The Bag Method for Islet Cell Infusion
As islet cell transplantation gains increasing interest following results published by the Edmonton group, results that have been successfully reproduced by several centers nationwide and abroad, the need of guidelines to standardize the procedure becomes highly important. We detail the key steps of...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2003-10-01
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| Series: | Cell Transplantation |
| Online Access: | https://doi.org/10.3727/000000003108747280 |
| _version_ | 1818414278060802048 |
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| author | David A. Baidal Tatiana Froud Jacqueline V. Ferreira Aisha Khan Rodolfo Alejandro Camillo Ricordi M.D. |
| author_facet | David A. Baidal Tatiana Froud Jacqueline V. Ferreira Aisha Khan Rodolfo Alejandro Camillo Ricordi M.D. |
| author_sort | David A. Baidal |
| collection | DOAJ |
| description | As islet cell transplantation gains increasing interest following results published by the Edmonton group, results that have been successfully reproduced by several centers nationwide and abroad, the need of guidelines to standardize the procedure becomes highly important. We detail the key steps of the infusion procedure utilizing a closed gravity fed bag system utilized at our institution since 1990, which consists of a 600-ml transfer bag and a 150-ml rinse bag connected via sterile tubing. The use of gravity allows for a control rate of infusion as well as providing a safety mechanism through natural reduction of flow that parallels any increase in portal pressure, therefore allowing the operator to prevent precipitous pressure rises. Reports on significant rise in portal pressures during islet cell infusion as well as portal vein thrombosis have been published. Infusion at these centers was carried out using a syringe method. Using our technique, portal vein thrombosis (partial or complete) was not detected in any of the infusions performed at our institution. This method may be of assistance to minimize some of the observed complications associated with islet transplant procedures and has now been adapted by most centers performing clinical islet transplantation. |
| first_indexed | 2024-12-14T11:16:33Z |
| format | Article |
| id | doaj.art-eb03adff107e4742b4f6af54e18ea98f |
| institution | Directory Open Access Journal |
| issn | 0963-6897 1555-3892 |
| language | English |
| last_indexed | 2024-12-14T11:16:33Z |
| publishDate | 2003-10-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Cell Transplantation |
| spelling | doaj.art-eb03adff107e4742b4f6af54e18ea98f2022-12-21T23:04:00ZengSAGE PublishingCell Transplantation0963-68971555-38922003-10-011210.3727/000000003108747280The Bag Method for Islet Cell InfusionDavid A. Baidal0Tatiana Froud1Jacqueline V. Ferreira2Aisha Khan3Rodolfo Alejandro4Camillo Ricordi M.D.5Diabetes Research Institute, University of Miami School of Medicine, Miami, FL 33136Diabetes Research Institute, University of Miami School of Medicine, Miami, FL 33136Diabetes Research Institute, University of Miami School of Medicine, Miami, FL 33136Diabetes Research Institute, University of Miami School of Medicine, Miami, FL 33136Diabetes Research Institute, University of Miami School of Medicine, Miami, FL 33136Diabetes Research Institute, University of Miami School of Medicine, Miami, FL 33136As islet cell transplantation gains increasing interest following results published by the Edmonton group, results that have been successfully reproduced by several centers nationwide and abroad, the need of guidelines to standardize the procedure becomes highly important. We detail the key steps of the infusion procedure utilizing a closed gravity fed bag system utilized at our institution since 1990, which consists of a 600-ml transfer bag and a 150-ml rinse bag connected via sterile tubing. The use of gravity allows for a control rate of infusion as well as providing a safety mechanism through natural reduction of flow that parallels any increase in portal pressure, therefore allowing the operator to prevent precipitous pressure rises. Reports on significant rise in portal pressures during islet cell infusion as well as portal vein thrombosis have been published. Infusion at these centers was carried out using a syringe method. Using our technique, portal vein thrombosis (partial or complete) was not detected in any of the infusions performed at our institution. This method may be of assistance to minimize some of the observed complications associated with islet transplant procedures and has now been adapted by most centers performing clinical islet transplantation.https://doi.org/10.3727/000000003108747280 |
| spellingShingle | David A. Baidal Tatiana Froud Jacqueline V. Ferreira Aisha Khan Rodolfo Alejandro Camillo Ricordi M.D. The Bag Method for Islet Cell Infusion Cell Transplantation |
| title | The Bag Method for Islet Cell Infusion |
| title_full | The Bag Method for Islet Cell Infusion |
| title_fullStr | The Bag Method for Islet Cell Infusion |
| title_full_unstemmed | The Bag Method for Islet Cell Infusion |
| title_short | The Bag Method for Islet Cell Infusion |
| title_sort | bag method for islet cell infusion |
| url | https://doi.org/10.3727/000000003108747280 |
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