Proline transporters ProT and PutP are required for Staphylococcus aureus infection.
Proline acquired via specific transporters can serve as a proteinogenic substrate, carbon and nitrogen source, or osmolyte. Previous reports have documented that Staphylococcus aureus, a major community and nosocomial pathogen, encodes at least four proline transporters, PutP, OpuC, OpuD, and ProP....
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2023-01-01
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| Series: | PLoS Pathogens |
| Online Access: | https://doi.org/10.1371/journal.ppat.1011098 |
| _version_ | 1797843276674367488 |
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| author | McKenzie K Lehman Natalie A Sturd Fareha Razvi Dianne L Wellems Steven D Carson Paul D Fey |
| author_facet | McKenzie K Lehman Natalie A Sturd Fareha Razvi Dianne L Wellems Steven D Carson Paul D Fey |
| author_sort | McKenzie K Lehman |
| collection | DOAJ |
| description | Proline acquired via specific transporters can serve as a proteinogenic substrate, carbon and nitrogen source, or osmolyte. Previous reports have documented that Staphylococcus aureus, a major community and nosocomial pathogen, encodes at least four proline transporters, PutP, OpuC, OpuD, and ProP. A combination of genetic approaches and 3H-proline transport assays reveal that a previously unrecognized transporter, ProT, in addition to PutP, are the major proline transporters in S. aureus. Complementation experiments using constitutively expressed non-cognate promoters found that proline transport via OpuD, OpuC, and ProP is minimal. Both proline biosynthesis from arginine and proline transport via ProT are critical for growth when S. aureus is grown under conditions of high salinity. Further, proline transport mediated by ProT or PutP are required for growth in media with and without glucose, indicating both transporters function to acquire proline for proteinogenic purposes in addition to acquisition of proline as a carbon/nitrogen source. Lastly, inactivation of proT and putP resulted in a significant reduction (5 log10) of bacterial burden in murine skin-and-soft tissue infection and bacteremia models, suggesting that proline transport is required to establish a S. aureus infection. |
| first_indexed | 2024-04-09T17:02:33Z |
| format | Article |
| id | doaj.art-eb0ba8c5f301478691573e1d92982e83 |
| institution | Directory Open Access Journal |
| issn | 1553-7366 1553-7374 |
| language | English |
| last_indexed | 2024-04-09T17:02:33Z |
| publishDate | 2023-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj.art-eb0ba8c5f301478691573e1d92982e832023-04-21T05:31:48ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742023-01-01191e101109810.1371/journal.ppat.1011098Proline transporters ProT and PutP are required for Staphylococcus aureus infection.McKenzie K LehmanNatalie A SturdFareha RazviDianne L WellemsSteven D CarsonPaul D FeyProline acquired via specific transporters can serve as a proteinogenic substrate, carbon and nitrogen source, or osmolyte. Previous reports have documented that Staphylococcus aureus, a major community and nosocomial pathogen, encodes at least four proline transporters, PutP, OpuC, OpuD, and ProP. A combination of genetic approaches and 3H-proline transport assays reveal that a previously unrecognized transporter, ProT, in addition to PutP, are the major proline transporters in S. aureus. Complementation experiments using constitutively expressed non-cognate promoters found that proline transport via OpuD, OpuC, and ProP is minimal. Both proline biosynthesis from arginine and proline transport via ProT are critical for growth when S. aureus is grown under conditions of high salinity. Further, proline transport mediated by ProT or PutP are required for growth in media with and without glucose, indicating both transporters function to acquire proline for proteinogenic purposes in addition to acquisition of proline as a carbon/nitrogen source. Lastly, inactivation of proT and putP resulted in a significant reduction (5 log10) of bacterial burden in murine skin-and-soft tissue infection and bacteremia models, suggesting that proline transport is required to establish a S. aureus infection.https://doi.org/10.1371/journal.ppat.1011098 |
| spellingShingle | McKenzie K Lehman Natalie A Sturd Fareha Razvi Dianne L Wellems Steven D Carson Paul D Fey Proline transporters ProT and PutP are required for Staphylococcus aureus infection. PLoS Pathogens |
| title | Proline transporters ProT and PutP are required for Staphylococcus aureus infection. |
| title_full | Proline transporters ProT and PutP are required for Staphylococcus aureus infection. |
| title_fullStr | Proline transporters ProT and PutP are required for Staphylococcus aureus infection. |
| title_full_unstemmed | Proline transporters ProT and PutP are required for Staphylococcus aureus infection. |
| title_short | Proline transporters ProT and PutP are required for Staphylococcus aureus infection. |
| title_sort | proline transporters prot and putp are required for staphylococcus aureus infection |
| url | https://doi.org/10.1371/journal.ppat.1011098 |
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