CRISPR Typing Increases the Discriminatory Power of Streptococcus agalactiae Typing Methods
We explored the relevance of a Clustered regularly interspaced short palindromic repeats (CRISPR)-based genotyping tool for Streptococcus agalactiae typing and we compared this method to current molecular methods [multi locus sequence typing (MLST) and capsular typing]. To this effect, we developed...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-07-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.675597/full |
| _version_ | 1818652171477975040 |
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| author | Clémence Beauruelle Clémence Beauruelle Ludovic Treluyer Adeline Pastuszka Adeline Pastuszka Thierry Cochard Clément Lier Clément Lier Laurent Mereghetti Laurent Mereghetti Philippe Glaser Philippe Glaser Claire Poyart Claire Poyart Claire Poyart Philippe Lanotte |
| author_facet | Clémence Beauruelle Clémence Beauruelle Ludovic Treluyer Adeline Pastuszka Adeline Pastuszka Thierry Cochard Clément Lier Clément Lier Laurent Mereghetti Laurent Mereghetti Philippe Glaser Philippe Glaser Claire Poyart Claire Poyart Claire Poyart Philippe Lanotte |
| author_sort | Clémence Beauruelle |
| collection | DOAJ |
| description | We explored the relevance of a Clustered regularly interspaced short palindromic repeats (CRISPR)-based genotyping tool for Streptococcus agalactiae typing and we compared this method to current molecular methods [multi locus sequence typing (MLST) and capsular typing]. To this effect, we developed two CRISPR marker schemes (using 94 or 25 markers, respectively). Among the 255 S. agalactiae isolates tested, 229 CRISPR profiles were obtained. The 94 and 25 markers made it possible to efficiently separate isolates with a high diversity index (0.9947 and 0.9267, respectively), highlighting a high discriminatory power, superior to that of both capsular typing and MLST (diversity index of 0.9017 for MLST). This method has the advantage of being correlated with MLST [through analysis of the terminal direct repeat (TDR) and ancestral spacers] and to possess a high discriminatory power (through analysis of the leader-end spacers recently acquired, which are the witnesses of genetic mobile elements encountered by the bacteria). Furthermore, this “one-shot” approach presents the benefit of much-reduced time and cost in comparison with MLST. On the basis of these data, we propose that this method could become a reference method for group B Streptococcus (GBS) typing. |
| first_indexed | 2024-12-17T02:17:46Z |
| format | Article |
| id | doaj.art-eb00eac62a4a41deb22132053c568f6e |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-17T02:17:46Z |
| publishDate | 2021-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-eb00eac62a4a41deb22132053c568f6e2022-12-21T22:07:21ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-07-011210.3389/fmicb.2021.675597675597CRISPR Typing Increases the Discriminatory Power of Streptococcus agalactiae Typing MethodsClémence Beauruelle0Clémence Beauruelle1Ludovic Treluyer2Adeline Pastuszka3Adeline Pastuszka4Thierry Cochard5Clément Lier6Clément Lier7Laurent Mereghetti8Laurent Mereghetti9Philippe Glaser10Philippe Glaser11Claire Poyart12Claire Poyart13Claire Poyart14Philippe Lanotte15Département de Bactériologie-Virologie, Hygiène Hospitalière et Parasitologie-Mycologie, Centre Hospitalier Régional Universitaire (CHRU) de Brest, Brest, FranceInserm, EFS, UMR 1078, GGB, Universitè de Bretagne Occidentale, Brest, FranceInstitut Cochin, Team Bacteria and Perinatality, INSERM U1016, Paris, FranceINRAE, ISP, Université de Tours, Tours, FranceService de Bactériologie-Virologie, CHRU de Tours, Tours, FranceINRAE, ISP, Université de Tours, Tours, FranceINRAE, ISP, Université de Tours, Tours, FranceService de Bactériologie-Virologie, CHRU de Tours, Tours, FranceINRAE, ISP, Université de Tours, Tours, FranceService de Bactériologie-Virologie, CHRU de Tours, Tours, FranceEvolution and Ecology of Resistance to Antibiotics (EERA) Unit, Institut Pasteur, Paris, FranceUMR CNRS 3525, Paris, FranceInstitut Cochin, Team Bacteria and Perinatality, INSERM U1016, Paris, FranceCNRS UMR 8104, Paris Descartes University, Paris, FranceDepartment of Bacteriology, University Hospitals Paris Centre-Cochin, Assistance Publique-Hôpitaux de Paris, Paris, FranceINRAE, ISP, Université de Tours, Tours, FranceWe explored the relevance of a Clustered regularly interspaced short palindromic repeats (CRISPR)-based genotyping tool for Streptococcus agalactiae typing and we compared this method to current molecular methods [multi locus sequence typing (MLST) and capsular typing]. To this effect, we developed two CRISPR marker schemes (using 94 or 25 markers, respectively). Among the 255 S. agalactiae isolates tested, 229 CRISPR profiles were obtained. The 94 and 25 markers made it possible to efficiently separate isolates with a high diversity index (0.9947 and 0.9267, respectively), highlighting a high discriminatory power, superior to that of both capsular typing and MLST (diversity index of 0.9017 for MLST). This method has the advantage of being correlated with MLST [through analysis of the terminal direct repeat (TDR) and ancestral spacers] and to possess a high discriminatory power (through analysis of the leader-end spacers recently acquired, which are the witnesses of genetic mobile elements encountered by the bacteria). Furthermore, this “one-shot” approach presents the benefit of much-reduced time and cost in comparison with MLST. On the basis of these data, we propose that this method could become a reference method for group B Streptococcus (GBS) typing.https://www.frontiersin.org/articles/10.3389/fmicb.2021.675597/fullCRISPR-Casgroup B StreptococcusStreptococcus agalactiaetypingmolecular subtyping |
| spellingShingle | Clémence Beauruelle Clémence Beauruelle Ludovic Treluyer Adeline Pastuszka Adeline Pastuszka Thierry Cochard Clément Lier Clément Lier Laurent Mereghetti Laurent Mereghetti Philippe Glaser Philippe Glaser Claire Poyart Claire Poyart Claire Poyart Philippe Lanotte CRISPR Typing Increases the Discriminatory Power of Streptococcus agalactiae Typing Methods Frontiers in Microbiology CRISPR-Cas group B Streptococcus Streptococcus agalactiae typing molecular subtyping |
| title | CRISPR Typing Increases the Discriminatory Power of Streptococcus agalactiae Typing Methods |
| title_full | CRISPR Typing Increases the Discriminatory Power of Streptococcus agalactiae Typing Methods |
| title_fullStr | CRISPR Typing Increases the Discriminatory Power of Streptococcus agalactiae Typing Methods |
| title_full_unstemmed | CRISPR Typing Increases the Discriminatory Power of Streptococcus agalactiae Typing Methods |
| title_short | CRISPR Typing Increases the Discriminatory Power of Streptococcus agalactiae Typing Methods |
| title_sort | crispr typing increases the discriminatory power of streptococcus agalactiae typing methods |
| topic | CRISPR-Cas group B Streptococcus Streptococcus agalactiae typing molecular subtyping |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.675597/full |
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