Identification of Apoptotic Oocytes in the Developing Ovary of Embryonic and Post-hatched Chicks in Japanese Quail (Coturnix japonica)
The aim of this study was to examine whether apoptosis of oocytes occurs and their frequency changes during the ovarian development of embryo and post-hatched chicks of Japanese quail. The ovaries of embryos and chicks were processed for paraffin sections and stained by terminal deoxynucleotidyl tr...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Japan Poultry Science Association
2004-01-01
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| Series: | The Journal of Poultry Science |
| Subjects: | |
| Online Access: | https://www.jstage.jst.go.jp/article/jpsa/41/1/41_1_64/_pdf/-char/en |
| _version_ | 1797816973264945152 |
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| author | Yukinori Yoshimura Michiko Nishikori |
| author_facet | Yukinori Yoshimura Michiko Nishikori |
| author_sort | Yukinori Yoshimura |
| collection | DOAJ |
| description | The aim of this study was to examine whether apoptosis of oocytes occurs and their frequency changes during the ovarian development of embryo and post-hatched chicks of Japanese quail. The ovaries of embryos and chicks were processed for paraffin sections and stained by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) method. The TUNEL positive cells were observed in both cortex and medulla of Day 14 embryo and day-old chicks, and there were some TUNEL positive oocyte-like cells in the cortex of day-old chicks. In the 7-day-old chicks many of the oocytes, which grew in size and contained germinal vesicle, were surrounded by follicular wall, and 5.8% oocytes were TUNEL-positive. In 14- to 35-day-old chicks, TUNEL-positive oocytes were negligible and only a few cells in the cortical tissue were stained positive for TUNEL reaction. These results suggest that apoptosis of oocytes occurs at pre- and post-hatching phase, and becomes negligible by Day 14 of post hatching, which probably play a role in eliminating the abnormal oocytes to ensure the quality of oocytes. |
| first_indexed | 2024-03-13T08:45:34Z |
| format | Article |
| id | doaj.art-eb0dcfe827f64fabab188f4bb70a11f8 |
| institution | Directory Open Access Journal |
| issn | 1346-7395 1349-0486 |
| language | English |
| last_indexed | 2024-03-13T08:45:34Z |
| publishDate | 2004-01-01 |
| publisher | Japan Poultry Science Association |
| record_format | Article |
| series | The Journal of Poultry Science |
| spelling | doaj.art-eb0dcfe827f64fabab188f4bb70a11f82023-05-30T06:03:04ZengJapan Poultry Science AssociationThe Journal of Poultry Science1346-73951349-04862004-01-01411646810.2141/jpsa.41.64jpsaIdentification of Apoptotic Oocytes in the Developing Ovary of Embryonic and Post-hatched Chicks in Japanese Quail (Coturnix japonica)Yukinori Yoshimura0Michiko Nishikori1Graduate School of Biosphere Sciences, Hiroshima UniversityGraduate School of Biosphere Sciences, Hiroshima UniversityThe aim of this study was to examine whether apoptosis of oocytes occurs and their frequency changes during the ovarian development of embryo and post-hatched chicks of Japanese quail. The ovaries of embryos and chicks were processed for paraffin sections and stained by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) method. The TUNEL positive cells were observed in both cortex and medulla of Day 14 embryo and day-old chicks, and there were some TUNEL positive oocyte-like cells in the cortex of day-old chicks. In the 7-day-old chicks many of the oocytes, which grew in size and contained germinal vesicle, were surrounded by follicular wall, and 5.8% oocytes were TUNEL-positive. In 14- to 35-day-old chicks, TUNEL-positive oocytes were negligible and only a few cells in the cortical tissue were stained positive for TUNEL reaction. These results suggest that apoptosis of oocytes occurs at pre- and post-hatching phase, and becomes negligible by Day 14 of post hatching, which probably play a role in eliminating the abnormal oocytes to ensure the quality of oocytes.https://www.jstage.jst.go.jp/article/jpsa/41/1/41_1_64/_pdf/-char/enoocytesapoptosisdevelopment of ovarytuneljapanese quail |
| spellingShingle | Yukinori Yoshimura Michiko Nishikori Identification of Apoptotic Oocytes in the Developing Ovary of Embryonic and Post-hatched Chicks in Japanese Quail (Coturnix japonica) The Journal of Poultry Science oocytes apoptosis development of ovary tunel japanese quail |
| title | Identification of Apoptotic Oocytes in the Developing Ovary of Embryonic and Post-hatched Chicks in Japanese Quail (Coturnix japonica) |
| title_full | Identification of Apoptotic Oocytes in the Developing Ovary of Embryonic and Post-hatched Chicks in Japanese Quail (Coturnix japonica) |
| title_fullStr | Identification of Apoptotic Oocytes in the Developing Ovary of Embryonic and Post-hatched Chicks in Japanese Quail (Coturnix japonica) |
| title_full_unstemmed | Identification of Apoptotic Oocytes in the Developing Ovary of Embryonic and Post-hatched Chicks in Japanese Quail (Coturnix japonica) |
| title_short | Identification of Apoptotic Oocytes in the Developing Ovary of Embryonic and Post-hatched Chicks in Japanese Quail (Coturnix japonica) |
| title_sort | identification of apoptotic oocytes in the developing ovary of embryonic and post hatched chicks in japanese quail coturnix japonica |
| topic | oocytes apoptosis development of ovary tunel japanese quail |
| url | https://www.jstage.jst.go.jp/article/jpsa/41/1/41_1_64/_pdf/-char/en |
| work_keys_str_mv | AT yukinoriyoshimura identificationofapoptoticoocytesinthedevelopingovaryofembryonicandposthatchedchicksinjapanesequailcoturnixjaponica AT michikonishikori identificationofapoptoticoocytesinthedevelopingovaryofembryonicandposthatchedchicksinjapanesequailcoturnixjaponica |