Assessment of Persistence of Antibodies Detected by Indirect-Elisa <em>Trypanosoma vivax</em> after Trypanocidal Treatment in Cattle Naturally Infected
Determination of infection incidence and prevalence is essential in the epidemiological study of cattle trypanosomoses. These studies are based on direct and indirect diagnostic techniques. Parasitological techniques are of low sensitivity. PCR detection has a better sensitivity, which is not, howev...
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| Format: | Article |
| Language: | English |
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CIRAD
2003-03-01
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| Series: | Revue d’Elevage et de Médecine Vétérinaire des Pays Tropicaux |
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| Online Access: | http://revues.cirad.fr/index.php/REMVT/article/view/9855 |
| _version_ | 1797764075141201920 |
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| author | M. Desquesnes Z. Bengaly M. L. Dia |
| author_facet | M. Desquesnes Z. Bengaly M. L. Dia |
| author_sort | M. Desquesnes |
| collection | DOAJ |
| description | Determination of infection incidence and prevalence is essential in the epidemiological study of cattle trypanosomoses. These studies are based on direct and indirect diagnostic techniques. Parasitological techniques are of low sensitivity. PCR detection has a better sensitivity, which is not, however, sufficient to detect most chronic infections. Detection by indirect-ELISA of antibodies directed against trypanosomes is the best-adapted tool to assess the magnitude of trypanosome infections in cattle populations. However, the detection of antibodies in cattle does not mean the animals are actively infected; determining antibody persistence after curative treatment or natural self cure are essential in analyzing the results. In the present paper, persistence of anti-trypanosome antibodies was evaluated by indirect-ELISA in 32 crossbred cattle (zebu x Baoule) naturally infected by Trypanosoma vivax, after treatment with diminazene aceturate (7 mg/kg i.m.). The initial seroprevalence was 100%; the decline in antibodies started three months later to reach a seroprevalence of 3% five months after treatment. Persistent positive cases were due to the persistence of the infection or to natural reinfection in two animals during the study (they were excluded from the cohort), and to one case unaccounted for. Seroprevalence decreased faster in the young. It is suggested that the seroprevalence by indirect-ELISA against T. vivax four or five months after a curative treatment indicates the prevalence of active infections. |
| first_indexed | 2024-03-12T19:50:32Z |
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| id | doaj.art-eafea4220b96411d812c1854d7258bf8 |
| institution | Directory Open Access Journal |
| issn | 0035-1865 1951-6711 |
| language | English |
| last_indexed | 2024-03-12T19:50:32Z |
| publishDate | 2003-03-01 |
| publisher | CIRAD |
| record_format | Article |
| series | Revue d’Elevage et de Médecine Vétérinaire des Pays Tropicaux |
| spelling | doaj.art-eafea4220b96411d812c1854d7258bf82023-08-02T03:14:39ZengCIRADRevue d’Elevage et de Médecine Vétérinaire des Pays Tropicaux0035-18651951-67112003-03-01563-414114410.19182/remvt.98559855Assessment of Persistence of Antibodies Detected by Indirect-Elisa <em>Trypanosoma vivax</em> after Trypanocidal Treatment in Cattle Naturally InfectedM. Desquesnes0Z. Bengaly1M. L. Dia21- Cirad-emvt, TA30/B, campus international de Baillarguet, 34398 Montpellier Cedex 5, France 2- Cirdes, 01 BP 454, Bobo-Dioulasso 01, Burkina FasoCirdes, 01 BP 454, Bobo-Dioulasso 01, Burkina FasoCirdes, 01 BP 454, Bobo-Dioulasso 01, Burkina FasoDetermination of infection incidence and prevalence is essential in the epidemiological study of cattle trypanosomoses. These studies are based on direct and indirect diagnostic techniques. Parasitological techniques are of low sensitivity. PCR detection has a better sensitivity, which is not, however, sufficient to detect most chronic infections. Detection by indirect-ELISA of antibodies directed against trypanosomes is the best-adapted tool to assess the magnitude of trypanosome infections in cattle populations. However, the detection of antibodies in cattle does not mean the animals are actively infected; determining antibody persistence after curative treatment or natural self cure are essential in analyzing the results. In the present paper, persistence of anti-trypanosome antibodies was evaluated by indirect-ELISA in 32 crossbred cattle (zebu x Baoule) naturally infected by Trypanosoma vivax, after treatment with diminazene aceturate (7 mg/kg i.m.). The initial seroprevalence was 100%; the decline in antibodies started three months later to reach a seroprevalence of 3% five months after treatment. Persistent positive cases were due to the persistence of the infection or to natural reinfection in two animals during the study (they were excluded from the cohort), and to one case unaccounted for. Seroprevalence decreased faster in the young. It is suggested that the seroprevalence by indirect-ELISA against T. vivax four or five months after a curative treatment indicates the prevalence of active infections.http://revues.cirad.fr/index.php/REMVT/article/view/9855BovinTrypanosoma vivaxTest ElisaEpidémiologieBurkina Faso |
| spellingShingle | M. Desquesnes Z. Bengaly M. L. Dia Assessment of Persistence of Antibodies Detected by Indirect-Elisa <em>Trypanosoma vivax</em> after Trypanocidal Treatment in Cattle Naturally Infected Revue d’Elevage et de Médecine Vétérinaire des Pays Tropicaux Bovin Trypanosoma vivax Test Elisa Epidémiologie Burkina Faso |
| title | Assessment of Persistence of Antibodies Detected by Indirect-Elisa <em>Trypanosoma vivax</em> after Trypanocidal Treatment in Cattle Naturally Infected |
| title_full | Assessment of Persistence of Antibodies Detected by Indirect-Elisa <em>Trypanosoma vivax</em> after Trypanocidal Treatment in Cattle Naturally Infected |
| title_fullStr | Assessment of Persistence of Antibodies Detected by Indirect-Elisa <em>Trypanosoma vivax</em> after Trypanocidal Treatment in Cattle Naturally Infected |
| title_full_unstemmed | Assessment of Persistence of Antibodies Detected by Indirect-Elisa <em>Trypanosoma vivax</em> after Trypanocidal Treatment in Cattle Naturally Infected |
| title_short | Assessment of Persistence of Antibodies Detected by Indirect-Elisa <em>Trypanosoma vivax</em> after Trypanocidal Treatment in Cattle Naturally Infected |
| title_sort | assessment of persistence of antibodies detected by indirect elisa em trypanosoma vivax em after trypanocidal treatment in cattle naturally infected |
| topic | Bovin Trypanosoma vivax Test Elisa Epidémiologie Burkina Faso |
| url | http://revues.cirad.fr/index.php/REMVT/article/view/9855 |
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