Simultaneous detection and differentiation of canine parvovirus and feline parvovirus by high resolution melting analysis
Abstract Background Canine parvovirus (CPV) and feline parvovirus (FPV) are causative agents of diarrhea in dogs and cats, which manifests as depression, vomiting, fever, loss of appetite, leucopenia, and diarrhea in young animals. CPV and FPV can single or mixed infect cats and cause disease. To di...
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| Format: | Article |
| Language: | English |
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BMC
2019-05-01
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| Series: | BMC Veterinary Research |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12917-019-1898-5 |
| _version_ | 1818383764467744768 |
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| author | Yaru Sun Yuening Cheng Peng Lin Hewei Zhang Li Yi Mingwei Tong Zhigang Cao Shuang Li Shipeng Cheng Jianke Wang |
| author_facet | Yaru Sun Yuening Cheng Peng Lin Hewei Zhang Li Yi Mingwei Tong Zhigang Cao Shuang Li Shipeng Cheng Jianke Wang |
| author_sort | Yaru Sun |
| collection | DOAJ |
| description | Abstract Background Canine parvovirus (CPV) and feline parvovirus (FPV) are causative agents of diarrhea in dogs and cats, which manifests as depression, vomiting, fever, loss of appetite, leucopenia, and diarrhea in young animals. CPV and FPV can single or mixed infect cats and cause disease. To diagnose sick animals effectively, an effective virus diagnostic and genome typing method with high sensitivity and specificity is required. Results In this study, a conserved segment containing one SNP A4408C of parvovirus was used for real-time PCR amplification. Subsequently, data were auto-analyzed and plotted using Applied Biosystems® High Resolution Melt Software v3.1. Results showed that CPV and FPV can be detected simultaneously in a single PCR reaction. No cross-reactions were observed with canine adenovirus, canine coronavirus, and canine distemper virus. The assay had a detection limit of 4.2 genome copies of CPV and FPV. A total of 80 clinical samples were subjected to this assay, as well as to conventional PCR-sequence assay and virus isolation. Results showed that the percentage of agreement of the assay and other methods are high. Conclusions In short, we have developed a diagnostic test for the accurate detection and differentiation of CPV and FPV in fecal samples, which is also cost effective. |
| first_indexed | 2024-12-14T03:11:33Z |
| format | Article |
| id | doaj.art-eb07b3ca59c7429eb7aa58ef35476049 |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-12-14T03:11:33Z |
| publishDate | 2019-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-eb07b3ca59c7429eb7aa58ef354760492022-12-21T23:19:16ZengBMCBMC Veterinary Research1746-61482019-05-011511810.1186/s12917-019-1898-5Simultaneous detection and differentiation of canine parvovirus and feline parvovirus by high resolution melting analysisYaru Sun0Yuening Cheng1Peng Lin2Hewei Zhang3Li Yi4Mingwei Tong5Zhigang Cao6Shuang Li7Shipeng Cheng8Jianke Wang9Key Laboratory of Special Animal Epidemic Disease, Ministry of AgricultureKey Laboratory of Special Animal Epidemic Disease, Ministry of AgricultureKey Laboratory of Special Animal Epidemic Disease, Ministry of AgricultureKey Laboratory of Special Animal Epidemic Disease, Ministry of AgricultureKey Laboratory of Special Animal Epidemic Disease, Ministry of AgricultureKey Laboratory of Special Animal Epidemic Disease, Ministry of AgricultureKey Laboratory of Special Animal Epidemic Disease, Ministry of AgricultureKey Laboratory of Special Animal Epidemic Disease, Ministry of AgricultureKey Laboratory of Special Animal Epidemic Disease, Ministry of AgricultureKey Laboratory of Special Animal Epidemic Disease, Ministry of AgricultureAbstract Background Canine parvovirus (CPV) and feline parvovirus (FPV) are causative agents of diarrhea in dogs and cats, which manifests as depression, vomiting, fever, loss of appetite, leucopenia, and diarrhea in young animals. CPV and FPV can single or mixed infect cats and cause disease. To diagnose sick animals effectively, an effective virus diagnostic and genome typing method with high sensitivity and specificity is required. Results In this study, a conserved segment containing one SNP A4408C of parvovirus was used for real-time PCR amplification. Subsequently, data were auto-analyzed and plotted using Applied Biosystems® High Resolution Melt Software v3.1. Results showed that CPV and FPV can be detected simultaneously in a single PCR reaction. No cross-reactions were observed with canine adenovirus, canine coronavirus, and canine distemper virus. The assay had a detection limit of 4.2 genome copies of CPV and FPV. A total of 80 clinical samples were subjected to this assay, as well as to conventional PCR-sequence assay and virus isolation. Results showed that the percentage of agreement of the assay and other methods are high. Conclusions In short, we have developed a diagnostic test for the accurate detection and differentiation of CPV and FPV in fecal samples, which is also cost effective.http://link.springer.com/article/10.1186/s12917-019-1898-5Simultaneous detectionDifferentiationCanine parvovirusFeline parvovirusHRM |
| spellingShingle | Yaru Sun Yuening Cheng Peng Lin Hewei Zhang Li Yi Mingwei Tong Zhigang Cao Shuang Li Shipeng Cheng Jianke Wang Simultaneous detection and differentiation of canine parvovirus and feline parvovirus by high resolution melting analysis BMC Veterinary Research Simultaneous detection Differentiation Canine parvovirus Feline parvovirus HRM |
| title | Simultaneous detection and differentiation of canine parvovirus and feline parvovirus by high resolution melting analysis |
| title_full | Simultaneous detection and differentiation of canine parvovirus and feline parvovirus by high resolution melting analysis |
| title_fullStr | Simultaneous detection and differentiation of canine parvovirus and feline parvovirus by high resolution melting analysis |
| title_full_unstemmed | Simultaneous detection and differentiation of canine parvovirus and feline parvovirus by high resolution melting analysis |
| title_short | Simultaneous detection and differentiation of canine parvovirus and feline parvovirus by high resolution melting analysis |
| title_sort | simultaneous detection and differentiation of canine parvovirus and feline parvovirus by high resolution melting analysis |
| topic | Simultaneous detection Differentiation Canine parvovirus Feline parvovirus HRM |
| url | http://link.springer.com/article/10.1186/s12917-019-1898-5 |
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