Codon Usage and Adenovirus Fitness: Implications for Vaccine Development
Vaccination is the most effective method to date to prevent viral diseases. It intends to mimic a naturally occurring infection while avoiding the disease, exposing our bodies to viral antigens to trigger an immune response that will protect us from future infections. Among different strategies for...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-02-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.633946/full |
| _version_ | 1818916391547305984 |
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| author | Judit Giménez-Roig Estela Núñez-Manchón Ramon Alemany Eneko Villanueva Cristina Fillat Cristina Fillat Cristina Fillat |
| author_facet | Judit Giménez-Roig Estela Núñez-Manchón Ramon Alemany Eneko Villanueva Cristina Fillat Cristina Fillat Cristina Fillat |
| author_sort | Judit Giménez-Roig |
| collection | DOAJ |
| description | Vaccination is the most effective method to date to prevent viral diseases. It intends to mimic a naturally occurring infection while avoiding the disease, exposing our bodies to viral antigens to trigger an immune response that will protect us from future infections. Among different strategies for vaccine development, recombinant vaccines are one of the most efficient ones. Recombinant vaccines use safe viral vectors as vehicles and incorporate a transgenic antigen of the pathogen against which we intend to generate an immune response. These vaccines can be based on replication-deficient viruses or replication-competent viruses. While the most effective strategy involves replication-competent viruses, they must be attenuated to prevent any health hazard while guaranteeing a strong humoral and cellular immune response. Several attenuation strategies for adenoviral-based vaccine development have been contemplated over time. In this paper, we will review them and discuss novel approaches based on the principle that protein synthesis from individual genes can be modulated by codon usage bias manipulation. We will summarize vaccine approaches that consider recoding of viral proteins to produce adenoviral attenuation and recoding of the transgene antigens for both viral attenuation and efficient viral epitope expression. |
| first_indexed | 2024-12-20T00:17:26Z |
| format | Article |
| id | doaj.art-eafe543a960a4808b6765f4110b58474 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-20T00:17:26Z |
| publishDate | 2021-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-eafe543a960a4808b6765f4110b584742022-12-21T20:00:17ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-02-011210.3389/fmicb.2021.633946633946Codon Usage and Adenovirus Fitness: Implications for Vaccine DevelopmentJudit Giménez-Roig0Estela Núñez-Manchón1Ramon Alemany2Eneko Villanueva3Cristina Fillat4Cristina Fillat5Cristina Fillat6Institut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, SpainInstitut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, SpainProcure Program, Institut Català d’Oncologia- Oncobell Program, IDIBELL, L’Hospitalet de Llobregat, Barcelona, SpainCambridge Centre for Proteomics, Department of Biochemistry, University of Cambridge, Cambridge, United KingdomInstitut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, SpainCentro de Investigación Biomédica en Red de Enfermedades Raras (CIBERER), Barcelona, SpainFacultat de Medicina i Ciències de la Salut, Universitat de Barcelona (UB), Barcelona, SpainVaccination is the most effective method to date to prevent viral diseases. It intends to mimic a naturally occurring infection while avoiding the disease, exposing our bodies to viral antigens to trigger an immune response that will protect us from future infections. Among different strategies for vaccine development, recombinant vaccines are one of the most efficient ones. Recombinant vaccines use safe viral vectors as vehicles and incorporate a transgenic antigen of the pathogen against which we intend to generate an immune response. These vaccines can be based on replication-deficient viruses or replication-competent viruses. While the most effective strategy involves replication-competent viruses, they must be attenuated to prevent any health hazard while guaranteeing a strong humoral and cellular immune response. Several attenuation strategies for adenoviral-based vaccine development have been contemplated over time. In this paper, we will review them and discuss novel approaches based on the principle that protein synthesis from individual genes can be modulated by codon usage bias manipulation. We will summarize vaccine approaches that consider recoding of viral proteins to produce adenoviral attenuation and recoding of the transgene antigens for both viral attenuation and efficient viral epitope expression.https://www.frontiersin.org/articles/10.3389/fmicb.2021.633946/fullcodon optimizationviral attenuationlive-attenuated vaccinesadenovirus-based vaccinescodon usage bias |
| spellingShingle | Judit Giménez-Roig Estela Núñez-Manchón Ramon Alemany Eneko Villanueva Cristina Fillat Cristina Fillat Cristina Fillat Codon Usage and Adenovirus Fitness: Implications for Vaccine Development Frontiers in Microbiology codon optimization viral attenuation live-attenuated vaccines adenovirus-based vaccines codon usage bias |
| title | Codon Usage and Adenovirus Fitness: Implications for Vaccine Development |
| title_full | Codon Usage and Adenovirus Fitness: Implications for Vaccine Development |
| title_fullStr | Codon Usage and Adenovirus Fitness: Implications for Vaccine Development |
| title_full_unstemmed | Codon Usage and Adenovirus Fitness: Implications for Vaccine Development |
| title_short | Codon Usage and Adenovirus Fitness: Implications for Vaccine Development |
| title_sort | codon usage and adenovirus fitness implications for vaccine development |
| topic | codon optimization viral attenuation live-attenuated vaccines adenovirus-based vaccines codon usage bias |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.633946/full |
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