Determination of Equilibration Time of Stallion’ Semen Freezing with Extender Skim Milk
The objectives of this experiment were to determine the best equilibration time with extender skim milk in order to maintain the quality of frozen stallion semen. The semen was collected from three 5-8 years old stallions using artificial vagina. The semen characteristics and quality were evaluate...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universitas Jenderal Soedirman (UNSOED), Faculty of Animal Science
2007-09-01
|
| Series: | Animal Production: Indonesian Journal of Animal Production |
| Online Access: | http://animalproduction.net/index.php/JAP/article/view/170 |
| _version_ | 1827299623276904448 |
|---|---|
| author | RI Arifiantini I Supriatna Samsurizal Samsurizal |
| author_facet | RI Arifiantini I Supriatna Samsurizal Samsurizal |
| author_sort | RI Arifiantini |
| collection | DOAJ |
| description | The objectives of this experiment were to determine the best equilibration time with extender skim milk in order to maintain the quality of frozen stallion semen. The semen was collected from three 5-8 years old stallions using artificial vagina. The semen characteristics and quality were evaluated macro- and microscopically and extended with skim milk extender (1:1). The semen was centrifuged at 3000 rpm for 15 minutes. Supernatant was removed and the pellet was re-extended with skim milk extender with 5% glycerol. The extended semen then packed in mini straw (0.5 mL), equilibrated at 5oC for 1 hr (E1), 2 hrs (E2) and 3 hrs (E3), frozen in the liquid nitrogen (N2) vapor for 15 minutes and then stored in liquid N2 containerfor 24 hrs. The straw than thawed at 37oC for 30 seconds. The percentages of sperm motility and viability were observed. The result of this research showed that there were no significant differences between equilibration at 1, 2 and 3 hrs on the percentages of sperm motility with the average of post thawed motility was 23.60 ± 7.10%. The equilibration time affected the sperm viability (P<0.05) in all stallions, with the percentages of viable sperm between 38.10 % and 46.50%. (Animal Production 9(2): 145-152 (2007)
Key Words: Equilibration, frozen semen, stallion |
| first_indexed | 2024-04-24T15:36:53Z |
| format | Article |
| id | doaj.art-eb0b3e52254448b49301743c9e27f466 |
| institution | Directory Open Access Journal |
| issn | 2541-5875 2541-5875 |
| language | English |
| last_indexed | 2024-04-24T15:36:53Z |
| publishDate | 2007-09-01 |
| publisher | Universitas Jenderal Soedirman (UNSOED), Faculty of Animal Science |
| record_format | Article |
| series | Animal Production: Indonesian Journal of Animal Production |
| spelling | doaj.art-eb0b3e52254448b49301743c9e27f4662024-04-02T01:59:37ZengUniversitas Jenderal Soedirman (UNSOED), Faculty of Animal ScienceAnimal Production: Indonesian Journal of Animal Production2541-58752541-58752007-09-0193157Determination of Equilibration Time of Stallion’ Semen Freezing with Extender Skim MilkRI ArifiantiniI SupriatnaSamsurizal SamsurizalThe objectives of this experiment were to determine the best equilibration time with extender skim milk in order to maintain the quality of frozen stallion semen. The semen was collected from three 5-8 years old stallions using artificial vagina. The semen characteristics and quality were evaluated macro- and microscopically and extended with skim milk extender (1:1). The semen was centrifuged at 3000 rpm for 15 minutes. Supernatant was removed and the pellet was re-extended with skim milk extender with 5% glycerol. The extended semen then packed in mini straw (0.5 mL), equilibrated at 5oC for 1 hr (E1), 2 hrs (E2) and 3 hrs (E3), frozen in the liquid nitrogen (N2) vapor for 15 minutes and then stored in liquid N2 containerfor 24 hrs. The straw than thawed at 37oC for 30 seconds. The percentages of sperm motility and viability were observed. The result of this research showed that there were no significant differences between equilibration at 1, 2 and 3 hrs on the percentages of sperm motility with the average of post thawed motility was 23.60 ± 7.10%. The equilibration time affected the sperm viability (P<0.05) in all stallions, with the percentages of viable sperm between 38.10 % and 46.50%. (Animal Production 9(2): 145-152 (2007) Key Words: Equilibration, frozen semen, stallionhttp://animalproduction.net/index.php/JAP/article/view/170 |
| spellingShingle | RI Arifiantini I Supriatna Samsurizal Samsurizal Determination of Equilibration Time of Stallion’ Semen Freezing with Extender Skim Milk Animal Production: Indonesian Journal of Animal Production |
| title | Determination of Equilibration Time of Stallion’ Semen Freezing with Extender Skim Milk |
| title_full | Determination of Equilibration Time of Stallion’ Semen Freezing with Extender Skim Milk |
| title_fullStr | Determination of Equilibration Time of Stallion’ Semen Freezing with Extender Skim Milk |
| title_full_unstemmed | Determination of Equilibration Time of Stallion’ Semen Freezing with Extender Skim Milk |
| title_short | Determination of Equilibration Time of Stallion’ Semen Freezing with Extender Skim Milk |
| title_sort | determination of equilibration time of stallion semen freezing with extender skim milk |
| url | http://animalproduction.net/index.php/JAP/article/view/170 |
| work_keys_str_mv | AT riarifiantini determinationofequilibrationtimeofstallionsemenfreezingwithextenderskimmilk AT isupriatna determinationofequilibrationtimeofstallionsemenfreezingwithextenderskimmilk AT samsurizalsamsurizal determinationofequilibrationtimeofstallionsemenfreezingwithextenderskimmilk |