A Multi-Omics Study of Human Testis and Epididymis
The human testis and epididymis play critical roles in male fertility, including the spermatogenesis process, sperm storage, and maturation. However, the unique functions of the two organs had not been systematically studied. Herein, we provide a systematic and comprehensive multi-omics study betwee...
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MDPI AG
2021-06-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/11/3345 |
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| author | Weimin Zheng Yang Zhang Chuanyu Sun Shengyang Ge Yifan Tan Huali Shen Pengyuan Yang |
| author_facet | Weimin Zheng Yang Zhang Chuanyu Sun Shengyang Ge Yifan Tan Huali Shen Pengyuan Yang |
| author_sort | Weimin Zheng |
| collection | DOAJ |
| description | The human testis and epididymis play critical roles in male fertility, including the spermatogenesis process, sperm storage, and maturation. However, the unique functions of the two organs had not been systematically studied. Herein, we provide a systematic and comprehensive multi-omics study between testis and epididymis. RNA-Seq profiling detected and quantified 19,653 in the testis and 18,407 in the epididymis. Proteomic profiling resulted in the identification of a total of 11,024 and 10,386 proteins in the testis and epididymis, respectively, including 110 proteins that previously have been classified as MPs (missing proteins). Furthermore, Five MPs expressed in testis were validated by the MRM method. Subsequently, multi-omcis between testis and epididymis were performed, including biological functions and pathways of DEGs (Differentially Expressed Genes) in each group, revealing that those differences were related to spermatogenesis, male gamete generation, as well as reproduction. In conclusion, this study can help us find the expression regularity of missing protein and help related scientists understand the physiological functions of testis and epididymis more deeply. |
| first_indexed | 2024-03-10T10:47:18Z |
| format | Article |
| id | doaj.art-eafa8dfd1eb64d41aad20439499e16c0 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T10:47:18Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-eafa8dfd1eb64d41aad20439499e16c02023-11-21T22:30:44ZengMDPI AGMolecules1420-30492021-06-012611334510.3390/molecules26113345A Multi-Omics Study of Human Testis and EpididymisWeimin Zheng0Yang Zhang1Chuanyu Sun2Shengyang Ge3Yifan Tan4Huali Shen5Pengyuan Yang6Department of Chemistry, Fudan University, Shanghai 200433, ChinaDepartment of Systems Biology for Medicine and Institutes of Biomedical Sciences, Shanghai Medical College, Fudan University, Shanghai 200032, ChinaDepartment of Urology, Huashan Hospital, Fudan University, Shanghai 200040, ChinaDepartment of Urology, Huashan Hospital, Fudan University, Shanghai 200040, ChinaDepartment of Urology, Huashan Hospital, Fudan University, Shanghai 200040, ChinaDepartment of Systems Biology for Medicine and Institutes of Biomedical Sciences, Shanghai Medical College, Fudan University, Shanghai 200032, ChinaDepartment of Chemistry, Fudan University, Shanghai 200433, ChinaThe human testis and epididymis play critical roles in male fertility, including the spermatogenesis process, sperm storage, and maturation. However, the unique functions of the two organs had not been systematically studied. Herein, we provide a systematic and comprehensive multi-omics study between testis and epididymis. RNA-Seq profiling detected and quantified 19,653 in the testis and 18,407 in the epididymis. Proteomic profiling resulted in the identification of a total of 11,024 and 10,386 proteins in the testis and epididymis, respectively, including 110 proteins that previously have been classified as MPs (missing proteins). Furthermore, Five MPs expressed in testis were validated by the MRM method. Subsequently, multi-omcis between testis and epididymis were performed, including biological functions and pathways of DEGs (Differentially Expressed Genes) in each group, revealing that those differences were related to spermatogenesis, male gamete generation, as well as reproduction. In conclusion, this study can help us find the expression regularity of missing protein and help related scientists understand the physiological functions of testis and epididymis more deeply.https://www.mdpi.com/1420-3049/26/11/3345proteomicstranscriptomemissing proteinbeta-defensinMRM technique |
| spellingShingle | Weimin Zheng Yang Zhang Chuanyu Sun Shengyang Ge Yifan Tan Huali Shen Pengyuan Yang A Multi-Omics Study of Human Testis and Epididymis Molecules proteomics transcriptome missing protein beta-defensin MRM technique |
| title | A Multi-Omics Study of Human Testis and Epididymis |
| title_full | A Multi-Omics Study of Human Testis and Epididymis |
| title_fullStr | A Multi-Omics Study of Human Testis and Epididymis |
| title_full_unstemmed | A Multi-Omics Study of Human Testis and Epididymis |
| title_short | A Multi-Omics Study of Human Testis and Epididymis |
| title_sort | multi omics study of human testis and epididymis |
| topic | proteomics transcriptome missing protein beta-defensin MRM technique |
| url | https://www.mdpi.com/1420-3049/26/11/3345 |
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