Prevalence and genotypic characterization of human parvovirus B19 in hemophilia patients
Background and Objectives: Parvovirus B19 (B19V) is usually transmitted through respiratory tract, but can also be received through blood transfusion. This study evaluated the seroprevalence, DNA existence, and circulating genotypes of B19V in hemophilia patients. Materials and Methods: Serum sampl...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2022-08-01
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| Series: | Iranian Journal of Microbiology |
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| Online Access: | https://ijm.tums.ac.ir/index.php/ijm/article/view/3688 |
| _version_ | 1798042091594448896 |
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| author | Mohsen Keshavarz Nahid Janati-Namin Yaser Arjeini Talat Mokhtari-Azad Farhad Rezaei |
| author_facet | Mohsen Keshavarz Nahid Janati-Namin Yaser Arjeini Talat Mokhtari-Azad Farhad Rezaei |
| author_sort | Mohsen Keshavarz |
| collection | DOAJ |
| description |
Background and Objectives: Parvovirus B19 (B19V) is usually transmitted through respiratory tract, but can also be received through blood transfusion. This study evaluated the seroprevalence, DNA existence, and circulating genotypes of B19V in hemophilia patients.
Materials and Methods: Serum samples of cases and controls were analyzed for B19V using ELISA and real-time PCR. Finally, obtained sequences were used for genotyping.
Results: Among cases, 3% were anti-B19V IgM positive and 47% were anti-B19V IgG positive and B19V DNA was detected in 16% of them. However, among controls, 38% were anti-B19V IgG positive (P>0.05) and 5% were B19V DNA positive (P= 0.019). Also ~13% of cases were positive and all of controls were negative for IgG avidity test (P= 0.029). Viral load in case group was higher than control group (P = 0.037).
Conclusion: Since hemophilia patients receive large amounts of blood factors, prevalence of B19V in these patients might be higher than normal subjects.
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| first_indexed | 2024-04-11T22:30:40Z |
| format | Article |
| id | doaj.art-eb0d14286c1a4bc5984e7e56ae7f5dec |
| institution | Directory Open Access Journal |
| issn | 2008-3289 2008-4447 |
| language | English |
| last_indexed | 2024-04-11T22:30:40Z |
| publishDate | 2022-08-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Microbiology |
| spelling | doaj.art-eb0d14286c1a4bc5984e7e56ae7f5dec2022-12-22T03:59:26ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472022-08-0114410.18502/ijm.v14i4.10244Prevalence and genotypic characterization of human parvovirus B19 in hemophilia patientsMohsen Keshavarz0Nahid Janati-Namin1Yaser Arjeini2Talat Mokhtari-Azad3Farhad Rezaei4The Persian Gulf Tropical Medicine Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, IranDepartment of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, IranDepartment of Research and Development, Production and Research Complex, Pasteur Institute of Iran, Tehran, IranDepartment of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, IranDepartment of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Background and Objectives: Parvovirus B19 (B19V) is usually transmitted through respiratory tract, but can also be received through blood transfusion. This study evaluated the seroprevalence, DNA existence, and circulating genotypes of B19V in hemophilia patients. Materials and Methods: Serum samples of cases and controls were analyzed for B19V using ELISA and real-time PCR. Finally, obtained sequences were used for genotyping. Results: Among cases, 3% were anti-B19V IgM positive and 47% were anti-B19V IgG positive and B19V DNA was detected in 16% of them. However, among controls, 38% were anti-B19V IgG positive (P>0.05) and 5% were B19V DNA positive (P= 0.019). Also ~13% of cases were positive and all of controls were negative for IgG avidity test (P= 0.029). Viral load in case group was higher than control group (P = 0.037). Conclusion: Since hemophilia patients receive large amounts of blood factors, prevalence of B19V in these patients might be higher than normal subjects. https://ijm.tums.ac.ir/index.php/ijm/article/view/3688Human parvovirus B19;Hemophilia;Seroprevalence;Blood transfusion;Blood disease;Iran |
| spellingShingle | Mohsen Keshavarz Nahid Janati-Namin Yaser Arjeini Talat Mokhtari-Azad Farhad Rezaei Prevalence and genotypic characterization of human parvovirus B19 in hemophilia patients Iranian Journal of Microbiology Human parvovirus B19; Hemophilia; Seroprevalence; Blood transfusion; Blood disease; Iran |
| title | Prevalence and genotypic characterization of human parvovirus B19 in hemophilia patients |
| title_full | Prevalence and genotypic characterization of human parvovirus B19 in hemophilia patients |
| title_fullStr | Prevalence and genotypic characterization of human parvovirus B19 in hemophilia patients |
| title_full_unstemmed | Prevalence and genotypic characterization of human parvovirus B19 in hemophilia patients |
| title_short | Prevalence and genotypic characterization of human parvovirus B19 in hemophilia patients |
| title_sort | prevalence and genotypic characterization of human parvovirus b19 in hemophilia patients |
| topic | Human parvovirus B19; Hemophilia; Seroprevalence; Blood transfusion; Blood disease; Iran |
| url | https://ijm.tums.ac.ir/index.php/ijm/article/view/3688 |
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