Maternal protein restriction affects gene expression and enzyme activity of intestinal disaccharidases in adult rat offspring
This study investigated the consequences of intrauterine protein restriction on the gastrointestinal tract and particularly on the gene expression and activity of intestinal disaccharidases in the adult offspring. Wistar rat dams were fed isocaloric diets containing 6% protein (restri...
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
2013-03-01
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| Series: | Brazilian Journal of Medical and Biological Research |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000300287&lng=en&tlng=en |
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| author | D.F. Pinheiro P.D.G. Pacheco P.V. Alvarenga J. Buratini Jr A.C.S. Castilho P.F. Lima D.R.S. Sartori M.L.M. Vicentini-Paulino |
| author_facet | D.F. Pinheiro P.D.G. Pacheco P.V. Alvarenga J. Buratini Jr A.C.S. Castilho P.F. Lima D.R.S. Sartori M.L.M. Vicentini-Paulino |
| author_sort | D.F. Pinheiro |
| collection | DOAJ |
| description | This study investigated the consequences of intrauterine protein restriction on the gastrointestinal tract and particularly on the gene expression and activity of intestinal disaccharidases in the adult offspring. Wistar rat dams were fed isocaloric diets containing 6% protein (restricted, n = 8) or 17% protein (control, n = 8) throughout gestation. Male offspring (n = 5-8 in each group) were evaluated at 3 or 16 weeks of age. Maternal protein restriction during pregnancy produced offspring with growth restriction from birth (5.7 ± 0.1 vs 6.3 ± 0.1 g; mean ± SE) to weaning (42.4 ± 1.3 vs 49.1 ± 1.6 g), although at 16 weeks of age their body weight was similar to control (421.7 ± 8.9 and 428.5 ± 8.5 g). Maternal protein restriction also increased lactase activity in the proximal (0.23 ± 0.02 vs 0.15 ± 0.02), medial (0.30 ± 0.06 vs 0.14 ± 0.01) and distal (0.43 ± 0.07 vs 0.07 ± 0.02 U·g-1·min-1) small intestine, and mRNA lactase abundance in the proximal intestine (7.96 ± 1.11 vs 2.38 ± 0.47 relative units) of 3-week-old offspring rats. In addition, maternal protein restriction increased sucrase activity (1.20 ± 0.02 vs 0.91 ± 0.02 U·g-1·min-1) and sucrase mRNA abundance (4.48 ± 0.51 vs 1.95 ± 0.17 relative units) in the duodenum of 16-week-old rats. In conclusion, the present study shows for the first time that intrauterine protein restriction affects gene expression of intestinal enzymes in offspring. |
| first_indexed | 2024-12-21T22:36:17Z |
| format | Article |
| id | doaj.art-eafee0a4104d448bb54ab9d6324dc899 |
| institution | Directory Open Access Journal |
| issn | 1414-431X |
| language | English |
| last_indexed | 2024-12-21T22:36:17Z |
| publishDate | 2013-03-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-eafee0a4104d448bb54ab9d6324dc8992022-12-21T18:47:58ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research1414-431X2013-03-0146328729210.1590/1414-431X20122561S0100-879X2013000300287Maternal protein restriction affects gene expression and enzyme activity of intestinal disaccharidases in adult rat offspringD.F. PinheiroP.D.G. PachecoP.V. AlvarengaJ. Buratini JrA.C.S. CastilhoP.F. LimaD.R.S. SartoriM.L.M. Vicentini-PaulinoThis study investigated the consequences of intrauterine protein restriction on the gastrointestinal tract and particularly on the gene expression and activity of intestinal disaccharidases in the adult offspring. Wistar rat dams were fed isocaloric diets containing 6% protein (restricted, n = 8) or 17% protein (control, n = 8) throughout gestation. Male offspring (n = 5-8 in each group) were evaluated at 3 or 16 weeks of age. Maternal protein restriction during pregnancy produced offspring with growth restriction from birth (5.7 ± 0.1 vs 6.3 ± 0.1 g; mean ± SE) to weaning (42.4 ± 1.3 vs 49.1 ± 1.6 g), although at 16 weeks of age their body weight was similar to control (421.7 ± 8.9 and 428.5 ± 8.5 g). Maternal protein restriction also increased lactase activity in the proximal (0.23 ± 0.02 vs 0.15 ± 0.02), medial (0.30 ± 0.06 vs 0.14 ± 0.01) and distal (0.43 ± 0.07 vs 0.07 ± 0.02 U·g-1·min-1) small intestine, and mRNA lactase abundance in the proximal intestine (7.96 ± 1.11 vs 2.38 ± 0.47 relative units) of 3-week-old offspring rats. In addition, maternal protein restriction increased sucrase activity (1.20 ± 0.02 vs 0.91 ± 0.02 U·g-1·min-1) and sucrase mRNA abundance (4.48 ± 0.51 vs 1.95 ± 0.17 relative units) in the duodenum of 16-week-old rats. In conclusion, the present study shows for the first time that intrauterine protein restriction affects gene expression of intestinal enzymes in offspring.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000300287&lng=en&tlng=enmRNA enzyme abundanceMaternal protein restrictionDisaccharidase activityFetal programming |
| spellingShingle | D.F. Pinheiro P.D.G. Pacheco P.V. Alvarenga J. Buratini Jr A.C.S. Castilho P.F. Lima D.R.S. Sartori M.L.M. Vicentini-Paulino Maternal protein restriction affects gene expression and enzyme activity of intestinal disaccharidases in adult rat offspring Brazilian Journal of Medical and Biological Research mRNA enzyme abundance Maternal protein restriction Disaccharidase activity Fetal programming |
| title | Maternal protein restriction affects gene expression and enzyme activity of intestinal disaccharidases in adult rat offspring |
| title_full | Maternal protein restriction affects gene expression and enzyme activity of intestinal disaccharidases in adult rat offspring |
| title_fullStr | Maternal protein restriction affects gene expression and enzyme activity of intestinal disaccharidases in adult rat offspring |
| title_full_unstemmed | Maternal protein restriction affects gene expression and enzyme activity of intestinal disaccharidases in adult rat offspring |
| title_short | Maternal protein restriction affects gene expression and enzyme activity of intestinal disaccharidases in adult rat offspring |
| title_sort | maternal protein restriction affects gene expression and enzyme activity of intestinal disaccharidases in adult rat offspring |
| topic | mRNA enzyme abundance Maternal protein restriction Disaccharidase activity Fetal programming |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000300287&lng=en&tlng=en |
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