Effects of high-risk human papillomavirus infection on P53, pRb, and survivin in lung adenocarcinoma—a retrospective study
Objective To observe the effects of high-risk human papillomavirus (HR-HPV) infection on P53, pRb, and survivin in lung adenocarcinoma (LUAD). Methods The cancerous and cancer-adjacent tissues of 102 patients with LUAD from January 2020 to April 2022 were selected for the study. HR-HPV infection was...
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PeerJ Inc.
2023-07-01
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| Online Access: | https://peerj.com/articles/15570.pdf |
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| author | Wenwen Sun Hui Yang Lu Cao Ruochen Wu Baoqi Ding Xiaocui Liu Xinli Wang Qiang Zhang |
| author_facet | Wenwen Sun Hui Yang Lu Cao Ruochen Wu Baoqi Ding Xiaocui Liu Xinli Wang Qiang Zhang |
| author_sort | Wenwen Sun |
| collection | DOAJ |
| description | Objective To observe the effects of high-risk human papillomavirus (HR-HPV) infection on P53, pRb, and survivin in lung adenocarcinoma (LUAD). Methods The cancerous and cancer-adjacent tissues of 102 patients with LUAD from January 2020 to April 2022 were selected for the study. HR-HPV infection was detected by flow fluorescence method, and P53, pRb, and survivin protein expression was detected by immunohistochemical staining method. Statistical analysis was performed to determine the differences in the HR-HPV infection and the expression of P53, pRb, and survivin proteins between LUAD tissues and cancer-adjacent tissues; the correlation between HR-HPV infection and P53, pRb, and survivin protein expression in cancer tissues; and the correlation between HR-HPV infection and clinicopathological features of LUAD. Results The infection rate of HR-HPV was higher in the LUAD tissues (28.43%) than in cancer-adjacent tissues (7.84%), and the difference was statistically significant (P < 0.05). The positive rates of P53 and survivin protein were higher in the LUAD group (33.33% and 67.16%, respectively) than in the cancer-adjacent group (3.92% and 11.73%, respectively), and the difference was statistically significant (P < 0.05). The positive rate of pRb protein was lower in the LUAD group (58.82%) than in the cancer-adjacent group (92.14%), and the difference was statistically significant (P < 0.05). The positive rates of P53 and survivin proteins were significantly higher in the HR-HPV LUAD group (58.62% and 86.21%, respectively) than in the non-HR-HPV LUAD group (41.38% and 67.12%, respectively), and the difference was statistically significant (P < 0.05). The expression rate of pRb protein was significantly lower in the HR-HPV LUAD group (37.93%) than in the non-HR-HPV LUAD group (67.12%), and the difference was statistically significant (P < 0.05). The expression of p53 and survivin protein was positively correlated with HR-HPV infection (r = 0.338 and 0.444, P < 0.05), whereas the expression of pRb protein was negatively correlated with HR-HPV infection (r = − 0.268, P < 0.05). HR-HPV infection was not associated with gender, age, and smoking in patients with LUAD (P > 0.05). HR-HPV infection was associated with lymph node metastasis and clinical stage of LUAD (P < 0.05). Conclusions HR-HPV infection was associated with lymph node metastasis and clinical stage of LUAD, which may be achieved by up-regulating p53 and survivin protein expression and down-regulating pRb protein expression. |
| first_indexed | 2024-03-09T08:04:57Z |
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| language | English |
| last_indexed | 2024-03-09T08:04:57Z |
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| spelling | doaj.art-eafe7b06a95c475abdcb40182a4066bd2023-12-03T00:24:19ZengPeerJ Inc.PeerJ2167-83592023-07-0111e1557010.7717/peerj.15570Effects of high-risk human papillomavirus infection on P53, pRb, and survivin in lung adenocarcinoma—a retrospective studyWenwen Sun0Hui Yang1Lu Cao2Ruochen Wu3Baoqi Ding4Xiaocui Liu5Xinli Wang6Qiang Zhang7Department of Pathology, The Second Affiliated Hospital of Shandong First Medical University, Taian, Shandong, ChinaDepartment of Pathology, The Second Affiliated Hospital of Shandong First Medical University, Taian, Shandong, ChinaDepartment of Pathology, The Second Affiliated Hospital of Shandong First Medical University, Taian, Shandong, ChinaDepartment of Pathology, The Second Affiliated Hospital of Shandong First Medical University, Taian, Shandong, ChinaDepartment of Pathology, The Second Affiliated Hospital of Shandong First Medical University, Taian, Shandong, ChinaDepartment of Histoloembryology, School of Clinical and Basic Medicine, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan, Shandong, ChinaDepartment of Pathology, The Second Affiliated Hospital of Shandong First Medical University, Taian, Shandong, ChinaShandong First Medical University, Jinan, Shandong, TaianObjective To observe the effects of high-risk human papillomavirus (HR-HPV) infection on P53, pRb, and survivin in lung adenocarcinoma (LUAD). Methods The cancerous and cancer-adjacent tissues of 102 patients with LUAD from January 2020 to April 2022 were selected for the study. HR-HPV infection was detected by flow fluorescence method, and P53, pRb, and survivin protein expression was detected by immunohistochemical staining method. Statistical analysis was performed to determine the differences in the HR-HPV infection and the expression of P53, pRb, and survivin proteins between LUAD tissues and cancer-adjacent tissues; the correlation between HR-HPV infection and P53, pRb, and survivin protein expression in cancer tissues; and the correlation between HR-HPV infection and clinicopathological features of LUAD. Results The infection rate of HR-HPV was higher in the LUAD tissues (28.43%) than in cancer-adjacent tissues (7.84%), and the difference was statistically significant (P < 0.05). The positive rates of P53 and survivin protein were higher in the LUAD group (33.33% and 67.16%, respectively) than in the cancer-adjacent group (3.92% and 11.73%, respectively), and the difference was statistically significant (P < 0.05). The positive rate of pRb protein was lower in the LUAD group (58.82%) than in the cancer-adjacent group (92.14%), and the difference was statistically significant (P < 0.05). The positive rates of P53 and survivin proteins were significantly higher in the HR-HPV LUAD group (58.62% and 86.21%, respectively) than in the non-HR-HPV LUAD group (41.38% and 67.12%, respectively), and the difference was statistically significant (P < 0.05). The expression rate of pRb protein was significantly lower in the HR-HPV LUAD group (37.93%) than in the non-HR-HPV LUAD group (67.12%), and the difference was statistically significant (P < 0.05). The expression of p53 and survivin protein was positively correlated with HR-HPV infection (r = 0.338 and 0.444, P < 0.05), whereas the expression of pRb protein was negatively correlated with HR-HPV infection (r = − 0.268, P < 0.05). HR-HPV infection was not associated with gender, age, and smoking in patients with LUAD (P > 0.05). HR-HPV infection was associated with lymph node metastasis and clinical stage of LUAD (P < 0.05). Conclusions HR-HPV infection was associated with lymph node metastasis and clinical stage of LUAD, which may be achieved by up-regulating p53 and survivin protein expression and down-regulating pRb protein expression.https://peerj.com/articles/15570.pdfHuman papillomavirusLung adenocarcinomaP53PRbSurvivinSmoking |
| spellingShingle | Wenwen Sun Hui Yang Lu Cao Ruochen Wu Baoqi Ding Xiaocui Liu Xinli Wang Qiang Zhang Effects of high-risk human papillomavirus infection on P53, pRb, and survivin in lung adenocarcinoma—a retrospective study PeerJ Human papillomavirus Lung adenocarcinoma P53 PRb Survivin Smoking |
| title | Effects of high-risk human papillomavirus infection on P53, pRb, and survivin in lung adenocarcinoma—a retrospective study |
| title_full | Effects of high-risk human papillomavirus infection on P53, pRb, and survivin in lung adenocarcinoma—a retrospective study |
| title_fullStr | Effects of high-risk human papillomavirus infection on P53, pRb, and survivin in lung adenocarcinoma—a retrospective study |
| title_full_unstemmed | Effects of high-risk human papillomavirus infection on P53, pRb, and survivin in lung adenocarcinoma—a retrospective study |
| title_short | Effects of high-risk human papillomavirus infection on P53, pRb, and survivin in lung adenocarcinoma—a retrospective study |
| title_sort | effects of high risk human papillomavirus infection on p53 prb and survivin in lung adenocarcinoma a retrospective study |
| topic | Human papillomavirus Lung adenocarcinoma P53 PRb Survivin Smoking |
| url | https://peerj.com/articles/15570.pdf |
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