ASCA-related antibodies in the blood sera of healthy donors and patients with colorectal cancer: characterization with oligosaccharides related to Saccharomyces cerevisiae mannan
Mannans are polysaccharide antigens expressed on the cell wall of different fungal species including Saccharomyces cerevisiae and Candida spp. These fungi are components of the normal intestinal microflora, and the presence of antibodies to fungal antigens is known to reflect the features of the pat...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-12-01
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| Series: | Frontiers in Molecular Biosciences |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmolb.2023.1296828/full |
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| author | Vadim B. Krylov Vadim B. Krylov Anton N. Kuznetsov Alina V. Polyanskaya Pavel V. Tsarapaev Pavel V. Tsarapaev Dmitry V. Yashunsky Nikolay E. Kushlinskii Nikolay E. Nifantiev |
| author_facet | Vadim B. Krylov Vadim B. Krylov Anton N. Kuznetsov Alina V. Polyanskaya Pavel V. Tsarapaev Pavel V. Tsarapaev Dmitry V. Yashunsky Nikolay E. Kushlinskii Nikolay E. Nifantiev |
| author_sort | Vadim B. Krylov |
| collection | DOAJ |
| description | Mannans are polysaccharide antigens expressed on the cell wall of different fungal species including Saccharomyces cerevisiae and Candida spp. These fungi are components of the normal intestinal microflora, and the presence of antibodies to fungal antigens is known to reflect the features of the patient’s immune system. Thus, titers of IgG and IgA antibodies against Saccharomyces cerevisiae mannan (ASCA) are markers for clinical diagnostics of inflammatory bowel diseases. The complex organization and heterogeneity of cell-wall mannans may reduce the quality and reproducibility of ELISA results due to interference by different antigenic epitopes. In this research, we analyzed the levels of IgG antibodies in the sera of healthy donors and patients with colorectal cancer using an array of synthetic oligosaccharides related to distinct fragments of fungal mannan. This study aimed to establish the influence of oligosaccharide structure on their antigenicity. Variations in the structure of the previously established ASCA epitope (changing type of linkage, chain length, and the presence of branches) significantly modified the ability of ligands to bind to circulating antibodies in blood sera. The study showed that surface presentation density of the ligand critically affects the results of enzyme immunoassay. The transition from natural coating antigens to their corresponding synthetic mimetics with a defined structure opens new opportunities for improving existing ELISA test systems, as well as developing diagnostic kits with new properties. |
| first_indexed | 2024-03-09T02:10:16Z |
| format | Article |
| id | doaj.art-eb06535752d64760b121aaeebae9a017 |
| institution | Directory Open Access Journal |
| issn | 2296-889X |
| language | English |
| last_indexed | 2024-03-09T02:10:16Z |
| publishDate | 2023-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Molecular Biosciences |
| spelling | doaj.art-eb06535752d64760b121aaeebae9a0172023-12-07T13:01:58ZengFrontiers Media S.A.Frontiers in Molecular Biosciences2296-889X2023-12-011010.3389/fmolb.2023.12968281296828ASCA-related antibodies in the blood sera of healthy donors and patients with colorectal cancer: characterization with oligosaccharides related to Saccharomyces cerevisiae mannanVadim B. Krylov0Vadim B. Krylov1Anton N. Kuznetsov2Alina V. Polyanskaya3Pavel V. Tsarapaev4Pavel V. Tsarapaev5Dmitry V. Yashunsky6Nikolay E. Kushlinskii7Nikolay E. Nifantiev8Laboratory of Synthetic Glycovaccines, N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, RussiaLaboratory of Glycoconjugate Chemistry, N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, RussiaLaboratory of Synthetic Glycovaccines, N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, RussiaLaboratory of Synthetic Glycovaccines, N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, RussiaLaboratory of Synthetic Glycovaccines, N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, RussiaN. N. Blokhin National Medical Research Center of Oncology, Ministry of Health of the Russian Federation, Moscow, RussiaLaboratory of Glycoconjugate Chemistry, N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, RussiaN. N. Blokhin National Medical Research Center of Oncology, Ministry of Health of the Russian Federation, Moscow, RussiaLaboratory of Glycoconjugate Chemistry, N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, RussiaMannans are polysaccharide antigens expressed on the cell wall of different fungal species including Saccharomyces cerevisiae and Candida spp. These fungi are components of the normal intestinal microflora, and the presence of antibodies to fungal antigens is known to reflect the features of the patient’s immune system. Thus, titers of IgG and IgA antibodies against Saccharomyces cerevisiae mannan (ASCA) are markers for clinical diagnostics of inflammatory bowel diseases. The complex organization and heterogeneity of cell-wall mannans may reduce the quality and reproducibility of ELISA results due to interference by different antigenic epitopes. In this research, we analyzed the levels of IgG antibodies in the sera of healthy donors and patients with colorectal cancer using an array of synthetic oligosaccharides related to distinct fragments of fungal mannan. This study aimed to establish the influence of oligosaccharide structure on their antigenicity. Variations in the structure of the previously established ASCA epitope (changing type of linkage, chain length, and the presence of branches) significantly modified the ability of ligands to bind to circulating antibodies in blood sera. The study showed that surface presentation density of the ligand critically affects the results of enzyme immunoassay. The transition from natural coating antigens to their corresponding synthetic mimetics with a defined structure opens new opportunities for improving existing ELISA test systems, as well as developing diagnostic kits with new properties.https://www.frontiersin.org/articles/10.3389/fmolb.2023.1296828/fullmannanSaccharomyces cerevisiaeCandida albicansfungiantibodiesIgG |
| spellingShingle | Vadim B. Krylov Vadim B. Krylov Anton N. Kuznetsov Alina V. Polyanskaya Pavel V. Tsarapaev Pavel V. Tsarapaev Dmitry V. Yashunsky Nikolay E. Kushlinskii Nikolay E. Nifantiev ASCA-related antibodies in the blood sera of healthy donors and patients with colorectal cancer: characterization with oligosaccharides related to Saccharomyces cerevisiae mannan Frontiers in Molecular Biosciences mannan Saccharomyces cerevisiae Candida albicans fungi antibodies IgG |
| title | ASCA-related antibodies in the blood sera of healthy donors and patients with colorectal cancer: characterization with oligosaccharides related to Saccharomyces cerevisiae mannan |
| title_full | ASCA-related antibodies in the blood sera of healthy donors and patients with colorectal cancer: characterization with oligosaccharides related to Saccharomyces cerevisiae mannan |
| title_fullStr | ASCA-related antibodies in the blood sera of healthy donors and patients with colorectal cancer: characterization with oligosaccharides related to Saccharomyces cerevisiae mannan |
| title_full_unstemmed | ASCA-related antibodies in the blood sera of healthy donors and patients with colorectal cancer: characterization with oligosaccharides related to Saccharomyces cerevisiae mannan |
| title_short | ASCA-related antibodies in the blood sera of healthy donors and patients with colorectal cancer: characterization with oligosaccharides related to Saccharomyces cerevisiae mannan |
| title_sort | asca related antibodies in the blood sera of healthy donors and patients with colorectal cancer characterization with oligosaccharides related to saccharomyces cerevisiae mannan |
| topic | mannan Saccharomyces cerevisiae Candida albicans fungi antibodies IgG |
| url | https://www.frontiersin.org/articles/10.3389/fmolb.2023.1296828/full |
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