, CanadaMedical Sciences Division, NOSM University, Sudbury, ON P3E2C6, CanadaMedical Sciences Division, NOSM University, Sudbury, ON P3E2C6, CanadaDepartment of Psychology, Nipissing University, North Bay, ON P1B8L7, CanadaDepartment of Psychology, Nipissing University, North Bay, ON P1B8L7, CanadaMedical Sciences Division, NOSM University, Sudbury, ON P3E2C6, CanadaBiomolecular Sciences, Laurentian University, Sudbury, ON P3E2C6, CanadaDepartment of Biology, McMaster University, Hamilton, ON L8S4L8, CanadaBiomolecular Sciences, Laurentian University, Sudbury, ON P3E2C6, CanadaMedical Sciences Division, NOSM University, Sudbury, ON P3E2C6, CanadaBiomolecular Sciences, Laurentian University, Sudbury, ON P3E2C6, CanadaBiomolecular Sciences, Laurentian University, Sudbury, ON P3E2C6, CanadaThe exposure of ionizing radiation during early gestation often leads to deleterious and even lethal effects; however, few extensive studies have been conducted on late gestational exposures. This research examined the behavior al effects of C57Bl/6J mouse offspring exposed to low dose ionizing gamma irradiation during the equivalent third trimester. Pregnant dams were randomly assigned to sham or exposed groups to either low dose or sublethal dose radiation (50, 300, or 1000 mGy) at gestational day 15. Adult offspring underwent a behavioral and genetic analysis after being raised under normal murine housing conditions. Our results indicate very little change in the behavioral tasks measuring general anxiety, social anxiety, and stress-management in animals exposed prenatally across the low dose radiation conditions. Quantitative real-time polymerase chain reactions were conducted on the cerebral cortex, hippocampus, and cerebellum of each animal; results indicate some dysregulation in markers of DNA damage, synaptic activity, reactive oxygen species (ROS) regulation, and methylation pathways in the offspring. Together, our results provide evidence in the C57Bl/6J strain, that exposure to sublethal dose radiation (<1000 mGy) during the last period of gestation leads to no observable changes in behaviour when assessed as adults, although some changes in gene expression were observed for specific brain regions. These results indicate that the level of oxidative stress occurring during late gestation for this mouse strain is not sufficient for a change in the assessed behavioral phenotype, but results in some modest dysregulation of the genetic profile of the brain.https://www.mdpi.com/1422-0067/24/10/8466radiationfetal programminglate gestationdepressionanxiety |
| spellingShingle | Christine Lalonde Shayenthiran Sreetharan Alyssa Murray Lisa Stoa Mary Ellen Cybulski Allison Kennedy Nicholas Landry Amy Stillar Sandhya Khurana Sujeenthar Tharmalingam Joanna Wilson Neelam Khaper Simon J. Lees Douglas Boreham T. C. Tai Absence of Depressive and Anxious Behavior with Genetic Dysregulation in Adult C57Bl/6J Mice after Prenatal Exposure to Ionizing Radiation International Journal of Molecular Sciences radiation fetal programming late gestation depression anxiety |
| title | Absence of Depressive and Anxious Behavior with Genetic Dysregulation in Adult C57Bl/6J Mice after Prenatal Exposure to Ionizing Radiation |
| title_full | Absence of Depressive and Anxious Behavior with Genetic Dysregulation in Adult C57Bl/6J Mice after Prenatal Exposure to Ionizing Radiation |
| title_fullStr | Absence of Depressive and Anxious Behavior with Genetic Dysregulation in Adult C57Bl/6J Mice after Prenatal Exposure to Ionizing Radiation |
| title_full_unstemmed | Absence of Depressive and Anxious Behavior with Genetic Dysregulation in Adult C57Bl/6J Mice after Prenatal Exposure to Ionizing Radiation |
| title_short | Absence of Depressive and Anxious Behavior with Genetic Dysregulation in Adult C57Bl/6J Mice after Prenatal Exposure to Ionizing Radiation |
| title_sort | absence of depressive and anxious behavior with genetic dysregulation in adult c57bl 6j mice after prenatal exposure to ionizing radiation |
| topic | radiation fetal programming late gestation depression anxiety |
| url | https://www.mdpi.com/1422-0067/24/10/8466 |
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